LOCUS       sequence-284199-        6851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6851)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 6851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2430
FEATURES             Location/Qualifiers
     source          1..6851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             259..276
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             280..297
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(1258..1269)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 accgccacca tggcccataa cgcgggcgcc gcggccgccg ccggcaccca cagcgccaag
       61 agcggcggct ccgaggcggc tctcaaggag ggtggaagcg ccgccgcgct gtcctcctcc
      121 tcctcctcct ccgcggcggc agcggcggca tcctcttcct cctcgtcggg cccgggctcg
      181 gccatggaga cggggctgct ccccaaccac aaactgaaaa ccgttggcga agcccccgcc
      241 gcgccgcccc accagcagca ccaccaccac caccatgccc accaccacca ccaccatgcc
      301 caccacctcc accaccacca cgcactacag cagcagctaa accagttcca gcagcagcag
      361 cagcagcagc aacagcagca gcagcagcag cagcaacagc aacatcccat ttccaacaac
      421 aacagcttgg gcggcgcggg cggcggcgcg cctcagcccg gccccgacat ggagcagccg
      481 caacatggag gcgccaagga cagtgctgcg ggcggccagg ccgacccccc gggcccgccg
      541 ctgctgagca agccgggcga cgaggacgac gcgccgccca agatggggga gccggcgggc
      601 ggccgctacg agcacccggg cttgggcgcc ctgggcacgc agcagccgcc ggtcgccgtg
      661 cccgggggcg gcggcggccc ggcggccgtc ccggagttta ataattacta tggcagcgct
      721 gcccctgcga gcggcggccc cggcggccgc gctgggcctt gctttgatca acatggcgga
      781 caacaaagcc ccgggatggg gatgatgcac tccgcctccg ccgccgccgc cggggccccc
      841 ggcagcatgg accccctgca gaactcccac gaagggtacc ccaacagcca gtgcaaccat
      901 tatccgggct acagccggcc cggcgcgggc ggcggcggcg gcggcggcgg cggaggagga
      961 ggaggcagcg gaggaggagg aggaggagga ggagcaggag caggaggagc aggagcggga
     1021 gctgtggcgg cggcggccgc ggcggcggcg gcagcagcag gaggcggcgg cggcggcggc
     1081 tatgggggct cgtccgcggg gtacggggtg ctgagctccc cccggcagca gggcggcggc
     1141 atgatgatgg gccccggggg cggcggggcc gcgagcctca gcaaggcggc cgccggctcg
     1201 gcggcggggg gcttccagcg cttcgccggc cagaaccagc acccgtcggg ggccaccccg
     1261 accctcaatc agctgctcac ctcgcccagc cccatgatgc ggagctacgg cggcagctac
     1321 cccgagtaca gcagccccag cgcgccgccg ccgccgccgt cgcagcccca gtcccaggcg
     1381 gcggcggcgg gggcggcggc gggcggccag caggcggccg cgggcatggg cttgggcaag
     1441 gacatgggcg cccagtacgc cgctgccagc ccggcctggg cggccgcgca acaaaggagt
     1501 cacccggcga tgagccccgg cacccccgga ccgaccatgg gcagatccca gggcagccca
     1561 atggatccaa tggtgatgaa gagacctcag ttgtatggca tgggcagtaa ccctcattct
     1621 cagcctcagc agagcagtcc gtacccagga ggttcctatg gccctccagg cccacagcgg
     1681 tatccaattg gcatccaggg tcggactccc ggggccatgg ccggaatgca gtaccctcag
     1741 cagcaggact ctggagatgc cacatggaaa gaaacattct ggttgatgcc acctcagtat
     1801 ggacagcaag gtgtgagtgg ttactgccag cagggccaac agccatatta cagccagcag
     1861 ccgcagcccc cgcacctccc accccaggcg cagtatctgc cgtcccagtc ccagcagagg
     1921 taccagccgc agcaggacat gtctcaggaa ggctatggaa ctagatctca acctcctctg
     1981 gcccccggaa aacctaacca tgaagacttg aacttaatac agcaagaaag accatcaagt
     2041 ttaccagatc tgtctggctc cattgatgac ctccccacgg gaacggaagc aactttgagc
     2101 tcagcagtca gtgcatccgg gtccacgagc agccaagggg atcagagcaa cccggcgcag
     2161 tcgcctttct ccccacatgc gtcccctcat ctctccagca tcccgggggg cccatctccc
     2221 tctcctgttg gctctcctgt aggaagcaac cagtctcgat ctggcccaat ctctcctgca
     2281 agtatcccag gtagtcagat gcctccgcag ccacccggga gccagtcaga atccagttcc
     2341 catcccgcct tgagccagtc accaatgcca caggaaagag gttttatggc aggcacacaa
     2401 agaaaccctc agatggctca gtatggacct caacagacag gaccatccat gtcgcctcat
     2461 ccttctcctg ggggccagat gcatgctgga atcagtagct ttcagcagag taactcaagt
     2521 gggacttacg gtccacagat gagccagtat ggaccacaag gtaactactc cagaccccca
     2581 gcgtatagtg gggtgcccag tgcaagctac agcggcccag ggcccggtat gggtatcagt
     2641 gccaacaacc agatgcatgg acaagggcca agccagccat gtggtgctgt gcccctggga
     2701 cgaatgccat cagctgggat gcagaacaga ccatttcctg gaaatatgag cagcatgacc
     2761 cccagttctc ctggcatgtc tcagcaggga gggccaggaa tggggccgcc aatgccaact
     2821 gtgaaccgta aggcacagga ggcagccgca gcagtgatgc aggctgctgc gaactcagca
     2881 caaagcaggc aaggcagttt ccccggcatg aaccagagtg gacttatggc ttccagctct
     2941 ccctacagcc agcccatgaa caacagctct agcctgatga acacgcaggc gccgccctac
     3001 agcatggcgc ccgccatggt gaacagctcg gcagcatctg tgggtcttgc agatatgatg
     3061 tctcctggtg aatccaaact gcccctgcct ctcaaagcag acggcaaaga agaaggcact
     3121 ccacagcccg agagcaagtc aaagaagtcc agctcctcca ccactactgg ggagaagatc
     3181 acgaaggtgt acgagctggg gaatgagcca gagagaaagc tctgggtcga ccgatacctc
     3241 accttcatgg aagagagagg ctctcctgtc tcaagtctgc ctgccgtggg caagaagccc
     3301 ctggacctgt tccgactcta cgtctgcgtc aaagagatcg ggggtttggc ccaggttaat
     3361 aaaaacaaga agtggcgtga gctggcaacc aacctaaacg ttggcacctc aagcagtgca
     3421 gcgagctccc tgaaaaagca gtatattcag tacctgtttg cctttgagtg caagatcgaa
     3481 cgtggggagg agcccccgcc ggaagtcttc agcaccgggg acaccaaaaa gcagcccaag
     3541 ctccagccgc catctcctgc taactcggga tccttgcaag gcccacagac cccccagtca
     3601 actggcagca attccatggc agaggttcca ggtgacctga agccacctac cccagcctcc
     3661 acccctcacg gccagatgac tccaatgcaa ggtggaagaa gcagtacaat cagtgtgcac
     3721 gacccattct cagatgtgag tgattcatcc ttcccgaaac ggaactccat gactccaaac
     3781 gccccctacc agcagggcat gagcatgccc gatgtgatgg gcaggatgcc ctatgagccc
     3841 aacaaggacc cctttggggg aatgagaaaa gtgcctggaa gcagcgagcc ctttatgacg
     3901 caaggacaga tgcccaacag cagcatgcag gacatgtaca accaaagtcc ctccggagca
     3961 atgtctaacc tgggcatggg gcagcgccag cagtttccct atggagccag ttacgaccga
     4021 aggcatgaac cttatgggca gcagtatcca ggccaaggcc ctccctcggg acagccgccg
     4081 tatggagggc accagcccgg cctgtaccca cagcagccga attacaaacg ccatatggac
     4141 ggcatgtacg ggcccccagc caagcgccac gagggcgaca tgtacaacat gcagtacagc
     4201 agccagcagc aggagatgta caaccagtat ggaggctcct actcgggccc ggaccgcagg
     4261 cccatccagg gccagtaccc gtatccctac agcagggaga ggatgcaggg cccggggcag
     4321 atccagacac acggaatccc gcctcagatg atgggcggcc cgctgcagtc gtcctccagt
     4381 gaggggcctc agcagaatat gtgggcagca cgcaatgata tgccttatcc ctaccagaac
     4441 aggcagggcc ctggcggccc tacacaggcg cccccttacc caggcatgaa ccgcacagac
     4501 gatatgatgg tacccgatca gaggataaat catgagagcc agtggccttc tcacgtcagc
     4561 cagcgtcagc cttatatgtc gtcctcagcc tccatgcagc ccatcacacg cccaccacag
     4621 ccgtcctacc agacgccacc gtcactgcca aatcacatct ccagggcgcc cagcccagcg
     4681 tccttccagc gctccctgga gaaccgcatg tctccaagca agtctccttt tctgccgtct
     4741 atgaagatgc agaaggtcat gcccacggtc cccacatccc aggtcaccgg gccaccaccc
     4801 caaccacccc caatcagaag ggagatcacc tttcctcctg gctcagtaga agcatcacaa
     4861 ccagtcttga aacaaaggcg aaagattacc tccaaagata tcgttactcc tgaggcgtgg
     4921 cgtgtgatga tgtcccttaa atcaggtctt ttggctgaga gtacgtgggc tttggacact
     4981 attaatattc ttctgtatga tgacagcact gttgctactt tcaatctctc ccagttgtct
     5041 ggatttctcg aacttttagt cgagtacttt agaaaatgcc tgattgacat ttttggaatt
     5101 cttatggaat atgaagtggg agaccccagc caaaaagcac ttgatcacaa cgcagcaagg
     5161 aaggatgaca gccagtcctt ggcagacgat tctgggaaag aggaggaaga tgctgaatgt
     5221 attgatgacg acgaggaaga cgaggaggat gaggaggaag acagcgagaa gacagaaagc
     5281 gatgaaaaga gcagcatcgc tctgactgcc ccggacgccg ctgcagaccc aaaggagaag
     5341 cccaagcaag ccagtaagtt cgacaagctg ccaataaaga tagtcaaaaa gaacaacctg
     5401 tttgttgttg accgatctga caagttgggg cgtgtgcagg agttcaatag tggccttctg
     5461 cactggcagc tcggcggggg tgacaccacc gagcacattc agactcactt tgagagcaag
     5521 atggaaattc ctcctcgcag gcgcccacct ccccccttaa gctccgcagg tagaaagaaa
     5581 gagcaagaag gcaaaggcga ctctgaagag cagcaagaga aaagcatcat agcaaccatc
     5641 gatgacgtcc tctctgctcg gccaggggca ttgcctgaag acgcaaaccc tgggccccag
     5701 accgaaagca gtaagtttcc ctttggtatc cagcaagcca aaagtcaccg gaacatcaag
     5761 ctgctggagg acgagcccag gagccgagac gagactcctc tgtgtaccat cgcgcactgg
     5821 caggactcgc tggctaagcg atgcatctgt gtgtccaata ttgtccgtag cttgtcattc
     5881 gtgcctggca atgatgccga aatgtccaaa catccaggcc tggtgctgat cctggggaag
     5941 ctgattcttc ttcaccacga gcatccagag agaaagcgag caccgcagac ctatgagaaa
     6001 gaggaggatg aggacaaggg ggtggcctgc agcaaagatg agtggtggtg ggactgcctc
     6061 gaggtcttga gggataacac gttggtcacg ttggccaaca tttccgggca gctagacttg
     6121 tctgcttaca cggaaagcat ctgcttgcca attttggatg gcttgctgca ctggatggtg
     6181 tgcccgtctg cagaggcaca agatcccttt ccaactgtgg gacccaactc ggtcctgtcg
     6241 cctcagagac ttgtgctgga gaccctctgt aaactcagta tccaggacaa taatgtggac
     6301 ctgatcttgg ccactcctcc atttagtcgt caggagaaat tctatgctac attagttagg
     6361 tacgttgggg atcgcaaaaa cccagtctgt cgagaaatgt ccatggcgct tttatcgaac
     6421 cttgcccaag gggacgcact agcagcaagg gccatagctg tgcagaaagg aagcattgga
     6481 aacttgataa gcttcctaga ggatggggtc acgatggccc agtaccagca gagccagcac
     6541 aacctcatgc acatgcagcc cccgcccctg gaaccaccta gcgtagacat gatgtgcagg
     6601 gcggccaagg ctttgctagc catggccaga gtggacgaaa accgctcgga attccttttg
     6661 cacgagggcc ggttgctgga tatctcgata tcagctgtcc tgaactctct ggttgcatct
     6721 gtcatctgtg atgtactgtt tcagattggg cagttatagt aatgaactag tccgtaactt
     6781 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgacga ccaaagggcc
     6841 acgtccggcc c
//
