LOCUS       sequence-284184-        7622 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7622)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 7622)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2388
FEATURES             Location/Qualifiers
     source          1..7622
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(6938..6952)
                     /codon_start=1
                     /product="tag for protein cleavage using Ni2+ ion (Dang et 
                     al., 2019)"
                     /label=SNAC tag
                     /translation="GSHHW"
ORIGIN
        1 accgccacca tggaggtgct tcagtgtgat ggctgtgatt tccgagcccc gtcttatgaa
       61 gatctcaagg cacacattca ggatgtccac acggcatttc tgcagccaac tgatgttgct
      121 gaggacaatg tgaatgagct acgatgtggg tccgtgaatg ccagtaatca gacagaggtg
      181 gagttttctt ctataaagga tgaatttgcc attgcagaag atttatcagg tcaaaatgca
      241 acttcattgg ggaccggagg ttactatggc cacagtccag gatattatgg tcagcatatt
      301 gccgctaatc ccaaaccaac aaacaagttt tttcaatgca agttctgtgt acgctacttc
      361 aggtcaaaaa acctcctcat agaacacact agaaaggtcc atggagctca agctgaaggg
      421 agttcatcag gaccccctgt cccgggatcc ttaaattata atatcatgat gcacgaggga
      481 tttggaaagg tcttctcttg ccagttttgc acatacaagt caccaagaag ggcaagaata
      541 attaagcatc agaagatgta tcacaaaaac aatttgaagg agaccactgc tcccccacct
      601 gctcctgctc caatgccaga ccctgtggtt ccgcccgtat cactgcagga cccctgcaag
      661 gaactgccag cagaggttgt ggagcgcagc atcttagagt ctatggtcaa gcctttgacc
      721 aaatctcgag gcaacttttg ttgtgagtgg tgcagctacc agaccccccg ccgagaacgc
      781 tggtgtgacc acatgatgaa gaaacaccgc agtatggtca agatcctttc cagtctcaga
      841 cagcaacaag aaggaactaa tctacctgat gtgccgaaca agagtgcccc cagccccact
      901 tccaactcca cctatctgac catgaatgct gcaagccggg agatacccaa tactaccgtc
      961 tccaacttca ggggctccat gggcaactcc atcatgagac ccaattcttc agcttccaag
     1021 ttttcgccca tgtcttaccc tcagatgaag ccgaagtcac ctcacaattc tggtctagtt
     1081 aacttgacag agagatcccg ttatggaatg actgacatga ccaattcttc tgctgacctg
     1141 gaaactaaca gcatgctaaa tgactctagt tctgatgaag agttaaatga aatagacagt
     1201 gagaatggtt taagtgctat ggatcaccag acatcaggcc tgtctgcaga gcagctgatg
     1261 ggctcagatg gcaacaaatt attggagacc aaggggattc catttagaag attcatgaat
     1321 aggttccagt gccccttttg tcctttcctc accatgcatc gacgtagcat ctctcgtcac
     1381 atagaaaaca tccacttatc tggaaagaca gctgtctaca aatgtgacga atgtccgttt
     1441 acttgcaaga gctcgttgaa acttggggct cacaaacagt gtcacacggg tacaacgtca
     1501 gattgggatg ctgtgaattc ccagagtgaa agcatttctt cctcactgaa tgaaggtgtg
     1561 gtgtcttatg agagctcaag catcaatggt agaaagtcag gagtcatgtt ggatcccttg
     1621 cagcagcaac agccaccgca gccaccacca ccgccgccgc caccaccacc atcacagcca
     1681 cagccactgc agcagccaca gccaccacag ctgcagccac cacatcaggt gccaccccag
     1741 ccacaaacac agccaccacc aacgcagcag ccacagccac ccacacaagc cgcacctctg
     1801 cacccataca aatgcaccat gtgtaattac tccaccacaa ctctgaaagg gctaagagtc
     1861 catcagcagc ataaacattc attctgtgac aacttgccaa aattcgaggg gcagccctca
     1921 agcctaccat tggaaaatga gacagacagc cacccctctt ccagcaacac tgtgaagaaa
     1981 agtcagacct caattcttgg gttgtcctcc aagaacaatt ttgtagctaa agcctctagg
     2041 aagctcgcca atgactttcc tctagatttg tcacccgtga agaagagaac caggattgac
     2101 gagatagcaa gcaaccttca gagcaaaatt aaccaaacca aacagcagga agatgcagtg
     2161 atcaatgttg aggatgatga agaggaagag gaagacaacg aagtcgagat agaggttgag
     2221 ttggacaggg aggaagaacc gacagaaccc atcatagagg ttcccacttc cttttctgcc
     2281 caacagatat gggtaagaga taccagtgag ccccagaaag agcccaactt cagaaacatc
     2341 acccacgatt acaatgccac caatggggct gagattgagc tcaccctttc tgaagatgaa
     2401 gaggattatt atggctcctc aacaaacttg aaagatcacc aagtttccaa tactgctctg
     2461 ctgaataccc aaactcccat ctatgggact gagcacaata gtgaaaacac agactttggt
     2521 gactctggaa ggctttacta ttgtaaacac tgtgacttta acaacaaatc tgcccggagt
     2581 gttagcaccc actaccaacg aatgcaccca tacattaaat tcagctttag gtacatcttg
     2641 gaccccaatg atcacagtgc agtgtacagg tgcctggaat gctacatcga ttacaccaac
     2701 ttcgaagatc tccagcagca ttatggcgag caccacccag aagccatgaa tgtactcaac
     2761 tttgatcact cggacctgat ctaccggtgt cggttttgtt catacacgag cccgaatgtt
     2821 agaagcctga tgccacatta ccaaagaatg catcccacgg tgaagatcaa caacgcgatg
     2881 atattttcaa gctatgtcgt ggagcagcag gaagggctga atacagaatc ccagaccctg
     2941 agggagattc tgaattcggc tcccaagaac atggcgactt ccacacctgt ggctcgtggt
     3001 ggtggtttgc cagctacgtt caacaaaaac actcctaaga cctttactcc tgaatgtgaa
     3061 aatcagaagg accctttggt caacactgtt gttgtttatg attgtgatgt ttgttcgttt
     3121 gcaagcccca acatgcattc tgtcttggtt cattatcaga agaaacaccc cgaagaaaag
     3181 gcttcctact ttaggatcca gaaaactatg cgaatggtgt ctgtggacag gggctctgcc
     3241 ctttctcaat tatcatttga ggtgggtgct ccaatgtctc ccaaaatgtc caacatgggt
     3301 tccccacccc ccccacaacc cccgccacca gacctcagta ctgagcttta ctactgcaaa
     3361 cactgttcct acagcaatcg gtcagttgtg ggagtgcttg tccactacca gaaaagacac
     3421 ccagaaataa aggttactgc caaatatatc agacaggctc ctcccacagc tgcaatgatg
     3481 agaggggtcg aagggcccca aggctccccc cggccacccg cccccataca acagctgaac
     3541 cgaagcagct ctgagagaga tggccctcct gtggagaatg agatgttctt ttgccagcac
     3601 tgtgattatg ggaaccggac ggtcaaaggg gtactcattc attatcagaa gaagcaccga
     3661 gacttcaagg ccaatgcaga tgtgatccgg cagcatacgg ccaccattcg aagcctctgc
     3721 gaccgaaatc agaagaagcc tgccagctgc gtgcttgtct ccccctctaa tctggagcgg
     3781 gacaaaacga aactccgagc actcaaatgt aggcagtgct catatacctc cccctacttc
     3841 tatgcactga ggaagcatat caagaaagac caccccgccc tgaaagccac agtcacgtcc
     3901 atcatgcgat gggcatttct agatggcttg atagaagctg gctaccactg cgagtggtgc
     3961 atctactccc atacggagcc caacggtttg ctcctgcatt accaacggag gcatccagaa
     4021 cactatgttg attacaccta catggctact aaactgtggg ctgggccaga cccatcccct
     4081 ccctctctca caatgccagc cgaagccaaa acctacagat gcagggactg tgttttcgaa
     4141 gctgtttcca tctgggacat cactaatcac taccaagcat tccacccctg ggccatgaat
     4201 ggtgatgagt cagtgctact ggacatcatc aaggagaaag atgctgtgga gaagcccatt
     4261 ctttcatccg aagagttggc aggccctgtg aattgtgaaa acagtatacc cacccctttc
     4321 ccggagcagg aagctgaatg tccagaggat gcaagactgt cccctgagaa aagcctgcag
     4381 ctagcttcag ccaaccccgc catatcctcc accccatacc agtgcacggt atgccaatct
     4441 gagtataaca acttgcacgg ccttctcact cattatggga agaagcaccc tggcatgaaa
     4501 gtgaaggctg ctgactttgc ccaggacatt gacatcaacc caggtgccgt ctacaaatgc
     4561 aggcattgcc catacatcaa cacccgcatc cacggcgtac tgacccacta ccagaagcga
     4621 cacccgtcca tcaaggtgac cgctgaggac tttgtgcacg acgtagagca gtctgctgac
     4681 atatcccaga atgacgtgga ggagacgagc aggatcttca agcaagggta tggcgcctac
     4741 cggtgcaaac tgtgtccgta cacacacggc actttggaga aactaaaaat ccactacgag
     4801 aagtatcaca atcagcctga atttgatgtc ttttcccagt cgcccccgaa gctgccagtc
     4861 cccctcgagc ccgagatgac cactgaagtg agcccttccc aagtctccat cactgaggag
     4921 gaggtgggag aggagcccgt gtccacttct cacttctcta cctcccacct ggtctcccac
     4981 actgtgttcc ggtgccagct ctgcaagtac ttctgctcca cgaggaaggg gatcgccagg
     5041 cactaccgca tcaagcacaa taatgtccga gcccagccag aaggcaagaa caacctcttc
     5101 aagtgtgccc tgtgtgccta caccaacccc atccgcaaag gtctggcagc ccactaccag
     5161 aagcgccacg acattgatgc gtattacact cactgcttgg cagcctccag gaccatcagc
     5221 gacaagccca acaaagtgat catcccatcc ccgcccaagg acgactcccc tcagctgagc
     5281 gaggaactcc ggcgggcagt ggagaagaaa aagtgctcct tgtgctcttt ccagtcgttc
     5341 agcaagaagg gcatcgtgtc ccattacatg aaacgccacc caggggtgtt cccaaagaag
     5401 cagcacgcca gcaagttggg gggctacttc acggccgtct atgcagatga gcatgagaag
     5461 cccacactga tggaagaaga ggagagaggc aactttgaga aagccgaggt ggagggtgaa
     5521 gctcaggaaa tcgagtggct cccattccgc tgcatcaaat gcttcaagct gtcctttagc
     5581 actgcagagc tgctgtgcat gcattacact gaccaccaca gtcgggacct aaagagggac
     5641 ttcatcattc tgggcaacgg cccccgcttg cagaactcca cctaccagtg taagcactgt
     5701 gatagcaaac tgcaaagcac agccgagctg acctcacact tgaacattca caatgaggaa
     5761 ttccagaagc gtgccaaacg tcaggagagg aggaaacagc ttttgagcaa gcagaaatat
     5821 gcagatggtg cttttgcaga tttcaaacaa gagaggcctt ttggtcactt agaagaggtg
     5881 ccaaagatca aggagaggaa agtggtgggc tacaaatgta aattctgtgt ggaagtgcac
     5941 ccaacgctcc gagccatctg caatcacctc cgaaagcacg tccagtatgg caatgtccca
     6001 gctgtgtcag ctgctgtgaa ggggctgcgt tctcatgaga ggagccacct ggccctggcc
     6061 atgtttaccc gcgaggacaa gtacagctgc cagtattgct cgtttgtttc tgctttcagg
     6121 cacaatttgg atcgccatat gcaaacccac cacggacacc ataaaccatt ccgatgcaaa
     6181 ctctgctcct tcaagtcctc ctataacagc cggctgaaaa cacatatact caaagctcat
     6241 gctggtgagc atgcctacaa gtgttcttgg tgctcattct ccaccatgac aatcagccag
     6301 ctgaaggaac actccctcaa ggtccacgga aaagccctga ccctccccag gccacggatc
     6361 gtcagtctcc tctcctcaca ctcccaccac tcctcccaaa aagctacccc ggctgaagaa
     6421 gtggaagact ccaatgactc atcatattca gagcccccag atgttcagca gcagttgaac
     6481 cactatcagt cagctgccct ggcaaggaac aacagccgtg ttagccctgt gcctctttct
     6541 ggggctgctg ctggcactga gcagaaaact gaagccgtgc ttcactgcga attctgtgaa
     6601 ttctcctccg gctacatcca gagcatcagg cgtcattacc gggacaagca tggtgggaag
     6661 aagcttttca agtgcaaaga ctgctccttt tacacaggct ttaaatctgc ttttactatg
     6721 cacgtggaag ctgggcactc agcagttccc gaggagggcc ccaaagatct tcgctgtcct
     6781 ctctgcctct atcacaccaa atacaagcgc aacatgattg accacatcgt gctgcaccga
     6841 gaagagcgtg ttgtccccat tgaagtttgc cggtccaaac tgtccaaata cttgcaggga
     6901 gtagttttcc gctgtgataa gtgtaccttc acctgctcca gtgatgagag cctccagcaa
     6961 catatagaaa agcacaatga actgaaacct tacaaatgcc agctctgcta ctatgagacc
     7021 aagcacacgg aggaactgga cagccacctt cgggatgagc ataaggtaag ccgtaacttt
     7081 gagctggttg gacgggttaa cttggatcag ctggaacaga tgaaggagaa aatggagagc
     7141 tccagcagcg atgatgagga caaggaagaa gaaatgaaca gcaaggctga agacagagag
     7201 ctgatgagat tttctgacca cggggctgct cttaacactg agaagcgttt tccatgtgaa
     7261 ttttgtggac gggcgttttc acagggctct gagtgggaaa gacatgtgct gagacacggc
     7321 atggcattga atgacaccaa gcaggtgagc agagaagaaa tccacccaaa agagatcatg
     7381 gagaacagtg ttaaaatgcc ctccatagag gaaaaggaag atgacgaggc cattgggata
     7441 gacttttccc taaagaatga aacagtagcc atctgtgtag taactgccga caaatctctc
     7501 ctggagaatg cagaggccaa aaaagaatag taatgaacta gtccgtaact tgaaagtatt
     7561 tcgatttctt ggctttatat atcttgtgga aaggacgagg attgctagct cgctatgccg
     7621 gg
//
