LOCUS       sequence-284247-        2288 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2549
FEATURES             Location/Qualifiers
     source          1..2288
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaggcggc ggcaggcggc cgcggctgtt tccagccgca cccggggctg
       61 cagaagacgc tggagcagtt ccacctgagc tccatgagct cgctgggcgg cccggccgct
      121 ttctcggcgc gctgggcgca ggaggcctac aagaaggaga gcgccaagga ggcgggcgcg
      181 gccgcggtgc cggcgccggt gcccgcagcc accgagccgc cgcccgtgct gcacctgccc
      241 gccatccagc cgccgccgcc cgtgctgccc gggcccttct tcatgccgtc cgaccgctcc
      301 accgagcgct gcgagaccgt actggaaggc gagaccatct cgtgcttcgt ggtgggaggc
      361 gagaagcgcc tgtgtctgcc gcagattctc aactcggtgc tgcgcgactt ctcgctgcag
      421 cagatcaacg cggtgtgcga cgagctccac atctactgct cgcgctgcac ggccgaccag
      481 ctggagatcc tcaaagtcat gggcatcctg cccttctcgg cgccctcgtg cgggctcatc
      541 accaagacgg acgccgagcg cctgtgcaac gcgctgctct acggcggcgc ctacccgccg
      601 ccctgcaaga aggagctggc cgccagcctg gcgctgggcc tggagctcag cgagcgcagc
      661 gtccgcgtgt accacgagtg cttcggcaag tgtaaggggc tgctggtgcc cgagctctac
      721 agcagcccga gcgccgcctg catccagtgc ctggactgcc gcctcatgta cccgccgcac
      781 aagttcgtgg tgcactcgca caaggccctg gagaaccgga cctgccactg gggcttcgac
      841 tcggccaact ggcgggccta catcctgctg agccaggatt acacgggcaa ggaggagcag
      901 gcgcgcctcg gccgctgcct ggacgacgtg aaggagaaat tcgactatgg caacaagtac
      961 aagcggcggg tgccccgggt ctcctctgag cctccggcct ccataagacc caaaacagat
     1021 gacacctctt cccagtcccc cgcgccttcc gaaaaggaca agccgtccag ctggctgcgg
     1081 accttggccg gctcttccaa taagagcctg ggctgtgttc accctcgcca gcgcctctct
     1141 gctttccgac cctggtcccc cgcagtgtca gcgagtgaga aagagctctc cccacacctc
     1201 ccggccctca tccgagacag cttctactcc tacaagagct ttgagacagc cgtggcgccc
     1261 aacgtggccc tcgcaccgcc ggcccagcag aaggttgtga gcagccctcc gtgtgccgcc
     1321 gccgtctccc gggcccccga gcctctcgcc acttgcaccc agcctcggaa gcggaagctg
     1381 actgtggaca ccccaggagc cccagagacg ctggcgcccg tggctgcccc agaggaggac
     1441 aaggactcgg aggcggaggt ggaagttgaa agcagggagg aattcacctc ctccttgtcc
     1501 tcgctctctt ccccgtcctt tacctcatcc agctccgcca aggacctggg ctccccgggt
     1561 gcgcgtgccc tgccctcggc cgtccctgat gctgcggccc ctgccgacgc ccccagtggg
     1621 ctggaggcgg agctggagca cctgcggcag gcactggagg gcggcctgga caccaaggaa
     1681 gccaaagaga agttcctgca tgaggtggtc aagatgcgcg tgaagcagga ggagaagctc
     1741 agcgcagccc tgcaggccaa gcgcagcctc caccaggagc tggagttcct acgcgtggcc
     1801 aagaaggaga agctgcggga ggccacggag gccaagcgta acctgcggaa ggagatcgag
     1861 cgtctccgcg ccgagaacga gaagaagatg aaagaggcca acgagtcacg gctgcgcctg
     1921 aagcgggagc tggagcaggc gcggcaggcc cgggtgtgcg acaagggctg cgaggcgggc
     1981 cgcctgcgcg ccaagtactc ggcccagatc gaagacctgc aggtgaagct gcagcacgcg
     2041 gaggcggacc gggagcagct gcgggccgac ctgctgcggg agcgcgaggc ccgggagcac
     2101 ctggagaagg tggtgaagga gctgcaggaa cagctgtggc cgcgggcccg ccccgaggct
     2161 gcgggcagcg agggcgctgc ggagctggag ccgtagtaat gaactagtcc gtaacttgaa
     2221 agtatttcga tttcttggct ttatatatct tgtggaaagg acgacaccta tacccacggg
     2281 ttacaatc
//
