LOCUS       sequence-284214-        7820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7820)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 7820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2476
FEATURES             Location/Qualifiers
     source          1..7820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggccaccct taactcagcc tctaccactg gtaccacccc ctcccctggg
       61 cacaatgccc cgtccctgcc ttcggacacc ttctcctcca gcaccccctc tgatcctgtc
      121 accaaagatc cccctgctgc ctcctccacc tctgagaaca tgaggtcctc agagccaggg
      181 ggacagctcc tggagtcggg ctgtggcctc gtcccaccaa aggagattgg ggagccccag
      241 gaagggcctg actgtggtca cttcccacca aatgacccag gggtggaaaa ggacaaggag
      301 caggaggagg aagaagaagg gctccctccc atggacctaa gcaaccactt attcttcaca
      361 gctggaggtg aggcctacct agtggccaag ctgtccctgc caggtggcag tgaactcctg
      421 ttaccaaagg gcttcccctg gggtgaggcg ggcatcaagg aagagcccag tctgcccttc
      481 cttgcctacc cacccccctc acacctcact gcccttcaca tccaacatgg ctttgaccca
      541 atccaaggct ttagctcttc tgaccaaatt ctgtcccatg atacctcagc accatctccg
      601 gctgcctgtg aggaaaggca tggagctttc tggagctacc agctggctcc aaatccaccc
      661 ggagatccca aagatggccc catggggaac agcgggggca accacgtggc ggtcttctgg
      721 ctctgccttc tgtgccgcct gggtttcagc aagccccagg cctttatgga tcacacacag
      781 tctcatgggg tgaagctaac ccctgcccaa tatcagggcc tgtcaggtag cccagctgta
      841 ctccaggagg gagatgaagg ctgcaaggcc ctcataagct ttctggagcc aaaactccct
      901 gctcgcccct cttctgacat accccttgac aacagcagca cagtgaacat ggaggcgaat
      961 gtggcccaga cagaggatgg cccccctgag gcagaagtcc aggcccttat cctcctggat
     1021 gaagaagtta tggccctcag cccaccctct ccacccacag ccacctggga ccccagccca
     1081 acccaagcca aagaatcgcc agtagcagca ggcgaggcag ggccagattg gttccctgag
     1141 gggcaagaag aggatggagg gctctgcccc ccactcaacc aaagctcacc cacctccaag
     1201 gaggggggca ctctccctgc cccagtgggc tcccccgaag accccagtga cccaccccag
     1261 ccctatcgcc tagctgatga ctacacccca gcccctgcag ccttccaggg cctcagcctg
     1321 tccagccaca tgtccctgct ccactcacgc aactcctgca agacactcaa gtgtcccaag
     1381 tgcaactggc actacaagta ccagcagacc ctggatgtgc acatgcgaga gaagcaccct
     1441 gagagcaaca gtcactgcag ctactgcagt gctgggggcg cccacccccg ccttgctcgt
     1501 ggagagagct acaactgtgg ctacaaaccc taccgctgtg acgtctgcaa ctactctaca
     1561 accaccaaag gcaacctcag catccatatg cagtctgaca agcacctggc caacctacag
     1621 ggcttccagg cgggccctgg tgggcaggga agtccaccag aggcatcact cccaccctcc
     1681 gcgggagaca aagagcctaa gaccaaatca tcctggcagt gcaaggtgtg cagctacgag
     1741 acaaacatct cccgtaacct gcgcatccat atgacctctg agaagcacat gcagaatgtc
     1801 ctaatgctgc accaggggct gccgctgggc ctgccacctg gattgatggg gccaggccct
     1861 cctcccccac caggggctac ccccactagc ccccctgaac tcttccagta ctttgggccc
     1921 caggccctag ggcagcctca gactcccttg gctggcccgg ggctgaggcc agacaagccc
     1981 ctggaagccc agctacttct caatggtttc caccacgtag gagcacctgc ccgcaagttc
     2041 cccacatccg cccctggaag cctatcccct gatgcccacc tgcctccaag tcagctcctg
     2101 ggttcctcat ctgacagcct gcccacctca ccacccccag acgacagcct gtccctgaag
     2161 gtgttccgct gcctagtgtg ccaggccttc agcacagaca gcctggagct gctgctctac
     2221 cactgcagca taggccggag cctcccggaa gctgaatgga aggaggtggc tggtgacacc
     2281 caccgctgca agctttgctg ctatggcacc cagctcaagg ccaacttcca actccacctc
     2341 aagactgaca aacatgctca gaagtaccag ctggcagccc acctgcggga ggggggtgga
     2401 gccatgggca ccccttcccc agcatccctg ggagatgggg ctccttatgg gtctgtctcc
     2461 ccactacacc tgcgctgcaa catctgtgac tttgagtcca acagcaagga gaagatgcag
     2521 ctgcatgcca ggggtgcagc ccacgaagaa aacagccaaa tctataagtt tctgctggac
     2581 atggagggag cggaggcagg ggcagagctg gggctatacc actgcctgtt gtgtgcgtgg
     2641 gagacaccct cccgcttggc tgtgctgcaa cacctgcgca cacctgccca ccgcgatgcc
     2701 caggcccaga ggcgtctgca gctgctacag aatggcccaa ccactgagga aggactcgca
     2761 gctcttcaga gcatcctgag cttcagccac gggcagctcc ggactcccgg gaaggctcct
     2821 gtcaccccct tagctgagcc acccacccct gagaaagatg cccagaacaa gacagaacaa
     2881 ttggcttcag aagagacaga aaacaagact ggcccttcca gagacagtgc caaccagacc
     2941 acggtatact gctgtccata ctgcagcttc ctgagcccag agtccagcca ggtgagggct
     3001 catacactct cccagcatgc agtgcagccc aagtacagat gcccactgtg ccaggaacag
     3061 ctggtgggcc ggcctgccct gcacttccac cttagccacc ttcacaacgt ggtgcccgag
     3121 tgcgttgaga agctgctgct tgtagctaca actgtagaaa tgacatttac aaccaaagtg
     3181 ctgtctgcac ccacattaag ccctctggac aatggccaag aaccccccac tcatgggcca
     3241 gagcctacac cgagcagaga ccaggcagca gaaggcccta acctgacccc agaagccagt
     3301 ccagatcctc ttcctgagcc tcccctggcc tcagttgagg tcccagacaa accctcagga
     3361 agccctggcc aacccccttc tccagcccca tctccagtcc ctgaacctga tgcccaagct
     3421 gaagacgtag ctcctccgcc caccatggct gaggaggaag aggggaccac tggggagctc
     3481 cgctctgcag agccagctcc agctgactct cgccaccctc tgacctatcg gaaaaccacc
     3541 aactttgccc tggacaagtt tctcgaccct gcccggccct ataagtgcac tgtgtgtaag
     3601 gagtccttca cccagaagaa tattctgttg gttcattata attctgtctc ccaccttcac
     3661 aagatgaaga aggctgccat tgacccctct gcccctgcac ggggagaggc cggtgcccca
     3721 cccaccacca ctgctgccac agacaagccc tttaagtgca cagtctgcag agtctcctac
     3781 aaccagagct ccaccctgga gatccacatg cggtcagttc tgcatcagac tcgctctcgg
     3841 ggaaccaaga ctgattccaa gattgaaggg ccagaacgca gccaagaaga gcccaaggaa
     3901 ggcgagacag agggggaggt gggcactgag aagaagggcc ctgacaccag tggcttcata
     3961 tctggattgc ctttcctgtc ccctccccca cctcccttgg acctgcaccg attcccagcc
     4021 cctctcttca ccccaccagt cctgcccccc ttccctctgg tgcccgaatc actgcttaag
     4081 ctccagcagc agcagctgct cctgcccttc tacctccacg atctcaaggt ggggcccaag
     4141 ctgacactag ctgggcctgc acctgtgctg tccctgccag ctgccacccc tcctcctcca
     4201 ccccaacctc ccaaggctga gctggctgag cgggagtggg agcggccccc catggccaaa
     4261 gagggtaatg aggcagggcc ttcctcaccc cccgacccat tgcccaacga ggctgcccgc
     4321 actgcagcca aagcccttct agaaaacttt ggctttgagc tggtgatcca gtacaatgaa
     4381 gggaagcaag ctgtgccccc tccccctacc ccacccccac ctgaggccct cgggggtggg
     4441 gacaagctgg cctgtggggc ctgtgggaaa ctcttctcca atatgcttat cctcaagaca
     4501 cacgaggaac atgtccaccg ccgctttctg ccctttgaag ccctgagccg ttatgctgct
     4561 cagtttcgaa agagctatga cagcctatac ccgccccttg cagagcctcc caaacctcct
     4621 gatgggtctc tggattcacc tgttccccat ctgggcccac ccttcctggt cccagagcct
     4681 gaggcagggg ggacccgtgc ccctgaagag cgaagtcgag cagggggaca ctggcccata
     4741 gaagaggaag aaagctccag agggaatctt cctcccctgg tgcctgccgg ccgccggttc
     4801 tccagaacca agttcacaga gttccagacc caagccctgc agtctttctt tgagactagc
     4861 gcctacccca aagacggaga ggtggagcga ctcgcaagtc tgttgggtct ggctagccgt
     4921 gtggtggtgg tgtggttcca gaatgcccgc cagaaagcac gcaaaaatgc ctgtgagggt
     4981 gggtccatgc caaccggagg aggcactggg ggagcctccg gctgcaggcg ttgccacgcc
     5041 actttctcct gtgtttttga gttggtgcgc cacctcaaga agtgctatga tgaccagacc
     5101 cttgaagagg aggaggaaga ggcagagaga ggggaagagg aggaagaggt ggaagaagaa
     5161 gaagtagagg aggaacaggg ccttgaacct ccagcagggc ctgagggccc attaccagag
     5221 cctccagatg gggaggagct gagccaagca gaggcaacaa aggcaggagg caaagagcct
     5281 gaagagaagg ctactccatc accttcccca gcccatacct gtgaccagtg tgccatttct
     5341 ttctccagcc aggacctcct gaccagtcac cgccgactac atttcctgcc atctctgcag
     5401 cccagtgctc ccccccaact cctagatctg cccttgctgg tgtttgggga gagaaacccc
     5461 ctggtggcag ccacctcacc aatgccaggt ccacctctca aacggaagca tgaggacggc
     5521 agcttgtctc ccacaggcag tgaagcaggg ggaggagggg agggcgagcc ccccagggac
     5581 aagcgcctgc gcaccaccat cttgcctgag cagctagaga tcctgtaccg ttggtacatg
     5641 caggattcca acccaacacg caagatgctc gactgcatct ccgaggaggt ggggctcaaa
     5701 aagcgagtgg tacaggtctg gttccagaat accagggccc gggagaggaa aggccagttt
     5761 cgaagcaccc ctgggggggt gcctagtcca gcagtgaaac cgcctgccac agccacccct
     5821 gcatccttgc ccaagttcaa cctcttatta ggcaaggtag atgatggcac tgggagggaa
     5881 gccccaaaga gggaagcacc tgcttttccc taccccactg ccacgcttgc ttctgggccc
     5941 cagcctttcc taccacctgg gaaagaggcc accaccccaa caccagagcc acctctacct
     6001 ctcctacctc cccctccacc cagtgaggaa gagggcccag aggaaccacc taaagcttct
     6061 ccagagagtg aggcttgcag tctctctgca ggagatctga gtgattcatc tgcttccagc
     6121 ctagctgaac cagaatcccc tggggctgga gggaccagtg ggggacctgg aggtgggact
     6181 ggggttccag atggaatggg gcagcggcgc tacaggaccc agatgagcag cctgcagctg
     6241 aagatcatga aagcctgcta tgaagcttac cgcaccccca ccatgcagga gtgtgaggtg
     6301 ctgggagagg agattgggct gcccaagaga gtcatccagg tctggttcca gaatgctcgt
     6361 gccaaggaaa agaaggccaa actacagggg acagccgctg ggagcactgg gggcagcagt
     6421 gagggcctct tagcagccca gcgcactgac tgcccctatt gtgatgtcaa gtatgatttc
     6481 tatgtctcct gccgaggcca tctcttttcc cgtcagcacc tggccaagct caaggaggcg
     6541 gttcgagccc agctgaagag tgaaagcaag tgctacgact tggccccagc acctgaggct
     6601 cccccagctc tcaaggcccc acctgccacc acacctgcct ccatgcccct cggggctgcc
     6661 ccaaccttgc ctcgcctggc gccggtcctc ttatctggcc cagctctggc tcagcccccg
     6721 ctgggcaact tagctccttt caattcaggc ccggcagcct cctcaggcct cctcggcctc
     6781 gccacttcgg tcctgcctac caccacagtg gtccagactg ctggcccagg ccgcccctta
     6841 cctcagagac ccatgcccga ccaaaccaac acctccacag caggcaccac tgaccctgtc
     6901 ccaggccctc ctactgagcc cttgggggac aaggtctcca gtgagcgaaa gccagttgca
     6961 ggccccacca gctcctccaa tgatgccctc aagaacctca aagcattgaa gaccactgtc
     7021 ccagccctgt tggggggcca gttcctgccc tttccattgc cccctgctgg gggaacagca
     7081 ccgccagctg tctttggccc ccagctacag ggggcctact tccaacagct ctatggcatg
     7141 aagaaggggc tatttcccat gaaccccatg atacctcaga ccctcattgg gctgctcccc
     7201 aatgccctcc tccagccgcc accccagccc cctgagccca cagccacagc acctccaaag
     7261 cctcctgaac tgcctgctcc aggggagggg gaagctggtg aggttgatga gctgctgaca
     7321 ggcagcactg gcatctccac cgtggatgta acccatcgct acctgtgccg ccagtgcaag
     7381 atggcatttg acggggaggc cccggctact gcccaccaga gatccttctg cttctttggg
     7441 cggggctctg ggggctccat gccaccccca ttgcgggtgc ccatctgcac ctaccactgc
     7501 ctggcatgtg aggtgctgct gagtgggcgt gaagccctag cctcccacct gcgctcctcg
     7561 gcccacaggc gcaaggcagc cccacctcaa gggggcccac ccatctccat caccaacgcc
     7621 gccactgctg cctcggctgc tgtggctttt gccaaagagg aagcaagatt acctcacacg
     7681 gactccaacc caaaaactac gactacctct acacttctag ctttatagta atgaactagt
     7741 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgagggt
     7801 gcctgcttcc ttgcaaccta
//
