LOCUS       sequence-284142-        1613 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1613)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2229
FEATURES             Location/Qualifiers
     source          1..1613
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggagcgtt gacatttaga gatgtggcca tagaattctc tctggaggag
       61 tggcaatgtc tggacaccga acaacagaat ttatatagaa atgtaatgtt agataactac
      121 agaaacctgg tcttcctggg tattgctgtc tcaaagccag acctgatcac ctgtctggag
      181 caagaaaaag agccttggaa tttgaagaca catgatatgg tagccaaacc cccagttata
      241 tgttctcata ttgcccaaga cctttggcca gagcaaggca taaaagatta tttccaagaa
      301 gtcatactga gacaatataa aaaatgtaga catgagaatt tactgttaag aaaaggctgt
      361 aaaaatgtgg atgagtttaa gatgcacaaa aaaggttata atagacataa ccagtgtttg
      421 acaacttccc atagcaaaat atttcagtgt gacaaatatg tgaaagtctt tcataaattt
      481 tcaaattcaa acagacataa gataagacat acttcgaaga aacctttcaa atgtaaagaa
      541 tgtggaaaat tattttgcat tctttcacac ttagctcaac ataaaaaaat tcatactgga
      601 gagaaatcct acaaatgtga agaatatggc aaagccttta atgagtcctc aaactgtact
      661 acacataaaa gaattactga gaaaaaacct tacaaatgta aagaatgtgg caaagccttt
      721 aactggtttt cacattttac tacacataag agaattcata ctggagaaaa accctaccaa
      781 tgtgagaaat gtggcaaatt ttttaaccaa tccacaaacc ttactacaca taaaagaatt
      841 catactggag agaaacccta taaatgtgaa gaatgtggca aagcctttaa ccagtcctca
      901 aaccttactg aacataagaa aattcatact aaagagcaac catacaaatg cgaaaaatgt
      961 ggcaaagctt ttaagtggtc ctcaaccctt acaaaacata aaagaattca taatggagaa
     1021 aaaccctaca aatgtgaaga atgtggcaaa gcttttaacc gatcctcaac ccttaataga
     1081 cataagataa ctcatactgg agggaaaccc tacaaatata aagaatgtgg taaagctttt
     1141 aaccaatcct caactcttac tatacataag ataattcata ctgtagagaa attttacaaa
     1201 tgtgaagaat gtggcaaagc ctttagccgt atctcacacc ttactacaca taagagaatt
     1261 catactggag agaaacccta caaatgtgaa gaatgtggca gagctttcaa ccagtcctca
     1321 acccttacta cacataaaag aattcatact ggagagaaac cctatgaatg tgaagaatgt
     1381 ggcaaagctt ttaaccggtc ctcaaccctc actacacata agataattca ttctggggaa
     1441 aaaatctaca aatgtaaaga atgtggtaaa gcctttaggc ggttctcaca ccttactagg
     1501 cataagacaa ttcatacata gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
     1561 tggctttata tatcttgtgg aaaggacgag gccgaggcgc agtgcagcct ctc
//
