LOCUS       sequence-284325-        1661 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0504
FEATURES             Location/Qualifiers
     source          1..1661
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggctcgtga tttggtgatg ttcagagatg tggctgtaga cttttctcag
       61 gaagaatggg aatgcctgaa ctcatatcag aggaatttgt acagagatgt gatattagag
      121 aactatagca acttggtgtc actggcagga tgttctattt ctaagccaga tgtgatcacc
      181 ctattggaac aagggaaaga gccctggatg gttgtgaggg atgagaaaag aagatggact
      241 ctagatttgg aatccagata tgacactaaa aagttatttc aaggaaagga tatttatgaa
      301 atgaacttat ctcagtggaa ggtaatggaa agaattaaaa gctgtggact tgaagaacaa
      361 gaaagtcctc atgaggtgtg tttcaggcaa gtgacaaaaa ccacctctga aaaaatgcct
      421 acttacagaa aactcacatc tcttcctctg tatcagaaaa gtcataacag agagaaaccc
      481 tacgaatgtg gggaatgtgg gaaggctttt agagtacgac aacaacttac tttccatcaa
      541 agaattcata ctggtgagaa accctatgaa tgtaaagaat gtggaaaagc ctttagacag
      601 tgtgcccacc tcagtcgaca tcagagaatt catacttctg acaaactcta tgaatgtaaa
      661 aaatgtggaa agatcttcac atgtggctca gaccttcgag tacatcagag aattcatatt
      721 ggtgagaagc cgtatgaatg taaagaatgt gggaaagcct ttagagttag aggacaactt
      781 aatctccatc aaaggattca cacgggtgag aaaccctatg aatgtaaaga atgtgggaag
      841 gcctttaggc agtatgcaca ccttactcga catcagagac tgaacattgc tgagaagtgc
      901 tatgagtgta aggaatgtgg tcaggccttt ctgtgtagta caggccttcg actacatcac
      961 aaacttcata ctggagaaaa accctatgaa tgtaaggagt gtggaaaggc ctttagagtg
     1021 cggcagcaac ttactctcca tcagagaatt cacactggtg agaagcctta tgattgtaag
     1081 gaatgtggga agactttcag tcgtggctat catctaactc tccatcagag aatacatact
     1141 ggtgaaaagc cctatgaatg taaggaatgt cagaagttct ttcgtcgtta ctcagaactt
     1201 atttcacatc agggtattca cattggagag aaaccttatg aatgtaagga atgtgggaag
     1261 gcatttaggc tgttctcaca gcttactcaa catcagagta ttcattttgg tgagaaaccc
     1321 tttaagtgta aggaatgtga gaagactttt agactgcttt cacaacttac ccaacatcaa
     1381 agtattcata ctggtgaaaa gccctatgac tgtaaggaat gtggaaaggc ctttagactt
     1441 cattcatcac tgattcaaca tcagagaatt cattctggtg agaaaccata caaatgtaag
     1501 gaatgtaaaa aggcatttag acaacattca catcttactt accatcagcg aattcataat
     1561 gtaacttagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     1621 tcttgtggaa aggacgaaca aacgctttgg aggattcaga c
//
