LOCUS       sequence-284220-        2963 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2510
FEATURES             Location/Qualifiers
     source          1..2963
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      433..442
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgcgacagct gcagaagaaa ataaagaatg gaacactgaa cataaagcag
       61 gacgacccct ttgaactctt catagcagcc acaaacattc gctactgcta ctacaacgag
      121 acccacaaga tcctgggcaa taccttcggc atgtgtgtgc tgcaggattt tgaagcctta
      181 actccaaact tgctggccag gactgtagaa acagtggaag gtggtgggct agtggtcatc
      241 ctcctacgga ccatgaactc actcaagcaa ttgtacacag tgactatgga tgtgcattcc
      301 aggtacagaa ctgaggccca tcaggatgtg gtgggaagat ttaatgaaag gtttattctg
      361 tctctggcct cttgtaagaa gtgtctcgtc attgatgacc agctcaacat cctgcccatc
      421 tcctcccacg ttgccaccat ggaggccctg cctccccaga ctccggatga gagtcttggt
      481 ccttctgatc tggagctgag ggagttgaag gagagcttgc aggacaccca gcctgtgggt
      541 gtgttggtgg actgctgtaa gactctagac caggccaaag ctgtcttgaa atttatcgag
      601 ggcatctctg aaaagaccct gaggagtact gttgcactca cagctgctcg aggacgggga
      661 aaatctgcag ccctgggatt ggcgattgct ggggcggtgg catttgggta ctccaatatc
      721 tttgttacct ccccaagccc tgataacctc catactctgt ttgaatttgt atttaaagga
      781 tttgatgctc tgcaatatca ggaacatctg gattatgaga ttatccagtc tctaaatcct
      841 gaatttaaca aagcagtgat cagagtgaat gtatttcgag aacacaggca gactattcag
      901 tatatacatc ctgcagatgc tgtgaagctg ggccaggctg aactagttgt gattgatgaa
      961 gctgccgcca tccccctccc cttggtgaag agcctacttg gcccctacct tgttttcatg
     1021 gcatccacca tcaatggcta tgagggcact ggccggtcac tgtccctcaa gctaattcag
     1081 cagctccgtc aacagagcgc ccagagccag gtcagcacca ctgctgagaa taagaccacg
     1141 acgacagcca gattggcatc agcgcggaca ctgtatgagg tttccctcca ggagtcaatc
     1201 cgatacgccc ctggggatgc agtggagaag tggctgaatg acttgctgtg cctggattgc
     1261 ctcaacatca ctcggatagt ctcaggctgc cccttgcctg aagcttgtga actgtactat
     1321 gttaatagag ataccctctt ttgctaccac aaggcctctg aagttttcct ccaacggctt
     1381 atggccctct acgtggcttc tcactacaag aactctccca atgatctcca gatgctctcc
     1441 gatgcacctg ctcaccatct cttctgcctt ctgcctcccg tgccccccac ccagaatgcc
     1501 cttccagaag tgcttgctgt tatccaggtg tgccttgaag gggagatttc tcgccagtcc
     1561 atcttgaaca gtctgtctcg aggcaagaag gcttcagggg acctgattcc atggacagtg
     1621 tcagaacagt tccaagatcc agactttggt ggtctgtctg gtggaagggt cgttcgcatt
     1681 gctgttcacc cagattatca agggatgggc tatggcagcc gtgctctgca gctgctgcag
     1741 atgtactatg aaggcaggtt tccttgtctg gaggaaaagg tccttgagac accacaggaa
     1801 attcacaccg taagcagcga ggctgtcagc ttgttggaag aggtcatcac tccccggaag
     1861 gacctgcctc ctttactcct caaattgaat gagaggcctg ccgaacgcct ggattacctg
     1921 ggtgtttcct atggcttgac ccccaggctc ctcaagttct ggaaacgagc tggatttgtt
     1981 cctgtttatc tgagacagac cccgaatgac ctgaccggag agcactcgtg catcatgctg
     2041 aagacgctca ctgatgagga tgaggctgac cagggaggct ggcttgcagc cttctggaaa
     2101 gatttccgac ggcggttcct agccttgctc tcctaccagt tcagtacctt ctctccttcc
     2161 ctggctctga acatcattca gaacaggaac atggggaagc cagcccagcc tgccctgagc
     2221 cgggaggagc tggaagcact cttcctcccc tatgacctga agcggctgga gatgtattca
     2281 cggaatatgg tggactatca cctcatcatg gacatgatcc cggccatctc tcgcatctat
     2341 ttcctgaacc agctggggga cctggccctg tctgcggctc agtcggctct tctcttgggg
     2401 attggcctgc agcataagtc tgtggaccag ctggaaaagg agattgagct gccctcgggc
     2461 cagttgatgg gacttttcaa ccggatcatc cgcaaagttg tgaagctatt taatgaagtt
     2521 caggaaaagg ccattgagga gcagatggtg gcagcgaagg atgtggtcat ggagcccacg
     2581 atgaagaccc tcagtgacga cctagatgaa gcagcaaagg aatttcagga gaaacacaag
     2641 aaggaagtag ggaagctgaa gagcatggac ctctctgaat acataatccg tggggacgat
     2701 gaagagtgga atgaagtttt gaacaaagct gggccgaacg cctcgatcat cagcctgaaa
     2761 agtgacaaga aaaggaagtt agaggccaaa caagaaccca aacagagcaa gaagttgaag
     2821 aacagagaga caaagaacaa aaaagatatg aaactgaagc ggaagaaata gtaatgaact
     2881 agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgac
     2941 cctgtaatag aattgctgaa act
//
