LOCUS       sequence-284304-        1724 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1724)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2654
FEATURES             Location/Qualifiers
     source          1..1724
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      8..17
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcggcgga agaggaggct gcggcgggag gtgaggttgc cggtggcgag
       61 gcgacggccc caggtaaagt gttgagagag gagaaccagt gcattgctcc tgtggtttcc
      121 agccgcgtga gtccagggac aagaccaaca gctatggggt ctttcagctc acacatgaca
      181 gagtttccac gaaaacgcaa aggaagtgat tcagacccat cccaagaagc tcatagccaa
      241 actgaaaagc ggaggagaga taaaatgaat aacctgattg aagaactgtc tgcaatgatc
      301 cctcagtgca accccatggc gcgtaaactg gacaaactta cagttttaag aatggctgtt
      361 caacacttga gatctttaaa aggcttgaca aattcttatg tgggaagtaa ttatagacca
      421 tcatttcttc aggataatga gctcagacat ttaatcctta agactgcaga aggcttctta
      481 tttgtggttg gatgtgaaag aggaaaaatt ctcttcgttt ctaagtcagt ctccaaaata
      541 cttaattatg atcaggctag tttgactgga caaagcttat ttgacttctt acatccaaaa
      601 gatgttgcca aagtaaagga acaactttct tcttttgata tttcaccaag agaaaagcta
      661 atagatgcca aaactggttt gcaagttcac agtaatctcc acgctggaag gacacgtgtg
      721 tattctggct caagacgatc ttttttctgt cggataaaga gttgtaaaat ctctgtcaaa
      781 gaagagcatg gatgcttacc caactcaaag aagaaagagc acagaaaatt ctatactatc
      841 cattgcactg gttacttgag aagctggcct ccaaatattg ttggaatgga agaagaaagg
      901 aacagtaaga aagacaacag taattttacc tgccttgtgg ccattggaag attacagcca
      961 tatattgttc cacagaacag tggagagatt aatgtgaaac caactgaatt tataacccgg
     1021 tttgcagtga atggaaaatt tgtctatgta gatcaaaggg caacagcgat tttaggatat
     1081 ctgcctcagg aacttttggg aacttcttgt tatgaatatt ttcatcaaga tgaccacaat
     1141 aatttgactg acaagcacaa agcagttcta cagagtaagg agaaaatact tacagattcc
     1201 tacaaattca gagcaaaaga tggctctttt gtaactttaa aaagccaatg gtttagtttc
     1261 acaaatcctt ggacaaaaga actggaatat attgtatctg tcaacacttt agttttggga
     1321 catagtgagc ctggagaagc atcattttta ccttgtagct ctcaatcatc agaagaatcc
     1381 tctagacagt cctgtatgag tgtacctgga atgtctactg gaacagtact tggtgctggt
     1441 agtattggaa cagatattgc aaatgaaatt ctggatttac agaggttaca gtcttcttca
     1501 taccttgatg attcgagtcc aacaggttta atgaaagata ctcatactgt aaactgcagg
     1561 agtgtgatgg tgcacagttg gatttcgatg ccctatgtga caatgatgac acagccatgg
     1621 ctgcatttat agtaatgaac tagtccgtaa cttgaaagta tttcgatttc ttggctttat
     1681 atatcttgtg gaaaggacga tttgtatccg agactttcaa ttgt
//
