LOCUS       sequence-284301-        1970 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2653
FEATURES             Location/Qualifiers
     source          1..1970
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      8..17
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcggcgga agaggaggct gcggcgggag gtaaagtgtt gagagaggag
       61 aaccagtgca ttgctcctgt ggtttccagc cgcgtgagtc cagggacaag accaacagct
      121 atggggtctt tcagctcaca catgacagag tttccacgaa aacgcaaagg aagtgattca
      181 gacccatccc agtcaggaat catgacagaa aaagtggtgg aaaagctttc tcagaatccc
      241 cttacctatc ttctttcaac aaggatagaa atatcagcct ccagtggcag cagagaagct
      301 catagccaaa ctgaaaagcg gaggagagat aaaatgaata acctgattga agaactgtct
      361 gcaatgatcc ctcagtgcaa ccccatggcg cgtaaactgg acaaacttac agttttaaga
      421 atggctgttc aacacttgag atctttaaaa ggcttgacaa attcttatgt gggaagtaat
      481 tatagaccat catttcttca ggataatgag ctcagacatt taatccttaa gactgcagaa
      541 ggcttcttat ttgtggttgg atgtgaaaga ggaaaaattc tcttcgtttc taagtcagtc
      601 tccaaaatac ttaattatga tcaggctagt ttgactggac aaagcttatt tgacttctta
      661 catccaaaag atgttgccaa agtaaaggaa caactttctt cttttgatat ttcaccaaga
      721 gaaaagctaa tagatgccaa aactggtttg caagttcaca gtaatctcca cgctggaagg
      781 acacgtgtgt attctggctc aagacgatct tttttctgtc ggataaagag ttgtaaaatc
      841 tctgtcaaag aagagcatgg atgcttaccc aactcaaaga agaaagagca cagaaaattc
      901 tatactatcc attgcactgg ttacttgaga agctggcctc caaatattgt tggaatggaa
      961 gaagaaagga acagtaagaa agacaacagt aattttacct gccttgtggc cattggaaga
     1021 ttacagccat atattgttcc acagaacagt ggagagatta atgtgaaacc aactgaattt
     1081 ataacccggt ttgcagtgaa tggaaaattt gtctatgtag atcaaagggc aacagcgatt
     1141 ttaggatatc tgcctcagga acttttggga acttcttgtt atgaatattt tcatcaagat
     1201 gaccacaata atttgactga caagcacaaa gcagttctac agagtaagga gaaaatactt
     1261 acagattcct acaaattcag agcaaaagat ggctcttttg taactttaaa aagccaatgg
     1321 tttagtttca caaatccttg gacaaaagaa ctggaatata ttgtatctgt caacacttta
     1381 gttttgggac atagtgagcc tggagaagca tcatttttac cttgtagctc tcaatcatca
     1441 gaagaatcct ctagacagtc ctgtatgagt gtacctggaa tgtctactgg aacagtactt
     1501 ggtgctggta gtattggaac agatattgca aatgaaattc tggatttaca gaggttacag
     1561 tcttcttcat accttgatga ttcgagtcca acaggtttaa tgaaagatac tcatactgta
     1621 aactgcagga gtatgtcaaa taaggagttg tttccaccaa gtccttctga aatgggggag
     1681 ctagaggcta ccaggcaaaa ccagagtact gttgctgtcc acagccatga gccactcctc
     1741 agtgatggtg cacagttgga tttcgatgcc ctatgtgaca atgatgacac agccatggct
     1801 gcatttatga attacttaga agcagagggg ggcctgggag accctgggga cttcagtgac
     1861 atccagtgga ccctctagta atgaactagt ccgtaacttg aaagtatttc gatttcttgg
     1921 ctttatatat cttgtggaaa ggacgaatag acgcgatggt aactatattt
//
