LOCUS       sequence-284208-        2624 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2468
FEATURES             Location/Qualifiers
     source          1..2624
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             508..519
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     regulatory      872..881
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggctggacc gggcagcacg ggggggcaga tcggggctgc ggccctggca
       61 ggcggcgcgc ggtccaaggt agccccgagc gtggacttcg accatagctg ctcggacagt
      121 gtcgagtacc tgacgctcaa cttcgggccc ttcgaaacag tgcatcgctg gcggcgcctc
      181 ccgccctgcg acgagttcgt gggtgcccgg cgcagcaagc acacagtggt ggcctataaa
      241 gatgccattt atgtatttgg tggagacaat gggaagacca tgctcaatga cctcctgcgg
      301 ttcgatgtga aagactgctc ctggtgcagg gcctttacca ctgggacccc accggccccc
      361 cgttaccacc actcggccgt cgtctatggg agcagcatgt ttgtctttgg gggttacact
      421 ggggacattt attccaattc taacttgaag aataaaaacg acctctttga atacaagttt
      481 gcaactggcc agtggacgga gtggaaaatt gaaggacggt tgccagtcgc taggtcagcc
      541 catggggcca cggtgtacag tgacaagctg tggatctttg ctggctatga cggcaacgcc
      601 aggttgaatg acatgtggac aattggcctc caggaccgag agctcacctg ctgggaggag
      661 gtggcccaga gtggcgagat ccccccatct tgctgcaact tccccgtggc tgtgtgccgg
      721 gacaagatgt ttgtattctc tgggcaaagc ggagccaaaa taaccaacaa cctcttccag
      781 tttgaattca aggacaagac gtggacacgc atcccaactg aacacctgct ccggggctcc
      841 ccaccacccc cgcagcggcg ctacgggcat accatggtgg cctttgaccg ccacctctat
      901 gtgtttgggg gtgcggccga caacacgctg cccaacgagc tgcactgcta tgacgtggac
      961 ttccagacct gggaggtcgt ccagcccagc tccgacagcg aggttggtgg ggctgaagtg
     1021 cccgagcgag cctgtgcttc cgaggaggtg cccaccctga cctatgagga gcgggttggc
     1081 ttcaagaagt cccgagatgt gtttggcctg gactttggca ccacctcagc caagcagccc
     1141 acccagcctg cctcggagct gcccagtggg aggctcttcc acgcggctgc tgtcatctcg
     1201 gacgccatgt acatcttcgg gggcacggtg gacaacaaca tccgcagcgg ggagatgtac
     1261 aggttccagt tctcctgtta ccctaaatgc acgctgcacg aggactacgg gcggctgtgg
     1321 gagagccgcc agttctgcga cgtggagttc gtgctgggtg agaaggagga gtgcgtgcag
     1381 ggccacgtag ccattgtcac agcgcggagc cgctggcttc gcaggaagat cacgcaggcg
     1441 cgggagaggc tggcccagaa gctggagcag gaggccgccc cagttcccag ggaggccccc
     1501 ggcgtggctg ctggtggggc ccggccgccc ctgctgcacg tggccatccg ggaggccgag
     1561 gcccggccct tcgaggtgct catgcagttc ctctacaccg acaagatcaa atacccacgg
     1621 aaaggccatg tggaggatgt gctgctcatc atggatgtgt acaaactggc actgagcttc
     1681 cagttgtgcc gcctggagca gctgtgccgc cagtacatcg aggcctccgt ggacctgcag
     1741 aacgtgctgg ttgtgtgcga gagtgccgcc cggctgcagc tgagccaact caaggagcac
     1801 tgcctgaact tcgtggtaaa ggagtcccac ttcaaccagg tgatcatgat gaaggagttc
     1861 gagcgcctct cctctccact gatagtggag attgtgcggc ggaagcagca gccgccccct
     1921 cgcactccct tggaccagcc agtggacatt ggcacatctc tgatccagga catgaaggca
     1981 tacctggagg gagcgggcgc ggaattctgt gacatcactc tgttgcttga cgggcaccca
     2041 cggccagccc acaaggctat cctggccgcc cgctccagct actttgaagc catgttccgg
     2101 tccttcatgc ccgaagatgg gcaggtgaac atctccatcg gggagatggt gcccagcagg
     2161 caggccttcg agtccatgct gcgctacatc tactacggcg aggtcaacat gccgcccgag
     2221 gactcgctct acttgtttgc ggccccctac tactacggct tctacaacaa ccggctgcag
     2281 gcgtactgca agcagaacct ggagatgaac gtgacggtgc agaacgtgct gcagatcctg
     2341 gaggcagctg acaaaacgca ggcactggac atgaagcggc actgcctgca catcattgtg
     2401 caccagttca ccaaggtctc caagttgccc accctgcggt cgctgagcca gcagctgctg
     2461 ctggacatca tagactccct ggcctcccac atctcagaca agcagtgcgc agagctgggc
     2521 gccgacatct agtaatgaac tagtccgtaa cttgaaagta tttcgatttc ttggctttat
     2581 atatcttgtg gaaaggacga atattggagc cctagctgcc tcgg
//
