LOCUS       sequence-284268-        1550 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2594
FEATURES             Location/Qualifiers
     source          1..1550
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggacgaagg aattcctcat ttgcaagaga gacagttact ggaacataga
       61 gattttatag gactggacta ttcctctttg tatatgtgta aacccaaaag gagcatgaaa
      121 cgagacgaca ccaaggatac ctacaaatta ccgcacagat taatagaaaa gaaaagaaga
      181 gaccgaatta atgaatgcat tgctcagctg aaagatttac tgcctgaaca tctgaaattg
      241 acaactctgg gacatctgga gaaagctgta gtcttggaat taactttgaa acacttaaaa
      301 gctttaaccg ccttaaccga gcaacagcat cagaagataa ttgctttaca gaatggggag
      361 cgatctctga aatcgcccat tcagtccgac ttggatgcgt tccactcggg atttcaaaca
      421 tgcgccaaag aagtcttgca atacctctcc cggtttgaga gctggacacc cagggagccg
      481 cggtgtgtcc agctgatcaa ccacttgcac gccgtggcca cccagttctt gcccaccccg
      541 cagctgttga ctcaacaggt ccctctgagc aaaggcaccg gcgctccctc ggccgccggg
      601 tccgcggccg ccccctgcct ggagcgcgcg gggcagaagc tggagcccct cgcctactgc
      661 gtgcccgtca tccagcggac tcagcccagc gccgagctcg ccgccgagaa cgacacggac
      721 accgacagcg gctacggcgg cgaagccgag gcccggccgg accgcgagaa aggcaaaggc
      781 gcgggggcga gccgcgtcac catcaagcag gagcctcccg gggaggactc gccggcgccc
      841 aagaggatga agctggattc ccgcggcggc ggcagcggcg gcggcccggg gggcggcgcg
      901 gcggcggcgg cagccgcgct tctggggccc gaccctgccg ccgcggccgc gctgctgaga
      961 cccgacgccg ccctgctcag ctcgctggtg gcgttcggcg gaggcggagg cgcgcccttc
     1021 ccgcagcccg cggccgccgc ggcccccttc tgcctgccct tctgcttcct ctcgccttct
     1081 gcagctgccg cctacgtgca gcccttcctg gacaagagcg gcctggagaa gtatctgtac
     1141 ccggcggcgg ctgccgcccc gttcccgctg ctataccccg gcatccccgc cccggcggca
     1201 gccgcggcag ccgccgccgc cgctgccgcc gccgccgccg cgttcccctg cctgtcctcg
     1261 gtgttgtcgc cccctcccga gaaggcgggc gccgccgccg cgaccctcct gccgcacgag
     1321 gtggcgcccc ttggggcgcc gcacccccag cacccgcacg gccgcaccca cctgcccttc
     1381 gccgggcccc gcgagccggg gaacccggag agctctgctc aggaagatcc ctcgcagcca
     1441 ggaaaggaag ctccctagta atgaactagt ccgtaacttg aaagtatttc gatttcttgg
     1501 ctttatatat cttgtggaaa ggacgataca gcccagccaa ttcagtggga
//
