LOCUS       sequence-284193-        6971 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6971)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 6971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2428
FEATURES             Location/Qualifiers
     source          1..6971
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             259..276
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             280..297
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(1258..1269)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 accgccacca tggcccataa cgcgggcgcc gcggccgccg ccggcaccca cagcgccaag
       61 agcggcggct ccgaggcggc tctcaaggag ggtggaagcg ccgccgcgct gtcctcctcc
      121 tcctcctcct ccgcggcggc agcggcggca tcctcttcct cctcgtcggg cccgggctcg
      181 gccatggaga cggggctgct ccccaaccac aaactgaaaa ccgttggcga agcccccgcc
      241 gcgccgcccc accagcagca ccaccaccac caccatgccc accaccacca ccaccatgcc
      301 caccacctcc accaccacca cgcactacag cagcagctaa accagttcca gcagcagcag
      361 cagcagcagc aacagcagca gcagcagcag cagcaacagc aacatcccat ttccaacaac
      421 aacagcttgg gcggcgcggg cggcggcgcg cctcagcccg gccccgacat ggagcagccg
      481 caacatggag gcgccaagga cagtgctgcg ggcggccagg ccgacccccc gggcccgccg
      541 ctgctgagca agccgggcga cgaggacgac gcgccgccca agatggggga gccggcgggc
      601 ggccgctacg agcacccggg cttgggcgcc ctgggcacgc agcagccgcc ggtcgccgtg
      661 cccgggggcg gcggcggccc ggcggccgtc ccggagttta ataattacta tggcagcgct
      721 gcccctgcga gcggcggccc cggcggccgc gctgggcctt gctttgatca acatggcgga
      781 caacaaagcc ccgggatggg gatgatgcac tccgcctccg ccgccgccgc cggggccccc
      841 ggcagcatgg accccctgca gaactcccac gaagggtacc ccaacagcca gtgcaaccat
      901 tatccgggct acagccggcc cggcgcgggc ggcggcggcg gcggcggcgg cggaggagga
      961 ggaggcagcg gaggaggagg aggaggagga ggagcaggag caggaggagc aggagcggga
     1021 gctgtggcgg cggcggccgc ggcggcggcg gcagcagcag gaggcggcgg cggcggcggc
     1081 tatgggggct cgtccgcggg gtacggggtg ctgagctccc cccggcagca gggcggcggc
     1141 atgatgatgg gccccggggg cggcggggcc gcgagcctca gcaaggcggc cgccggctcg
     1201 gcggcggggg gcttccagcg cttcgccggc cagaaccagc acccgtcggg ggccaccccg
     1261 accctcaatc agctgctcac ctcgcccagc cccatgatgc ggagctacgg cggcagctac
     1321 cccgagtaca gcagccccag cgcgccgccg ccgccgccgt cgcagcccca gtcccaggcg
     1381 gcggcggcgg gggcggcggc gggcggccag caggcggccg cgggcatggg cttgggcaag
     1441 gacatgggcg cccagtacgc cgctgccagc ccggcctggg cggccgcgca acaaaggagt
     1501 cacccggcga tgagccccgg cacccccgga ccgaccatgg gcagatccca gggcagccca
     1561 atggatccaa tggtgatgaa gagacctcag ttgtatggca tgggcagtaa ccctcattct
     1621 cagcctcagc agagcagtcc gtacccagga ggttcctatg gccctccagg cccacagcgg
     1681 tatccaattg gcatccaggg tcggactccc ggggccatgg ccggaatgca gtaccctcag
     1741 cagcagatgc cacctcagta tggacagcaa ggtgtgagtg gttactgcca gcagggccaa
     1801 cagccatatt acagccagca gccgcagccc ccgcacctcc caccccaggc gcagtatctg
     1861 ccgtcccagt cccagcagag gtaccagccg cagcaggaca tgtctcagga aggctatgga
     1921 actagatctc aacctcctct ggcccccgga aaacctaacc atgaagactt gaacttaata
     1981 cagcaagaaa gaccatcaag tttaccagat ctgtctggct ccattgatga cctccccacg
     2041 ggaacggaag caactttgag ctcagcagtc agtgcatccg ggtccacgag cagccaaggg
     2101 gatcagagca acccggcgca gtcgcctttc tccccacatg cgtcccctca tctctccagc
     2161 atcccggggg gcccatctcc ctctcctgtt ggctctcctg taggaagcaa ccagtctcga
     2221 tctggcccaa tctctcctgc aagtatccca ggtagtcaga tgcctccgca gccacccggg
     2281 agccagtcag aatccagttc ccatcccgcc ttgagccagt caccaatgcc acaggaaaga
     2341 ggttttatgg caggcacaca aagaaaccct cagatggctc agtatggacc tcaacagaca
     2401 ggaccatcca tgtcgcctca tccttctcct gggggccaga tgcatgctgg aatcagtagc
     2461 tttcagcaga gtaactcaag tgggacttac ggtccacaga tgagccagta tggaccacaa
     2521 ggtaactact ccagaccccc agcgtatagt ggggtgccca gtgcaagcta cagcggccca
     2581 gggcccggta tgggtatcag tgccaacaac cagatgcatg gacaagggcc aagccagcca
     2641 tgtggtgctg tgcccctggg acgaatgcca tcagctggga tgcagaacag accatttcct
     2701 ggaaatatga gcagcatgac ccccagttct cctggcatgt ctcagcaggg agggccagga
     2761 atggggccgc caatgccaac tgtgaaccgt aaggcacagg aggcagccgc agcagtgatg
     2821 caggctgctg cgaactcagc acaaagcagg caaggcagtt tccccggcat gaaccagagt
     2881 ggacttatgg cttccagctc tccctacagc cagcccatga acaacagctc tagcctgatg
     2941 aacacgcagg cgccgcccta cagcatggcg cccgccatgg tgaacagctc ggcagcatct
     3001 gtgggtcttg cagatatgat gtctcctggt gaatccaaac tgcccctgcc tctcaaagca
     3061 gacggcaaag aagaaggcac tccacagccc gagagcaagt caaaggatag ctacagctct
     3121 cagggtattt ctcagccccc aaccccaggc aacctgccag tcccttcccc aatgtccccc
     3181 agctctgcta gcatctcctc atttcatgga gatgaaagtg atagcattag cagcccaggc
     3241 tggccaaaga ctccatcaag ccctaagtcc agctcctcca ccactactgg ggagaagatc
     3301 acgaaggtgt acgagctggg gaatgagcca gagagaaagc tctgggtcga ccgatacctc
     3361 accttcatgg aagagagagg ctctcctgtc tcaagtctgc ctgccgtggg caagaagccc
     3421 ctggacctgt tccgactcta cgtctgcgtc aaagagatcg ggggtttggc ccaggttaat
     3481 aaaaacaaga agtggcgtga gctggcaacc aacctaaacg ttggcacctc aagcagtgca
     3541 gcgagctccc tgaaaaagca gtatattcag tacctgtttg cctttgagtg caagatcgaa
     3601 cgtggggagg agcccccgcc ggaagtcttc agcaccgggg acaccaaaaa gcagcccaag
     3661 ctccagccgc catctcctgc taactcggga tccttgcaag gcccacagac cccccagtca
     3721 actggcagca attccatggc agaggttcca ggtgacctga agccacctac cccagcctcc
     3781 acccctcacg gccagatgac tccaatgcaa ggtggaagaa gcagtacaat cagtgtgcac
     3841 gacccattct cagatgtgag tgattcatcc ttcccgaaac ggaactccat gactccaaac
     3901 gccccctacc agcagggcat gagcatgccc gatgtgatgg gcaggatgcc ctatgagccc
     3961 aacaaggacc cctttggggg aatgagaaaa gtgcctggaa gcagcgagcc ctttatgacg
     4021 caaggacaga tgcccaacag cagcatgcag gacatgtaca accaaagtcc ctccggagca
     4081 atgtctaacc tgggcatggg gcagcgccag cagtttccct atggagccag ttacgaccga
     4141 aggcatgaac cttatgggca gcagtatcca ggccaaggcc ctccctcggg acagccgccg
     4201 tatggagggc accagcccgg cctgtaccca cagcagccga attacaaacg ccatatggac
     4261 ggcatgtacg ggcccccagc caagcgccac gagggcgaca tgtacaacat gcagtacagc
     4321 agccagcagc aggagatgta caaccagtat ggaggctcct actcgggccc ggaccgcagg
     4381 cccatccagg gccagtaccc gtatccctac agcagggaga ggatgcaggg cccggggcag
     4441 atccagacac acggaatccc gcctcagatg atgggcggcc cgctgcagtc gtcctccagt
     4501 gaggggcctc agcagaatat gtgggcagca cgcaatgata tgccttatcc ctaccagaac
     4561 aggcagggcc ctggcggccc tacacaggcg cccccttacc caggcatgaa ccgcacagac
     4621 gatatgatgg tacccgatca gaggataaat catgagagcc agtggccttc tcacgtcagc
     4681 cagcgtcagc cttatatgtc gtcctcagcc tccatgcagc ccatcacacg cccaccacag
     4741 ccgtcctacc agacgccacc gtcactgcca aatcacatct ccagggcgcc cagcccagcg
     4801 tccttccagc gctccctgga gaaccgcatg tctccaagca agtctccttt tctgccgtct
     4861 atgaagatgc agaaggtcat gcccacggtc cccacatccc aggtcaccgg gccaccaccc
     4921 caaccacccc caatcagaag ggagatcacc tttcctcctg gctcagtaga agcatcacaa
     4981 ccagtcttga aacaaaggcg aaagattacc tccaaagata tcgttactcc tgaggcgtgg
     5041 cgtgtgatga tgtcccttaa atcaggtctt ttggctgaga gtacgtgggc tttggacact
     5101 attaatattc ttctgtatga tgacagcact gttgctactt tcaatctctc ccagttgtct
     5161 ggatttctcg aacttttagt cgagtacttt agaaaatgcc tgattgacat ttttggaatt
     5221 cttatggaat atgaagtggg agaccccagc caaaaagcac ttgatcacaa cgcagcaagg
     5281 aaggatgaca gccagtcctt ggcagacgat tctgggaaag aggaggaaga tgctgaatgt
     5341 attgatgacg acgaggaaga cgaggaggat gaggaggaag acagcgagaa gacagaaagc
     5401 gatgaaaaga gcagcatcgc tctgactgcc ccggacgccg ctgcagaccc aaaggagaag
     5461 cccaagcaag ccagtaagtt cgacaagctg ccaataaaga tagtcaaaaa gaacaacctg
     5521 tttgttgttg accgatctga caagttgggg cgtgtgcagg agttcaatag tggccttctg
     5581 cactggcagc tcggcggggg tgacaccacc gagcacattc agactcactt tgagagcaag
     5641 atggaaattc ctcctcgcag gcgcccacct ccccccttaa gctccgcagg tagaaagaaa
     5701 gagcaagaag gcaaaggcga ctctgaagag cagcaagaga aaagcatcat agcaaccatc
     5761 gatgacgtcc tctctgctcg gccaggggca ttgcctgaag acgcaaaccc tgggccccag
     5821 accgaaagca gtaagtttcc ctttggtatc cagcaagcca aaagtcaccg gaacatcaag
     5881 ctgctggagg acgagcccag gagccgagac gagactcctc tgtgtaccat cgcgcactgg
     5941 caggactcgc tggctaagcg atgcatctgt gtgtccaata ttgtccgtag cttgtcattc
     6001 gtgcctggca atgatgccga aatgtccaaa catccaggcc tggtgctgat cctggggaag
     6061 ctgattcttc ttcaccacga gcatccagag agaaagcgag caccgcagac ctatgagaaa
     6121 gaggaggatg aggacaaggg ggtggcctgc agcaaagatg agtggtggtg ggactgcctc
     6181 gaggtcttga gggataacac gttggtcacg ttggccaaca tttccgggca gctagacttg
     6241 tctgcttaca cggaaagcat ctgcttgcca attttggatg gcttgctgca ctggatggtg
     6301 tgcccgtctg cagaggcaca agatcccttt ccaactgtgg gacccaactc ggtcctgtcg
     6361 cctcagagac ttgtgctgga gaccctctgt aaactcagta tccaggacaa taatgtggac
     6421 ctgatcttgg ccactcctcc atttagtcgt caggagaaat tctatgctac attagttagg
     6481 tacgttgggg atcgcaaaaa cccagtctgt cgagaaatgt ccatggcgct tttatcgaac
     6541 cttgcccaag gggacgcact agcagcaagg gccatagctg tgcagaaagg aagcattgga
     6601 aacttgataa gcttcctaga ggatggggtc acgatggccc agtaccagca gagccagcac
     6661 aacctcatgc acatgcagcc cccgcccctg gaaccaccta gcgtagacat gatgtgcagg
     6721 gcggccaagg ctttgctagc catggccaga gtggacgaaa accgctcgga attccttttg
     6781 cacgagggcc ggttgctgga tatctcgata tcagctgtcc tgaactctct ggttgcatct
     6841 gtcatctgtg atgtactgtt tcagattggg cagttatagt aatgaactag tccgtaactt
     6901 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgagcc atgtggttga
     6961 agcagtctat t
//
