LOCUS       sequence-284316-        1610 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2675
FEATURES             Location/Qualifiers
     source          1..1610
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaaactct tcaggaatcc ttaaacacat tacttaaaca gctagaagaa
       61 gaaaagaaga ctcttgaaag tcaagtgaaa tactatgcac ttaaactgga acaagaatca
      121 aaggcttacc agaagatcaa caatgaacgc cgtacatacc tagctgaaat gtctcagggt
      181 tctggtttac atcaagtttc taaaaggcaa caggtggatc aactgcctag gatgcaagag
      241 aatctagtga aaaccctcct cttaaaagaa gaattggacc ctttaaaggc aaaaatagac
      301 atcttattag ttggagatgt cactgtgggc tacctggctg atactgtaca gaaactattt
      361 gcaaacatag cagaagtcac catcaccatc agtgacacga aggaggcagc agcgcttttg
      421 gatgattgca tattcaacat ggttctcttg aaggtgcctt cttcactaag tgccgaggag
      481 ctggaagcca tcaagttaat tagatttggc aaaaagaaaa atacacattc actgtttgtt
      541 tttataatcc ctgaaaattt taaaggttgt atttcagggc atggaatgga tattgcttta
      601 actgaaccac tgacaatgga aaaaatgagt aatgtggtaa aatactggac aacatgtccc
      661 tcaaacactg ttaagactga aaacgcaact gggcctgaag aacttggatt gcccctgcag
      721 aggtcctaca gcgaacacct gggatatttt cctactgatc tatttgcctg ctctgaatct
      781 ttaaggaatg gcaatgggct tgaattaaat gcttcgttgt cagagttcga gaaaaacaaa
      841 aagatctctc ttcttcattc aagcaaggaa aaactaagaa gggaaagaat caaatattgc
      901 tgtgagcagc tgcgtactct cttgccgtat gtaaaaggga gaaagaatga tgcggcttca
      961 gttcttgagg caacagttga ttatgtgaaa tatatccggg agaaaatctc tccagccgtt
     1021 atggcccaga ttacagaagc acttcagagc aacatgaggt tttgtaagaa acaacaaaca
     1081 cccattgagc tgtctctccc aggcactgtc atggcacagc gggaaaacag tgtgatgagc
     1141 acttactccc ctgagagagg gctccaattc ctgactaata cgtgctggaa tgggtgctcc
     1201 actcctgatg cagagagctc cttggatgaa gctgtgagag ttccatcaag ctccgcctca
     1261 gagaatgcta ttggtgatcc atataaaact cacatttcca gtgcagcgct gtctctgaat
     1321 tccttgcata ctgtcagata ttattctaaa gtcacccctt cctacgatgc aactgctgta
     1381 acaaatcaga acatttcaat tcatttacct tcagccatgc ccccggtctc aaagcttctc
     1441 cctcggcact gcacttctgg gttgggccag acgtgcacta cacatcccaa ctgtctgcaa
     1501 cagttttggg cgtattagta atgaactagt ccgtaacttg aaagtatttc gatttcttgg
     1561 ctttatatat cttgtggaaa ggacgacaaa cggcgcagat tcaatctatc
//
