LOCUS       sequence-284163-        2030 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2336
FEATURES             Location/Qualifiers
     source          1..2030
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggactcagt ctcctttgag gatgtggccg tgaacttcac cctggaggag
       61 tgggctttgc tggattcttc acagaaaaag ctctatgaag atgtgatgca ggagaccttc
      121 aaaaacctgg tttgtctagg aaaaaagtgg gaagaccagg acattgaaga tgaccacaga
      181 aaccagggga aaaatcgaag atgtcatatg gttgagagac tctgtgaaag tagaagaggt
      241 agcaaatgtg gagaaaccac tagccagatg ccaaatgtta atatcaacaa ggaaactttt
      301 actggagcaa aaccacatga atgcagcttt tgtggaagag acttcattca tcattcgtcc
      361 cttaataggc acatgagatc tcacactgga cagaaaccaa atgagtatca ggaatatgaa
      421 aagcaaccat gtaaatgtaa agcagttggg aaaaccttca gttatcacca ctgctttcgc
      481 aaacatgaaa gaactcacac tggagtgaag ccctatgaat gtaaacagtg tgggaaagcc
      541 tttatatatt accagccatt tcaaagacat gaaaggactc atgctggaca gaaaccctat
      601 gaatgtaagc aatgtggaaa aacctttata tattaccagt cttttcaaaa acatgctcat
      661 actggaaaga aaccctatga atgtaaacag tgtgggaaag cctttatatg ttaccaatct
      721 tttcaaagac acaaaaggac tcacactgga gagaaaccct atgaatgtaa gcaatgtggt
      781 aaggctttca gttgtcccac atactttcga actcatgaaa gaactcacac tggagaaaaa
      841 ccctacaaat gtaaagaatg tggtaaagcc ttcagttttc tcagttcttt tcgaaggcat
      901 aaaaggactc atagtggaga gaaaccctat gaatgtaaag aatgtggaaa agccttcttt
      961 tattctgcaa gctttcgagc acatgtaata atacacactg gggctcgacc ttataaatgt
     1021 aaagaatgtg ggaaagcctt caactcttct aattcctgtc gagtgcatga aagaactcat
     1081 attggagaaa aaccatatga atgtaaacga tgtggcaaat cattcagttg gtccatttct
     1141 cttcgattgc atgaaagaac tcatactgga gagaaacctt atgagtgtaa acagtgtcat
     1201 aaaaccttca gtttttcaag ttcccttcga gaacacgaaa caactcacac tggagagaaa
     1261 ccctatgaat gtaaacaatg tggtaaaacc ttcagttttt caagttccct tcaaagacat
     1321 gaaaggactc acaatgcaga gaaaccctat gaatgtaaac agtgtgggaa agccttcagg
     1381 tgttcaagtt attttcgaat tcatgaaagg tcacacactg gagagaaacc ctatgaatgt
     1441 aaacagtgtg gaaaagtttt cattcgttcc agttcctttc gactgcatga aagaacacac
     1501 actggagaga aaccctatga atgtaaacta tgcggtaaaa ccttcagttt ttcaagttcc
     1561 cttcgagaac atgaaaaaat tcacactgga aataagcctt ttgagtgtaa gcaatgtggt
     1621 aaggccttcc ttcgttccag tcaaattcga ttgcatgaaa ggactcacac tggagagaaa
     1681 ccgtatcaat gtaaacaatg tggaaaagcc ttcatttctt ccagtaaatt tcgaatgcat
     1741 gagagaactc acacgggaga gaaaccctat cgatgtaaac aatgtgggaa agccttcaga
     1801 ttttcaagtt ctgttcgaat tcatgaaagg tctcacactg gagagaaacc ttatgaatgc
     1861 aaacaatgtg gaaaagcctt catttcttcc agtcactttc gactgcatga aaggactcat
     1921 atgggagaga aagtctagta atgaactagt ccgtaacttg aaagtatttc gatttcttgg
     1981 ctttatatat cttgtggaaa ggacgaggtt actagcggac cggtaaatat
//
