LOCUS       sequence-284133-        1595 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1595)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2177
FEATURES             Location/Qualifiers
     source          1..1595
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      8..17
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcggcggc cgccctgagg gaccccgctc agggctatgt gacctttgag
       61 gatgtggctg tctacttctc ccaggaggaa tggagattgc ttgatgacgc tcagaggctc
      121 ctctaccgca atgtgatgct ggagaacttt acacttctgg cctctctggg acttgcatct
      181 tccaagaccc atgaaataac ccagctggag tcatgggagg agcccttcat gcctgcttgg
      241 gaagttgtga cttcagccat accgagaggt tgttggcatg gagccgaggc tgaggaggct
      301 cctgagcaga ttgcttctgt aggactgctc agttcaaaca ttcagcaaca ccagaagcag
      361 cactgtggag agaaaccctt aaaaagacaa gagggcaggg tcccagtttt gaggagttgc
      421 aaagttcacc tatcagagaa gtccttgcaa agcagggagg ttgggaaggc cctcctgatc
      481 agctcaggtg ttctcaagca ccaggtgact cacacaggag agaagtcaca taggagctcc
      541 aaaagtaggg aggcctttca tgctggaaaa aggcattaca aatgcagtga atgtgggaaa
      601 gcctttggtc agaaatattt acttgttcag caccagagac tacatgctgg gaaaaagacg
      661 tatgaatgca gtgaatgtgg gaagttattt agagatatgt ccaacctttt tatacaccaa
      721 atagttcaca ctggagaaag gccttacggg tgtagtaact gtggaaaatc ctttagccgt
      781 aatgctcacc tcattgaaca ccagagagtt cacactggag aaaagccttt tacatgcagt
      841 gaatgtggaa aagctttcag gcataattcc acacttgttc agcatcacaa aatccacact
      901 ggagtaaggc cttatgagtg cagtgaatgt ggaaaattgt ttagtttcaa ctccagcctc
      961 atgaaacatc agagaattca cactggagaa agaccttata agtgcagtga atgtggaaaa
     1021 ttctatagcc acaagtccaa ccttatcaaa cattggcgtg ttcatactgg agaaaggcct
     1081 tacaagtgca gcgactgtgg gaaatttttt acccaatgct caagcctcat gcaacatcaa
     1141 aaagttcaca ctggagaaaa gccttttaag tgcaatgaat gtgggagatt ctttagagag
     1201 aattccaccc tagttagaca tcagagggtt cacactggag caaagcctta tgagtgcagg
     1261 gaatgtggga aattttttag ccaaagctca accctcatgc aacatcgaaa agttcacatt
     1321 ggagaaaagc cttttaagtg caatgaatgt gggagattgt ttagagagaa ttccagcctt
     1381 gttaaacatc agagggttca cactggagca aagccttatg agtgcaggga atgtgggaag
     1441 ttttttcgcc acaactccag tctttttaaa catcgaagga ttcacactgg agaaatgcag
     1501 tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     1561 ggaaaggacg aacgtcgccg gctgtgctta tccct
//
