LOCUS       sequence-284265-        1280 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1280)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2587
FEATURES             Location/Qualifiers
     source          1..1280
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcgacagc agcgcaggga cccctaagct tgctgtgggg ctggctgtgg
       61 agcgagcgct tctggctacc cgagaacgtg agctgggctg atctggaggg gccggccgac
      121 ggctacggtt acccccgcgg ccggcacatc ctctcggtgt tcccgctggc ggcgggcatc
      181 ttcttcgtga ggctgctctt cgagcgattt attgccaaac cctgtgcact ctgtattggc
      241 atcgaggaca gtggtcctta tcaggcccaa cccaatgcca tccttgaaaa ggtgttcata
      301 tctattacca agtatcctga taagaaaagg ctggagggcc tgtcaaagca gctggattgg
      361 aatgtccgaa aaatccaatg ctggtttcgc catcggagga atcaggacaa gcccccaacg
      421 cttactaaat tctgtgaaag catgtggaga ttcacatttt atttatgtat attctgctat
      481 ggaattagat ttctctggtc gtcaccttgg ttctgggaca tccgacagtg ctggcataac
      541 tatccatttc agcctctttc aagtgggctt tatcactatt atatcatgga attggccttc
      601 tattggtccc ttatgttttc tcagtttaca gacattaaaa gaaaggactt cctgatcatg
      661 tttgtgcatc acttggtcac cattgggctt atctccttct cctacatcaa caatatggtt
      721 cgagtgggaa ctctgatcat gtgtctacat gatgtctcag acttcttgct ggaggcagcc
      781 aaactggcca attatgccaa gtatcagcgg ctctgtgaca ccctttttgt gatcttcagt
      841 gctgttttta tggttacacg actaggaatc tatccattct ggattctgaa cacgaccctc
      901 tttgagagtt gggagataat cgggccttat gcttcatggt ggctcctcaa tggcctgctg
      961 ctgaccctac agcttctgca tgtcatctgg tcctacctaa ttgcacggat tgctttgaaa
     1021 gccttgatca ggggaaaggt atcgaaggat gatcgcagtg atgtggagag cagctcagag
     1081 gaagaagatg tgaccacctg cacaaaaagt ccctgtgaca gtagctccag caatggtgcc
     1141 aatcgggtga atggtcacat gggaggcagc tactgggctg aagagtagta atgaactagt
     1201 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgatcat
     1261 tcgcattacg gccaaccggt
//
