LOCUS       sequence-284283-        2054 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2623
FEATURES             Location/Qualifiers
     source          1..2054
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcgacttc gaacaatccg cggaaattca gcgagaagat cgcgctgcac
       61 aatcagaagc aggcggagga gacggcggcc ttcgaggagg tcatgaagga cctgagcctg
      121 acgcgggccg cgcggctcca gctccagaaa tcccagtacc tgcaactggg ccccagccga
      181 ggccagtact atggcgggtc cctgcccaac gtgaaccaga tcgggagtgg caccatggac
      241 ctgcccttcc agcccagcgg atttctgggg gaggccctgg cagcggctcc tgtctctctg
      301 acccccttcc aatcctcggg cctggacacc agccggacca cccggcacca tgggctggtg
      361 gacagggtgt accgggagcg tggccggctc ggctccccac accgccggcc cctgtcagtg
      421 gacaaacacg gacggcaggc cgacagctgc ccctatggca ccatgtacct ctcaccaccc
      481 gcggacacca gctggagaag gaccaattct gactccgccc tgcaccagag cacaatgacg
      541 cccacgcagc cagaatcctt tagcagtggg tcccaggacg tgcaccagaa aagagtctta
      601 ctgttaacag tcccaggaat ggaagagacc acatcagagg cagacaaaaa cctttccaag
      661 caagcatggg acaccaagaa gacggggtcc aggcccaagt cctgtgaggt ccccggaatc
      721 aacatcttcc cgtctgccga ccaggaaaac actacagccc tgatccccgc cacccacaac
      781 acaggggggt ccctgcccga cctgaccaac atccacttcc cctccccgct cccgaccccg
      841 ctggaccccg aggagcccac cttccctgca ctgagcagct ccagcagcac cggcaacctc
      901 gcggccaacc tgacgcacct gggcatcggt ggcgccggcc agggaatgag cacacctggc
      961 tcctctccac agcaccgccc agctggcgtc agccccctgt ccctgagcac agaggcaagg
     1021 cgtcagcagg catcgcccac cctgtccccg ctgtcaccca tcactcaggc tgtagccatg
     1081 gacgccctgt ctctggagca gcagctgccc tacgccttct tcacccaggc gggctcccag
     1141 cagccaccgc cgcagcccca gcccccgccg cctcctccac ccgcgtccca gcagccacca
     1201 cccccgccac ccccacaggc gcccgtccgc ctgccccctg gtggccccct gttgcccagc
     1261 gccagcctga ctcgtgggcc acagccgccc ccgcttgcag tcacggtacc gtcctctctc
     1321 ccccagtccc ccccagagaa ccctggccag ccatcgatgg ggatcgacat cgcctcggcg
     1381 ccggctctgc agcagtaccg cactagcgcc ggctccccgg ccaaccagtc tcccacctcg
     1441 ccagtctcca atcaaggctt ctccccaggg agctccccgc aacacacttc caccctgggc
     1501 agcgtgtttg gggacgcgta ctatgagcag cagatggcgg ccaggcaggc caatgctctg
     1561 tcccaccagc tggagcagtt caacatgatg gagaacgcca tcagctccag cagcctgtac
     1621 agcccgggct ccacactcaa ctactcgcag gcggccatga tgggcctcac gggcagccac
     1681 gggagcctgc cggactcgca gcaactggga tacgccagcc acagtggcat ccccaacatc
     1741 atcctcacag tgacaggaga gtcccccccc agcctctcta aagaactgac cagctctctg
     1801 gccggggtcg gcgacgtcag cttcgactcc gacagccagt ttcccctgga cgaactcaag
     1861 atcgaccccc tgaccctcga cggactgcac atgctcaacg accccgacat ggttctggcc
     1921 gacccagcca ccgaggacac cttccggatg gaccgcctgt agtaatgaac tagtccgtaa
     1981 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga tactttaccg
     2041 taacgtgcac caac
//
