LOCUS       sequence-284319-        4490 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4490)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 4490)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2678
FEATURES             Location/Qualifiers
     source          1..4490
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 accgccacca tgtaccaacc agaagacgac aacaacagtg acgtgaccag cgacgacgac
       61 atgacccgga acagaagaga gtcctcacca cctcactcag tccattcttt cagtgaccgg
      121 gactgggacc ggaggggcag aagcagagac atggagccac gagaccgctg gtcccacacc
      181 aggaacccaa gaagcaggat gcctccgcgg gatctttccc ttcctgtggt ggcgaaaaca
      241 agctttgaaa tggacagaga ggacgacagg gactccaggg cttatgagtc ccgatctcag
      301 gatgctgaat cataccaaaa tgtggtggac ctcgctgagg acaggaaacc tcacaacaca
      361 atccaggaca acatggaaaa ctacaggaag ctgctctccc tcgtgcagct tgctgaagac
      421 gatggccact cccacatgac gcagggccac tcatcaagat ccaagagaag tgcctaccca
      481 agcaccagtc gaggtctaaa aactatgcct gaagccaaaa aatcaaccca ccggcggggg
      541 atttgtgaag atgaatcttc ccacggagtg ataatggaaa aattcatcaa ggatgtgtca
      601 cgcagttcca aatcgggaag agcaagggag tcaagcgacc ggtcacagag attccccaga
      661 atgtcagatg ataactggaa ggacatttca ttgaacaaga gggagtcagt gatccagcag
      721 cgggtttatg aagggaatgc atttagggga ggctttaggt ttaattcaac ccttgtttcc
      781 agaaagagag ttcttgaaag aaagaggcgc tatcattttg acacagatgg gaagggctcg
      841 attcacgatc aaaaaggctg tcccaggaag aagccctttg aatgtggtag tgagatgaga
      901 aaagccatga gcgtgagcag cctgagcagc ctcagctccc cctcctttac cgagtcacag
      961 ccaattgatt ttggggcaat gccatatgta tgtgatgagt gtgggaggtc gttcagtgtc
     1021 atctcagaat ttgttgagca ccagatcatg catactagag agaacctcta tgagtatggt
     1081 gagtccttta tccacagtgt ggctgtcagt gaagttcaga aaagtcaggt tggagggaaa
     1141 cgttttgaat gtaaggactg tggagagacc ttcaataaga gtgccgcctt ggctgaacat
     1201 cggaagattc atgctagagg ttatcttgtg gaatgtaaga atcaggaatg tgaggaagcc
     1261 ttcatgccta gccccacctt tagtgagctt cagaaaatat atggcaaaga caaattctac
     1321 gagtgcaggg tgtgtaagga aaccttcctt catagttctg ccctgattga gcaccagaaa
     1381 atccactttg gggatgacaa agataatgag cgtgaacatg aacgtgaacg tgaacgtgag
     1441 cgcggggaaa cctttaggcc cagcccagcc cttaatgagt ttcagaaaat gtatggtaaa
     1501 gagaaaatgt acgaatgtaa ggtgtgtggg gagactttcc ttcatagctc atccctgaaa
     1561 gaacatcaga aaatccatac tagagggaac ccatttgaaa acaagggtaa agtgtgtgag
     1621 gaaaccttta ttcctggtca gtcccttaaa aggcgtcaga aaacttacaa taaggagaag
     1681 ctctgtgact ttacagatgg ccgggatgcc ttcatgcaaa gctcagagct cagtgagcat
     1741 cagaaaattc attctcgaaa gaacctcttt gaaggcagag ggtatgagaa atctgtcatt
     1801 catagtgggc cattcactga atctcagaag agtcatacta taacaagacc tcttgaaagt
     1861 gatgaggacg aaaaggcgtt caccattagc tctaacccct atgaaaacca gaagattccc
     1921 actaaggaaa atgtctatga ggcaaaatca tatgagaggt ctgttattca tagcttagcc
     1981 tctgtggaag ctcagaaaag tcacagtgta gcagggccca gtaaaccaaa agtaatggca
     2041 gagtctacca ttcagagctt cgatgctatc aaccatcaga gagttcgtgc tggagggaac
     2101 acctctgaag gaagggaata cagtaggtct gttatccata gcttagtggc ttccaaacct
     2161 ccaagaagtc acaatggaaa tgaattggtg gaatctaatg agaagggaga atcctccatt
     2221 tatatctcag accttaatga taagcgacag aagattcctg ccagagagaa cccttgtgaa
     2281 gggggcagta agaatcgcaa ctatgaagac tctgtcatac agagtgtatt ccgtgccaaa
     2341 cctcagaaaa gtgttcctgg agagggatct ggtgagttta agaaggatgg cgaattctct
     2401 gttcccagct caaatgtccg tgaataccag aaggctcgtg ctaaaaagaa atacattgag
     2461 cataggagca atgagacctc tgtaattcac tctctgcctt ttggtgaaca aacatttcgc
     2521 cctcgaggga tgctctatga atgtcaggag tgtggggagt gctttgctca tagctctgac
     2581 ctcactgagc accagaagat tcatgatagg gagaagccct ctggaagcag aaactatgaa
     2641 tggtctgtca ttcgcagctt ggcccctact gaccctcaaa caagttacgc ccaagagcag
     2701 tatgctaaag agcaagcgcg gaacaaatgt aaggacttca gacaattttt tgctaccagc
     2761 gaagacctca acacaaacca gaaaatctat gaccaagaga agtctcatgg cgaggagtct
     2821 caaggcgaga atactgatgg ggaggagacc cacagcgagg agacccatgg tcaggagaca
     2881 attgaagacc ctgtcattca aggctcagac atggaagacc ctcagaagga tgaccctgat
     2941 gacaaaatct atgaatgtga ggactgtggc ctgggctttg tggatctcac agacctcaca
     3001 gaccatcaga aagtccacag caggaagtgc ctggttgaca gtcgggagta cacacattct
     3061 gtaattcaca cccattccat cagcgagtat cagagagatt acactggaga gcagctgtat
     3121 gaatgtccaa agtgtgggga atcttttatt catagctcat tccttttcga gcatcagaga
     3181 atccatgaac aagaccagtt gtattccatg aaggggtgtg atgatggttt tattgccctc
     3241 ttgcccatga agccacggag gaatcgtgct gcagagagga atcctgctct tgctgggtcg
     3301 gccattcgat gccttttgtg tggacaaggc ttcattcata gctctgccct taatgagcat
     3361 atgagacttc atagggaaga tgatttactg gagcagagcc agatggctga ggaagctatc
     3421 attccaggct tagccctcac tgagtttcag agaagtcaga ccgaagagag actctttgaa
     3481 tgtgcagtct gtggagaatc tttcgtcaac ccagcagaac ttgcagatca cgtaactgtt
     3541 cataagaatg agccctatga gtacgggtcc tcctatactc acacctcatt tcttactgag
     3601 cccctcaaag gagctatacc attctatgaa tgcaaggatt gtggtaagtc ctttattcat
     3661 agcacagtcc tcactaaaca taaggagctt catctggaag aagaagaaga agatgaagca
     3721 gcagcagctg cagcagcagc agcccaggaa gttgaagcca atgtccatgt tccacaagta
     3781 gttctgagga ttcagggctt aaacgtagag gctgctgagc cagaagtgga ggctgccgag
     3841 ccagaagtgg aggctgctga gccagaagtg gaggctgctg agccaaacgg agaggctgaa
     3901 gggccagatg gagaggctgc agagcccatt ggagaggctg gacagccaaa tggagaggcc
     3961 gagcagccaa atggggatgc tgatgagcca gatggtgcag gtattgaaga cccagaagaa
     4021 agagctgaag agccagaggg aaaagctgaa gagccagagg gagatgccga cgagcctgac
     4081 ggtgtgggaa ttgaagaccc agaagaaggt gaagatcaag agattcaggt agaagaacca
     4141 tactatgact gccatgaatg cacagaaacc ttcacttcca gcacagcatt cagtgaacac
     4201 ctgaaaactc atgccagcat gatcatattt gagcctgcaa atgcctttgg ggagtgctca
     4261 ggctacatcg aacgtgccag caccagcaca ggtggtgcca atcaagctga tgagaagtac
     4321 ttcaaatgtg acgtctgtgg gcagctcttc aatgaccgcc tgtccctcgc cagacaccag
     4381 aatacccaca ctggctagta atgaactagt ccgtaacttg aaagtatttc gatttcttgg
     4441 ctttatatat cttgtggaaa ggacgagcgt gcaataaacg acaagctcgg
//
