LOCUS       sequence-284157-        5783 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5783)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 5783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2325
FEATURES             Location/Qualifiers
     source          1..5783
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(1583..1597)
                     /codon_start=1
                     /product="tag for protein cleavage using Ni2+ ion (Dang et 
                     al., 2019)"
                     /label=SNAC tag
                     /translation="GSHHW"
ORIGIN
        1 accgccacca tgggacccgg ctgcgatttg ctgctgcgga cagcagctac catcactgct
       61 gccgccatca tgtcagacac ggacagcgac gaagattccg ctggaggcgg cccattttct
      121 ttagcgggtt tccttttcgg caacatcaat ggagccgggc agctggaggg ggaaagcgtc
      181 ttggatgatg aatgtaagaa gcacttggca ggcttggggg ctttggggct gggcagcctg
      241 atcactgaac tcacggcaaa tgaagaattg accgggactg acggtgcctt ggtaaatgat
      301 gaagggtggg ttaggagtac agaagatgct gtggactatt cagacatcaa tgaggtggca
      361 gaagatgaaa gccgaagata ccagcagacg atggggagct tgcagcccct ttgccactca
      421 gattatgatg aagatgacta tgatgctgat tgtgaagaca ttgattgcaa gttgatgcct
      481 cctccacctc cacccccggg accaatgaag aaggataagg accaggattc tattactggt
      541 gtgtctgaaa atggagaagg catcatcttg ccctccatca ttgccccttc ctctttggcc
      601 tcagagaaag tggacttcag tagttcctct gactcagaat ctgagatggg acctcaggaa
      661 gcaacacagg cagaatctga agatggaaag ctgacccttc cattggctgg gattatgcag
      721 catgatgcca ccaagctgtt gccaagtgtc acagaacttt ttccagaatt tcgacctgga
      781 aaggtgttac gttttctacg tctttttgga ccagggaaga atgtcccatc tgtttggcgg
      841 agtgctcgga gaaagaggaa gaagaagcac cgtgagctga tacaggaaga gcagatccag
      901 gaggtggagt gctcagtaga atcagaagtc agccagaagt ctttgtggaa ctacgactac
      961 gctccaccac cacctccaga gcagtgtctc tctgatgatg aaatcacgat gatggctcct
     1021 gtggagtcca aattttccca atcaactgga gatatagata aagtgacaga taccaaacca
     1081 agagtggctg agtggcgtta tgggcctgcc cgactgtggt atgatatgct gggtgtccct
     1141 gaagatggca gtgggtttga ctatggcttc aaactgagaa agacagaaca tgaacctgtg
     1201 ataaaatcta gaatgataga ggaatttagg aaacttgagg aaaacaatgg cactgatctt
     1261 ctggctgatg aaaacttcct gatggtgaca cagctgcatt gggaggatga tatcatctgg
     1321 gatggggagg atgtcaaaca caaagggaca aaacctcagc gtgcaagcct ggcaggctgg
     1381 cttccttcta gcatgactag gaatgcgatg gcttacaatg ttcagcaagg ttttgcagcc
     1441 actcttgatg atgacaaacc ttggtactcc atttttccca ttgacaatga ggatctggta
     1501 tatggacgct gggaggacaa tatcatttgg gatgctcagg ccatgccccg gctgttggaa
     1561 cctcctgttt tgacacttga tcccaatgat gagaacctca ttttggaaat tcctgatgag
     1621 aaggaagagg ccacctctaa ctccccctcc aaggagagta agaaggaatc atctctgaag
     1681 aagagtcgaa ttctcttagg gaaaacagga gtcatcaagg aggaaccaca gcagaacatg
     1741 tctcagccag aagtgaaaga tccatggaat ctctccaatg atgagtatta ttatcccaag
     1801 caacagggtc ttcgaggcac ctttggaggg aatattatcc agcattcaat tcctgctgtg
     1861 gaattacggc agcccttctt tcccacccac atggggccca tcaaactccg gcagttccat
     1921 cgcccacctc tgaaaaagta ctcatttggt gcactttctc agccaggtcc ccactcagtc
     1981 caacctttgc taaagcacat caaaaaaaag gccaagatga gagaacaaga gaggcaagct
     2041 tcaggtggtg gagagatgtt ttttatgcgc acacctcagg acctcacagg caaagatggt
     2101 gatcttattc ttgcagaata tagtgaggaa aatggaccct taatgatgca ggttggcatg
     2161 gcaaccaaga taaagaacta ttataaacgg aaacctggaa aagatcctgg agcaccagat
     2221 tgtaaatatg gggaaactgt ttactgccat acatctcctt tcctgggttc tctccatcct
     2281 ggccaattgc tgcaagcatt tgagaacaac ctttttcgtg ctccaattta tcttcataag
     2341 atgccagaaa ctgatttctt gatcattcgg acaagacagg gttactatat tcgggaatta
     2401 gtggatattt ttgtggttgg ccagcagtgt cccttgtttg aagttcctgg gcctaactcc
     2461 aaaagggcca atacgcatat tcgagacttt ctacaggttt ttatttaccg ccttttctgg
     2521 aaaagtaaag atcggccacg gaggatacga atggaagata taaaaaaagc ctttccttcc
     2581 cattcagaaa gcagcatccg gaagaggcta aagctctgcg ctgacttcaa acgcacaggg
     2641 atggactcaa actggtgggt gcttaagtct gattttcgtt taccaacgga agaagagatc
     2701 agagctatgg tgtcaccaga gcagtgctgt gcttattata gcatgatagc tgcagagcaa
     2761 cgactgaagg atgctggcta tggtgagaaa tccttttttg ctccagaaga agaaaatgag
     2821 gaagatttcc agatgaagat tgatgatgaa gttcgcactg ccccttggaa caccacaagg
     2881 gccttcattg ctgccatgaa gggcaagtgt ctgctagagg tgactggggt ggcagatccc
     2941 acggggtgtg gtgaaggatt ctcctatgtg aagattccaa acaaaccaac acagcagaag
     3001 gatgataaag aaccgcagcc agtgaagaag acagtgacag gaacagatgc agaccttcgt
     3061 cgcctttccc tgaaaaatgc caagcaactt ctacgtaaat ttggtgtgcc tgaggaagag
     3121 attaaaaagt tgtcccgctg ggaagtgatt gatgtggtgc gcacaatgtc aacagaacag
     3181 gctcgttctg gagaggggcc catgagtaaa tttgcccgtg gatcaaggtt ttctgtggct
     3241 gagcatcaag agcgttacaa agaggaatgt cagcgcatct ttgacctaca gaacaaggtt
     3301 ctgtcatcaa ctgaagtctt atcaactgac acagacagca gctcagctga agatagtgac
     3361 tttgaagaaa tgggaaagaa cattgagaac atgttgcaga acaagaaaac cagctctcag
     3421 ctttcacgtg aacgggagga acaggagcgg aaggaactac agcgaatgct actggcagca
     3481 ggctcagcag catccggaaa caatcacaga gatgatgaca cagcttccgt gactagcctt
     3541 aactcttctg ccactggacg ctgtctcaag atttatcgca cgtttcgaga tgaagagggg
     3601 aaagagtatg ttcgctgtga gacagtccga aaaccagctg tcattgatgc ctatgtgcgc
     3661 atacggacta caaaagatga ggaattcatt cgaaaatttg ccctttttga tgaacaacat
     3721 cgggaagaga tgcgaaaaga acggcggagg attcaagagc aactgaggcg gcttaagagg
     3781 aaccaggaaa aggagaagct taagggtcct cctgagaaga agcccaagaa aatgaaggag
     3841 cgtcctgacc taaaactgaa atgtggggca tgtggtgcca ttggacacat gaggactaac
     3901 aaattctgcc ccctctatta tcaaacaaat gcgccacctt ccaaccctgt tgccatgaca
     3961 gaagaacagg aggaggagtt ggaaaagaca gtcattcata atgataatga agaacttatc
     4021 aaggttgaag ggaccaaaat tgtcttgggg aaacagctaa ttgagagtgc ggatgaggtt
     4081 cgcagaaaat ctctggttct caagtttcct aaacagcagc ttcctccaaa gaagaaacgg
     4141 cgagttggaa ccactgttca ctgtgactat ttgaatagac ctcataagtc catccaccgg
     4201 cgccgcacag accctatggt gacgctgtcg tccatcttgg agtctatcat caatgacatg
     4261 agagatcttc caaatacata ccctttccac actccagtca atgcaaaggt tgtaaaggac
     4321 tactacaaaa tcatcactcg gccaatggac ctacaaacac tccgcgaaaa cgtgcgtaaa
     4381 cgcctctacc catctcggga agagttcaga gagcatctgg agctaattgt gaaaaatagt
     4441 gcaacctaca atgggccaaa acactcattg actcagatct ctcaatccat gctggatctc
     4501 tgtgatgaaa aactcaaaga gaaagaagac aaattagctc gcttagagaa agctatcaac
     4561 cccttgctgg atgatgatga ccaagtggcg ttttctttca ttctggacaa cattgtcacc
     4621 cagaaaatga tggcagttcc agattcttgg ccatttcatc acccagttaa taagaaattt
     4681 gttccagatt attacaaagt gattgtcaat ccaatggatt tagagaccat acgtaagaac
     4741 atctccaagc acaagtatca gagtcgggag agctttctgg atgatgtaaa ccttattctg
     4801 gccaacagtg ttaagtataa tggacctgag agtcagtata ctaagactgc ccaggagatt
     4861 gtgaacgtct gttaccagac attgactgag tatgatgaac atttgactca acttgagaag
     4921 gatatttgta ctgctaaaga agcagctttg gaggaagcag aattagaaag cctggaccca
     4981 atgaccccag ggccctacac gcctcagcct cctgatttgt atgataccaa cacatccctc
     5041 agtatgtctc gagatgcctc tgtatttcaa gatgagagca atatgtctgt cttggatatt
     5101 cccagtgcca ctccagaaaa gcaggtaaca caggaaggtg aagatggaga tggtgatctt
     5161 gcagatgaag aggaaggaac tgtacaacag cctcaagcca gtgtcctgta tgaggatttg
     5221 cttatgtctg aaggagaaga tgatgaggaa gatgctggga gtgatgaaga aggagacaat
     5281 cctttctctg ctatccagct gagtgaaagt ggaagtgact ctgatgtggg atctggtgga
     5341 ataagaccca aacaaccccg catgcttcag gagaacacaa ggatggacat ggaaaatgaa
     5401 gaaagcatga tgtcctatga gggagacggt ggggaggctt cccatggttt ggaggatagc
     5461 aacatcagtt atgggagcta tgaggagcct gatcccaagt cgaacaccca agacacaagc
     5521 ttcagcagca tcggtgggta tgaggtatca gaggaggaag aagatgagga ggaggaagag
     5581 cagcgctctg ggccgagcgt actaagccag gtccacctgt cagaggacga ggaggacagt
     5641 gaggatttcc actccattgc tggggacagt gacttggact ctgatgaata gtaatgaact
     5701 agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgac
     5761 gtcgaaaccg ttcaacggaa gga
//
