LOCUS       sequence-284136-        1418 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1418)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2205
FEATURES             Location/Qualifiers
     source          1..1418
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggagtctcc aagaactgct aaaggaggaa gggatatcaa gatgagtggg
       61 gatgtagccg attccacgga tgctcgcagc actctcagcc aggtggagcc aggaaatgat
      121 cgaaatggcc tagatttcaa caggcagatt aaaaccgaag atctcagtga ctccctgcag
      181 cagaccctct cccatcggcc atgccacctg agtcaaggac ctgccatgat gtccggaaac
      241 caaatgtctg ggctaaatgc cagcccatgt caggacatgg cttccctcca tccgctccag
      301 cagcttgtgc tggttcccgg ccacttacag tctgtatccc agttcctgct atctcagacc
      361 cagcctgggc agcaaggtct gcagccaaat ctcctcccct ttccacagca acaaagcggt
      421 ctcctcctcc cacagactgg gccgggactg gcatcccagg catttgggca ccctgggctg
      481 ccaggatcct ctttagaacc ccacctggaa gcatcccagc atctcccagt gcccaagcat
      541 ctacccagct ctggaggggc cgatgagccc agtgacctcg aggagctgga gaagtttgcc
      601 aagaccttca agcagaggcg cattaagctg ggcttcacac agggagatgt ggggctggcg
      661 atgggaaagc tgtatggcaa cgacttcagc cagaccacca tctcacgatt tgaggccctc
      721 aacctgagct tcaagaacat gtgcaagctc aagcccctgc tggagaagtg gctgaatgat
      781 gcagagtcct ctccgtcaga cccctcagtg agcacgccca gctcctaccc cagcctcagt
      841 gaagtatttg gtaggaagag aaagaaacgg accagcatcg agaccaacat ccgcctgact
      901 ctggagaaga ggtttcaaga taacccaaaa cccagctcgg aggagatctc catgattgca
      961 gagcagttgt ccatggagaa ggaggtggtg agggtctggt tctgcaaccg acgccaaaag
     1021 gagaagcgaa tcaactgccc tgtggccaca cccatcaaac cacctgtcta caactcccgg
     1081 ctggtatctc cctcagggtc tctgggcccc ctctctgtcc ctcctgtcca cagtaccatg
     1141 cctggaacag taacgtcatc ctgttcccct gggaacaaca gcaggccttc atctcctggc
     1201 tcaggactcc acgccagcag ccccactgca tctcaaaata actccaaagc agcagtgaac
     1261 tccgcctcca gttttaactc ttcaggatct tggtaccgat ggaatcattc cacctacctc
     1321 cactagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
     1381 tgtggaaagg acgagggccg caagagttat cggacaca
//
