LOCUS       sequence-284205-        6308 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6308)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 6308)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2433
FEATURES             Location/Qualifiers
     source          1..6308
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     RBS             2790..2798
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 accgccacca tggccgcgca ggtcgccccc gccgccgcca gcagcctggg caacccgccg
       61 ccgccgccgc cctcggagct gaagaaagcc gagcagcagc agcgggagga ggcggggggc
      121 gaggcggcgg cggcggcagc ggccgagcgc ggggaaatga aggcagccgc cgggcaggaa
      181 agcgagggcc ccgccgtggg gccgccgcag ccgctgggaa aggagctgca ggacggggcc
      241 gagagcaatg ggggtggcgg cggcggcgga gccggcagcg gcggcgggcc cggcgcggag
      301 ccggacctga agaactcgaa cgggaacgcg ggccctaggc ccgccctgaa caataacctc
      361 acggagccgc ccggcggcgg cggtggcggc agcagcgatg gggtgggggc gcctcctcac
      421 tcagccgcgg ccgccttgcc gcccccagcc tacggcttcg ggcaacccta cggccggagc
      481 ccgtctgccg tcgccgccgc cgcggccgcc gtcttccacc aacaacatgg cggacaacaa
      541 agccctggcc tggcagcgct gcagagcggc ggcggcgggg gcctggagcc ctacgcgggg
      601 ccccagcaga actctcacga ccacggcttc cccaaccacc agtacaactc ctactacccc
      661 aaccgcagcg cctacccccc gcccgccccg gcctacgcgc tgagctcccc gagaggtggc
      721 actccgggct ccggcgcggc ggcggctgcc ggctccaagc cgcctccctc ctccagcgcc
      781 tccgcctcct cgtcgtcttc gtccttcgct cagcagcgct tcggggccat ggggggaggc
      841 ggcccctccg cggccggcgg gggaactccc cagcccaccg ccacccccac cctcaaccaa
      901 ctgctcacgt cgcccagctc ggcccggggc taccagggct accccggggg cgactacagt
      961 ggcgggcccc aggacggggg cgccggcaag ggcccggcgg acatggcctc gcagtgttgg
     1021 ggggctgcgg cggcggcagc tgcggcggcg gccgcctcgg gaggggccca acaaaggagc
     1081 caccacgcgc ccatgagccc cgggagcagc ggcggcgggg ggcagccgct cgcccggacc
     1141 cctcagccat ccagtccaat ggatcagatg ggcaagatga gacctcagcc atatggcggg
     1201 actaacccat actcgcagca acagggacct ccgtcaggac cgcagcaagg acatgggtac
     1261 ccagggcagc catacgggtc ccagaccccg cagcggtacc cgatgaccat gcagggccgg
     1321 gcgcagagtg ccatgggcgg cctctcttat acacagcaga ttcctcctta tggacaacaa
     1381 ggccccagcg ggtatggtca acagggccag actccatatt acaaccagca aagtcctcac
     1441 cctcagcagc agcagccacc ctactcccag caaccaccgt cccagacccc tcatgcccaa
     1501 ccttcgtatc agcagcagcc acagtctcaa ccaccacagc tccagtcctc tcagcctcca
     1561 tactcccagc agccatccca gcctccacat cagcagtccc cggctccata cccctcccag
     1621 cagtcgacga cacagcagca cccccagagc cagcccccct actcacagcc acaggctcag
     1681 tctccttacc agcagcagca acctcagcag ccagcaccct cgacgctctc ccagcaggct
     1741 gcgtatcctc agccccagtc tcagcagtcc cagcaaactg cctattccca gcagcgcttc
     1801 cctccaccgc aggagctatc tcaagattca tttgggtctc aggcatcctc agccccctca
     1861 atgacctcca gtaagggagg gcaagaagat atgaacctga gccttcagtc aagaccctcc
     1921 agcttgcctg atctatctgg ttcaatagat gacctcccca tggggacaga aggagctctg
     1981 agtcctggag tgagcacatc agggatttcc agcagccaag gagagcagag taatccagct
     2041 cagtctcctt tctctcctca tacctcccct cacctgcctg gcatccgagg cccttccccg
     2101 tcccctgttg gctctcccgc cagtgttgct cagtctcgct caggaccact ctcgcctgct
     2161 gcagtgccag gcaaccagat gccacctcgg ccacccagtg gccagtcgga cagcatcatg
     2221 catccttcca tgaaccaatc aagcattgcc caagatcgag gttatatgca gaggaacccc
     2281 cagatgcccc agtacagttc cccccagccc ggctcagcct tatctccgcg tcagccttcc
     2341 ggaggacaga tacacacagg catgggctcc taccagcaga actccatggg gagctatggt
     2401 ccccaggggg gtcagtatgg cccacaaggt ggctacccca ggcagccaaa ctataatgcc
     2461 ttgcccaatg ccaactaccc cagtgcaggc atggctggag gcataaaccc catgggtgcc
     2521 ggaggtcaaa tgcatggaca gcctggcatc ccaccttatg gcacactccc tccagggagg
     2581 atgagtcacg cctccatggg caaccggcct tatggcccta acatggccaa tatgccacct
     2641 caggttgggt cagggatgtg tcccccacca gggggcatga accggaaaac ccaagaaact
     2701 gctgtcgcca tgcatgttgc tgccaactct atccaaaaca ggccgccagg ctaccccaat
     2761 atgaatcaag ggggcatgat gggaactgga cctccttatg gacaagggat taatagtatg
     2821 gctggcatga tcaaccctca gggaccccca tattccatgg gtggaaccat ggccaacaat
     2881 tctgcaggga tggcagccag cccagagatg atgggccttg gggatgtaaa gttaactcca
     2941 gccaccaaaa tgaacaacaa ggcagatggg acacccaaga cagaatccaa atccaagaaa
     3001 tccagttctt ctactacaac caatgagaag atcaccaagt tgtatgagct gggtggtgag
     3061 cctgagagga agatgtgggt ggaccgttat ctggccttca ctgaggagaa ggccatgggc
     3121 atgacaaatc tgcctgctgt gggtaggaaa cctctggacc tctatcgcct ctatgtgtct
     3181 gtgaaggaga ttggtggatt gactcaggtc aacaagaaca aaaaatggcg ggaacttgca
     3241 accaacctca atgtgggcac atcaagcagt gctgccagct ccttgaaaaa gcagtatatc
     3301 cagtgtctct atgcctttga atgcaagatt gaacggggag aagaccctcc cccagacatc
     3361 tttgcagctg ctgattccaa gaagtcccag cccaagatcc agcctccctc tcctgcggga
     3421 tcaggatcta tgcaggggcc ccagactccc cagtcaacca gcagttccat ggcagaagga
     3481 ggagacttaa agccaccaac tccagcatcc acaccacaca gtcagatccc cccattgcca
     3541 ggcatgagca ggagcaattc agttgggatc caggatgcct ttaatgatgg aagtgactcc
     3601 acattccaga agcggaattc catgactcca aaccctgggt atcagcccag tatgaatacc
     3661 tctgacatga tggggcgcat gtcctatgag ccaaataagg atccttatgg cagcatgagg
     3721 aaagctccag ggagtgatcc cttcatgtcc tcagggcagg gccccaacgg cgggatgggt
     3781 gacccctaca gtcgtgctgc cggccctggg ctaggaaatg tggcgatggg accacgacag
     3841 cactatccct atggaggtcc ttatgacaga gtgaggacgg agcctggaat agggcctgag
     3901 ggaaacatga gcactggggc cccacagccg aatctcatgc cttccaaccc agactcgggg
     3961 atgtattctc ctagccgcta ccccccgcag cagcagcagc agcagcagca acgacatgat
     4021 tcctatggca atcagttctc cacccaaggc accccttctg gcagcccctt ccccagccag
     4081 cagactacaa tgtatcaaca gcaacagcag gtatccagcc ctgctcccct gccccggcca
     4141 atggagaacc gcacctctcc tagcaagtct ccattcctgc actctgggat gaaaatgcag
     4201 aaggcaggtc ccccagtacc tgcctcgcac atagcacctg cccctgtgca gccccccatg
     4261 attcggcggg atatcacctt cccacctggc tctgttgaag ccacacagcc tgtgttgaag
     4321 cagaggaggc ggctcacaat gaaagacatt ggaaccccgg aggcatggcg ggtaatgatg
     4381 tccctcaagt ctggtctcct ggcagagagc acatgggcat tagataccat caacatcctg
     4441 ctgtatgatg acaacagcat catgaccttc aacctcagtc agctcccagg gttgctagag
     4501 ctccttgtag aatatttccg acgatgcctg attgagatct ttggcatttt aaaggagtat
     4561 gaggtgggtg acccaggaca gagaacgcta ctggatcctg ggaggttcag caaggtgtct
     4621 agtccagctc ccatggaggg tggggaagaa gaagaagaac ttctaggtcc taaactagaa
     4681 gaggaagaag aagaggaagt agttgaaaat gatgaggaga tagccttttc aggcaaggac
     4741 aagccagctt cagagaatag tgaggagaag ctgatcagta agtttgacaa gcttccagta
     4801 aagatcgtac agaagaatga tccatttgtg gtggactgct cagataagct tgggcgtgtg
     4861 caggagtttg acagtggcct gctgcactgg cggattggtg ggggggacac cactgagcat
     4921 atccagaccc acttcgagag caagacagag ctgctgcctt cccggcctca cgcaccctgc
     4981 ccaccagccc ctcggaagca tgtgacaaca gcagagggta caccagggac aacagaccag
     5041 gaggggcccc cacctgatgg acctccagaa aaacggatca cagccactat ggatgacatg
     5101 ttgtctactc ggtctagcac cttgaccgag gatggagcta agagttcaga ggccatcaag
     5161 gagagcagca agtttccatt tggcattagc ccagcacaga gccaccggaa catcaagatc
     5221 ctagaggacg aaccccacag taaggatgag accccactgt gtacccttct ggactggcag
     5281 gattctcttg ccaagcgctg cgtctgtgtg tccaatacca ttcgaagcct gtcatttgtg
     5341 ccaggcaatg actttgagat gtccaaacac ccagggctgc tgctcatcct gggcaagctg
     5401 atcctgctgc accacaagca cccagaacgg aagcaggcac cactaactta tgaaaaggag
     5461 gaggaacagg accaaggggt gagctgcaac aaagtggagt ggtggtggga ctgcttggag
     5521 atgctccggg aaaacacctt ggttacactc gccaacatct cggggcagtt ggacctatct
     5581 ccataccccg agagcatttg cctgcctgtc ctggacggac tcctacactg ggcagtttgc
     5641 ccttcagctg aagcccagga ccccttttcc accctgggcc ccaatgccgt cctttccccg
     5701 cagagactgg tcttggaaac cctcagcaaa ctcagcatcc aggacaacaa tgtggacctg
     5761 attctggcca cacccccctt cagccgcctg gagaagttgt atagcactat ggtgcgcttc
     5821 ctcagtgacc gaaagaaccc ggtgtgccgg gagatggctg tggtactgct ggccaacctg
     5881 gctcaggggg acagcctggc agctcgtgcc attgcagtgc agaagggcag tatcggcaac
     5941 ctcctgggct tcctagagga cagccttgcc gccacacagt tccagcagag ccaggccagc
     6001 ctcctccaca tgcagaaccc accctttgag ccaactagtg tggacatgat gcggcgggct
     6061 gcccgcgcgc tgcttgcctt ggccaaggtg gacgagaacc actcagagtt tactctgtac
     6121 gaatcacggc tgttggacat ctcggtatca ccgttgatga actcattggt ttcacaagtc
     6181 atttgtgatg tactgttttt gattggccag tcatagtaat gaactagtcc gtaacttgaa
     6241 agtatttcga tttcttggct ttatatatct tgtggaaagg acgaggagat agaagctagg
     6301 gcgtttat
//
