LOCUS       sequence-284153-       14053 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14053)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 14053)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2324
FEATURES             Location/Qualifiers
     source          1..14053
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(5688..5702)
                     /codon_start=1
                     /product="tag for protein cleavage using Ni2+ ion (Dang et 
                     al., 2019)"
                     /label=SNAC tag
                     /translation="GSHHW"
     misc_feature    9910..10498
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9963..9983)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10381..10392)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10570..10803
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10881..11002
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10918..10937)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10972..10991
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      11042..11177
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(11197..11216)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11198..11216)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11226..11243)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11226..11242)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11235..11257)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      11384..11839
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11471..11490)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11681..11702
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        11865..11969
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11970..12038,12039..12830)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12188..12207)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      13001..13589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13490..13509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13743..13760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    13877..13898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(13913..13918,13919..13936,13937..13943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13951..13967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13956..13978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13975..13991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13975..13991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     14010..14030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        14012..14030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggga cccggctgcg atttgctgct
     4141 gcggacagca gctaccatca ctgctgccgc catcatgtca gacacggaca gcgacgaaga
     4201 ttccgctgga ggcggcccat tttctttagc gggtttcctt ttcggcaaca tcaatggagc
     4261 cgggcagctg gagggggaaa gcgtcttgga tgatgaatgt aagaagcact tggcaggctt
     4321 gggggctttg gggctgggca gcctgatcac tgaactcacg gcaaatgaag aattgaccgg
     4381 gactgacggt gccttggtaa atgatgaagg gtgggttagg agtacagaag atgctgtgga
     4441 ctattcagac atcaatgagg tggcagaaga tgaaagccga agataccagc agacgatggg
     4501 gagcttgcag cccctttgcc actcagatta tgatgaagat gactatgatg ctgattgtga
     4561 agacattgat tgcaagttga tgcctcctcc acctccaccc ccgggaccaa tgaagaagga
     4621 taaggaccag gattctatta ctggtgtgtc tgaaaatgga gaaggcatca tcttgccctc
     4681 catcattgcc ccttcctctt tggcctcaga gaaagtggac ttcagtagtt cctctgactc
     4741 agaatctgag atgggacctc aggaagcaac acaggcagaa tctgaagatg gaaagctgac
     4801 ccttccattg gctgggatta tgcagcatga tgccaccaag ctgttgccaa gtgtcacaga
     4861 actttttcca gaatttcgac ctggaaaggt gttacgtttt ctacgtcttt ttggaccagg
     4921 gaagaatgtc ccatctgttt ggcggagtgc tcggagaaag aggaagaaga agcaccgtga
     4981 gctgatacag gaagagcaga tccaggaggt ggagtgctca gtagaatcag aagtcagcca
     5041 gaagtctttg tggaactacg actacgctcc accaccacct ccagagcagt gtctctctga
     5101 tgatgaaatc acgatgatgg ctcctgtgga gtccaaattt tcccaatcaa ctggagatat
     5161 agataaagtg acagatacca aaccaagagt ggctgagtgg cgttatgggc ctgcccgact
     5221 gtggtatgat atgctgggtg tccctgaaga tggcagtggg tttgactatg gcttcaaact
     5281 gagaaagaca gaacatgaac ctgtgataaa atctagaatg atagaggaat ttaggaaact
     5341 tgaggaaaac aatggcactg atcttctggc tgatgaaaac ttcctgatgg tgacacagct
     5401 gcattgggag gatgatatca tctgggatgg ggaggatgtc aaacacaaag ggacaaaacc
     5461 tcagcgtgca agcctggcag gctggcttcc ttctagcatg actaggaatg cgatggctta
     5521 caatgttcag caaggttttg cagccactct tgatgatgac aaaccttggt actccatttt
     5581 tcccattgac aatgaggatc tggtatatgg acgctgggag gacaatatca tttgggatgc
     5641 tcaggccatg ccccggctgt tggaacctcc tgttttgaca cttgatccca atgatgagaa
     5701 cctcattttg gaaattcctg atgagaagga agaggccacc tctaactccc cctccaagga
     5761 gagtaagaag gaatcatctc tgaagaagag tcgaattctc ttagggaaaa caggagtcat
     5821 caaggaggaa ccacagcaga acatgtctca gccagaagtg aaagatccat ggaatctctc
     5881 caatgatgag tattattatc ccaagcaaca gggtcttcga ggcacctttg gagggaatat
     5941 tatccagcat tcaattcctg ctgtggaatt acggcagccc ttctttccca cccacatggg
     6001 gcccatcaaa ctccggcagt tccatcgccc acctctgaaa aagtactcat ttggtgcact
     6061 ttctcagcca ggtccccact cagtccaacc tttgctaaag cacatcaaaa aaaaggccaa
     6121 gatgagagaa caagagaggc aagcttcagg tggtggagag atgtttttta tgcgcacacc
     6181 tcaggacctc acaggcaaag atggtgatct tattcttgca gaatatagtg aggaaaatgg
     6241 acccttaatg atgcaggttg gcatggcaac caagataaag aactattata aacggaaacc
     6301 tggaaaagat cctggagcac cagattgtaa atatggggaa actgtttact gccatacatc
     6361 tcctttcctg ggttctctcc atcctggcca attgctgcaa gcatttgaga acaacctttt
     6421 tcgtgctcca atttatcttc ataagatgcc agaaactgat ttcttgatca ttcggacaag
     6481 acagggttac tatattcggg aattagtgga tatttttgtg gttggccagc agtgtccctt
     6541 gtttgaagtt cctgggccta actccaaaag ggccaatacg catattcgag actttctaca
     6601 ggtttttatt taccgccttt tctggaaaag taaagatcgg ccacggagga tacgaatgga
     6661 agatataaaa aaagcctttc cttcccattc agaaagcagc atccggaaga ggctaaagct
     6721 ctgcgctgac ttcaaacgca cagggatgga ctcaaactgg tgggtgctta agtctgattt
     6781 tcgtttacca acggaagaag agatcagagc tatggtgtca ccagagcagt gctgtgctta
     6841 ttatagcatg atagctgcag agcaacgact gaaggatgct ggctatggtg agaaatcctt
     6901 ttttgctcca gaagaagaaa atgaggaaga tttccagatg aagattgatg atgaagttcg
     6961 cactgcccct tggaacacca caagggcctt cattgctgcc atgaagggca agtgtctgct
     7021 agaggtgact ggggtggcag atcccacggg gtgtggtgaa ggattctcct atgtgaagat
     7081 tccaaacaaa ccaacacagc agaaggatga taaagaaccg cagccagtga agaagacagt
     7141 gacaggaaca gatgcagacc ttcgtcgcct ttccctgaaa aatgccaagc aacttctacg
     7201 taaatttggt gtgcctgagg aagagattaa aaagttgtcc cgctgggaag tgattgatgt
     7261 ggtgcgcaca atgtcaacag aacaggctcg ttctggagag gggcccatga gtaaatttgc
     7321 ccgtggatca aggttttctg tggctgagca tcaagagcgt tacaaagagg aatgtcagcg
     7381 catctttgac ctacagaaca aggttctgtc atcaactgaa gtcttatcaa ctgacacaga
     7441 cagcagctca gctgaagata gtgactttga agaaatggga aagaacattg agaacatgtt
     7501 gcagaacaag aaaaccagct ctcagctttc acgtgaacgg gaggaacagg agcggaagga
     7561 actacagcga atgctactgg cagcaggctc agcagcatcc ggaaacaatc acagagatga
     7621 tgacacagct tccgtgacta gccttaactc ttctgccact ggacgctgtc tcaagattta
     7681 tcgcacgttt cgagatgaag aggggaaaga gtatgttcgc tgtgagacag tccgaaaacc
     7741 agctgtcatt gatgcctatg tgcgcatacg gactacaaaa gatgaggaat tcattcgaaa
     7801 atttgccctt tttgatgaac aacatcggga agagatgcga aaagaacggc ggaggattca
     7861 agagcaactg aggcggctta agaggaacca ggaaaaggag aagcttaagg gtcctcctga
     7921 gaagaagccc aagaaaatga aggagcgtcc tgacctaaaa ctgaaatgtg gggcatgtgg
     7981 tgccattgga cacatgagga ctaacaaatt ctgccccctc tattatcaaa caaatgcgcc
     8041 accttccaac cctgttgcca tgacagaaga acaggaggag gagttggaaa agacagtcat
     8101 tcataatgat aatgaagaac ttatcaaggt tgaagggacc aaaattgtct tggggaaaca
     8161 gctaattgag agtgcggatg aggttcgcag aaaatctctg gttctcaagt ttcctaaaca
     8221 gcagcttcct ccaaagaaga aacggcgagt tggaaccact gttcactgtg actatttgaa
     8281 tagacctcat aagtccatcc accggcgccg cacagaccct atggtgacgc tgtcgtccat
     8341 cttggagtct atcatcaatg acatgagaga tcttccaaat acataccctt tccacactcc
     8401 agtcaatgca aaggttgtaa aggactacta caaaatcatc actcggccaa tggacctaca
     8461 aacactccgc gaaaacgtgc gtaaacgcct ctacccatct cgggaagagt tcagagagca
     8521 tctggagcta attgtgaaaa atagtgcaac ctacaatggg ccaaaacact cattgactca
     8581 gatctctcaa tccatgctgg atctctgtga tgaaaaactc aaagagaaag aagacaaatt
     8641 agctcgctta gagaaagcta tcaacccctt gctggatgat gatgaccaag tggcgttttc
     8701 tttcattctg gacaacattg tcacccagaa aatgatggca gttccagatt cttggccatt
     8761 tcatcaccca gttaataaga aatttgttcc agattattac aaagtgattg tcaatccaat
     8821 ggatttagag accatacgta agaacatctc caagcacaag tatcagagtc gggagagctt
     8881 tctggatgat gtaaacctta ttctggccaa cagtgttaag tataatggac ctgagagtca
     8941 gtatactaag actgcccagg agattgtgaa cgtctgttac cagacattga ctgagtatga
     9001 tgaacatttg actcaacttg agaaggatat ttgtactgct aaagaagcag ctttggagga
     9061 agcagaatta gaaagcctgg acccaatgac cccagggccc tacacgcctc aggctaagcc
     9121 tcctgatttg tatgatacca acacatccct cagtatgtct cgagatgcct ctgtatttca
     9181 agatgagagc aatatgtctg tcttggatat tcccagtgcc actccagaaa agcaggtaac
     9241 acaggaaggt gaagatggag atggtgatct tgcagatgaa gaggaaggaa ctgtacaaca
     9301 gcctcaagcc agtgtcctgt atgaggattt gcttatgtct gaaggagaag atgatgagga
     9361 agatgctggg agtgatgaag aaggagacaa tcctttctct gctatccagc tgagtgaaag
     9421 tggaagtgac tctgatgtgg gatctggtgg aataagaccc aaacaacccc gcatgcttca
     9481 ggagaacaca aggatggaca tggaaaatga agaaagcatg atgtcctatg agggagacgg
     9541 tggggaggct tcccatggtt tggaggatag caacatcagt tatgggagct atgaggagcc
     9601 tgatcccaag tcgaacaccc aagacacaag cttcagcagc atcggtgggt atgaggtatc
     9661 agaggaggaa gaagatgagg aggaggaaga gcagcgctct gggccgagcg tactaagcca
     9721 ggtccacctg tcagaggacg aggaggacag tgaggatttc cactccattg ctggggacag
     9781 tgacttggac tctgatgaat agtaatgaac tagtccgtaa cttgaaagta tttcgatttc
     9841 ttggctttat atatcttgtg gaaaggacga aacttattta tactagcaga ggtcacgcgt
     9901 taagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     9961 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
    10021 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
    10081 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
    10141 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
    10201 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
    10261 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
    10321 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
    10381 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
    10441 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcgt
    10501 cgactttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
    10561 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta
    10621 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
    10681 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
    10741 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
    10801 gcagtacgta tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
    10861 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
    10921 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
    10981 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc
    11041 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
    11101 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
    11161 aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg
    11221 cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
    11281 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
    11341 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc
    11401 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
    11461 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
    11521 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
    11581 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
    11641 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
    11701 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
    11761 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
    11821 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
    11881 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
    11941 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
    12001 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
    12061 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
    12121 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
    12181 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
    12241 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
    12301 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
    12361 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
    12421 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
    12481 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
    12541 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
    12601 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
    12661 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
    12721 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
    12781 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
    12841 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
    12901 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
    12961 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
    13021 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
    13081 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
    13141 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
    13201 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
    13261 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
    13321 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
    13381 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
    13441 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
    13501 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
    13561 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
    13621 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
    13681 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
    13741 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
    13801 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
    13861 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
    13921 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
    13981 acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc
    14041 tggagctgca agc
//
