LOCUS       sequence-284310-        2645 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2658
FEATURES             Location/Qualifiers
     source          1..2645
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcgcagtg ggaaatgctg cagaatcttg acagcccctt tcaggatcag
       61 ctgcaccagc tttactcgca cagcctcctg cctgtggaca ttcgacagta cttggctgtc
      121 tggattgaag accagaactg gcaggaagct gcacttggga gtgatgattc caaggctacc
      181 atgctattct tccacttctt ggatcagctg aactatgagt gtggccgttg cagccaggac
      241 ccagagtcct tgttgctgca gcacaatttg cggaaattct gccgggacat tcaggatcct
      301 acccagttgg ctgagatgat ctttaacctc cttctggaag aaaaaagaat tttgatccag
      361 gctcagaggg cccaattgga acaaggagag ccagttctcg aaacacctgt ggagagccag
      421 caacatgaga ttgaatcccg gatcctggat ttaagggcta tgatggagaa gctggtaaaa
      481 tccatcagcc aactgaaaga ccagcaggat gtcttctgct tccgatataa gatccaggcc
      541 aaagggaaga caccctctct ggacccccat cagaccaaag agcagaagat tctgcaggaa
      601 actctcaatg aactggacaa aaggagaaag gaggtgctgg atgcctccaa agcactgcta
      661 ggccgattaa ctaccctaat cgagctactg ctgccaaagt tggaggagtg gaaggcccag
      721 cagcaaaaag cctgcatcag agctcccatt gaccacgggt tggaacagct ggagacatgg
      781 ttcacagctg gagcaaagct gttgtttcac ctgaggcagc tgctgaagga gctgaaggga
      841 ctgagttgcc tggttagcta tcaggatgac cctctgacca aaggggtgga cctacgcaac
      901 gcccaggtca cagagttgct acagcgtctg ctccacagag cctttgtggt agaaacccag
      961 ccctgcatgc cccaaactcc ccatcgaccc ctcatcctca agactggcag caagttcacc
     1021 gtccgaacaa ggctgctggt gagactccag gaaggcaatg agtcactgac tgtggaagtc
     1081 tccattgaca ggaatcctcc tcaattacaa ggcttccgga agttcaacat tctgacttca
     1141 aaccagaaaa ctttgacccc cgagaagggg cagagtcagg gtttgatttg ggactttggt
     1201 tacctgactc tggtggagca acgttcaggt ggttcaggaa agggcagcaa taaggggcca
     1261 ctaggtgtga cagaggaact gcacatcatc agcttcacgg tcaaatatac ctaccagggt
     1321 ctgaagcagg agctgaaaac ggacaccctc cctgtggtga ttatttccaa catgaaccag
     1381 ctctcaattg cctgggcttc agttctctgg ttcaatttgc tcagcccaaa ccttcagaac
     1441 cagcagttct tctccaaccc ccccaaggcc ccctggagct tgctgggccc tgctctcagt
     1501 tggcagttct cctcctatgt tggccgaggc ctcaactcag accagctgag catgctgaga
     1561 aacaagctgt tcgggcagaa ctgtaggact gaggatccat tattgtcctg ggctgacttc
     1621 actaagcgag agagccctcc tggcaagtta ccattctgga catggctgga caaaattctg
     1681 gagttggtac atgaccacct gaaggatctc tggaatgatg gacgcatcat gggctttgtg
     1741 agtcggagcc aggagcgccg gctgctgaag aagaccatgt ctggcacctt tctactgcgc
     1801 ttcagtgaat cgtcagaagg gggcattacc tgctcctggg tggagcacca ggatgatgac
     1861 aaggtgctca tctactctgt gcaaccgtac acgaaggagg tgctgcagtc actcccgctg
     1921 actgaaatca tccgccatta ccagttgctc actgaggaga atatacctga aaacccactg
     1981 cgcttcctct atccccgaat cccccgggat gaagcttttg ggtgctacta ccaggagaaa
     2041 gttaatctcc aggaacggag gaaatacctg aaacacaggc tcattgtggt ctctaataga
     2101 caggtggatg aactgcaaca accgctggag cttaagccag agccagagct ggagtcatta
     2161 gagctggaac tagggctggt gccagagcca gagctcagcc tggacttaga gccactgctg
     2221 aaggcagggc tggatctggg gccagagcta gagtctgtgc tggagtccac tctggagcct
     2281 gtgatagagc ccacactatg catggtatca caaacagtgc cagagccaga ccaaggacct
     2341 gtatcacagc cagtgccaga gccagatttg ccctgtgatc tgagacattt gaacactgag
     2401 ccaatggaaa tcttcagaaa ctgtgtaaag attgaagaaa tcatgccgaa tggtgaccca
     2461 ctgttggctg gccagaacac cgtggatgag gtttacgtct cccgccccag ccacttctac
     2521 actgatggac ccttgatgcc ttctgacttc tagtaatgaa ctagtccgta acttgaaagt
     2581 atttcgattt cttggcttta tatatcttgt ggaaaggacg acatcacgcg gaagtgtgac
     2641 gcgtg
//
