LOCUS       sequence-284340-        7478 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7478)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 7478)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0703
FEATURES             Location/Qualifiers
     source          1..7478
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 accgccacca tgtcgggatc cacacagcct gtggcacaga cgtggagggc cactgagccc
       61 cgctacccgc cccacagcct ttcctaccca gtgcagatcg cccggacgca cacggacgtc
      121 gggctcctgg agtaccagca ccactcccgc gactatgcct cccacctgtc gcccggctcc
      181 atcatccagc cccagcggcg gaggccctcc ctgctgtctg agttccagcc cgggaatgaa
      241 cggtcccagg agctccacct gcggccagag tcccactcat acctgcccga gctggggaag
      301 tcagagatgg agttcattga aagcaagcgc cctcggctag agctgctgcc tgaccccctg
      361 ctgcgaccgt cacccctgct ggccacgggc cagcctgcgg gatctgaaga cctcaccaag
      421 gaccgtagcc tgacgggcaa gctggaaccg gtgtctcccc ccagcccccc gcacactgac
      481 cctgagctgg agctggtgcc gccacggctg tccaaggagg agctgatcca gaacatggac
      541 cgcgtggacc gagagatcac catggtagag cagcagatct ctaagctgaa gaagaagcag
      601 caacagctgg aggaggaggc tgccaagccg cccgagcctg agaagcccgt gtcaccgccg
      661 cccatcgagt cgaagcaccg cagcctggtg cagatcatct acgacgagaa ccggaagaag
      721 gctgaagctg cacatcggat tctggaaggc ctggggcccc aggtggagct gccgctgtac
      781 aaccagccct ccgacacccg gcagtatcat gagaacatca aaataaacca ggcgatgcgg
      841 aagaagctaa tcttgtactt caagaggagg aatcacgctc ggaaacaatg ggagcagaag
      901 ttctgccagc gctatgacca gctcatggag gcctgggaga agaaggtgga gcgcatcgag
      961 aacaaccccc ggcggcgggc caaggagagc aaggtgcgcg agtactacga gaagcagttc
     1021 cctgagatcc gcaagcagcg cgagctgcag gagcgcatgc agagggtggg ccagcggggc
     1081 agtgggctgt ccatgtcggc cgcccgcagc gagcacgagg tgtcagagat catcgatggc
     1141 ctctcagagc aggagaacct ggagaagcag atgcgccagc tggccgtgat cccgcccatg
     1201 ctgtacgacg ctgaccagca gcgcatcaag ttcatcaaca tgaacgggct tatggccgac
     1261 cccatgaagg tgtacaaaga ccgccaggtc atgaacatgt ggagtgagca ggagaaggag
     1321 accttccggg agaagttcat gcagcatccc aagaactttg gcctgatcgc atcattcctg
     1381 gagaggaaga cagtggctga gtgcgtcctc tattactacc tgactaagaa gaatgagaac
     1441 tataagagcc tggtgagacg gagctatcgg cgccgcggca agagccagca gcagcaacaa
     1501 cagcagcagc agcagcagca gcagcagcag cagcagccca tgccccgcag cagccaggag
     1561 gagaaagatg agaaggagaa ggaaaaggag gcggagaagg aggaggagaa gccggaggtg
     1621 gagaacgaca aggaagacct cctcaaggag aagacagacg acacctcagg ggaggacaac
     1681 gacgagaagg aggctgtggc ctccaaaggc cgcaaaactg ccaacagcca gggaagacgc
     1741 aaaggccgca tcacccgctc aatggctaat gaggccaaca gcgaggaggc catcaccccc
     1801 cagcagagcg ccgagctggc ctccatggag ctgaatgaga gttctcgctg gacagaagaa
     1861 gaaatggaaa cagccaagaa aggtctcctg gaacacggcc gcaactggtc ggccatcgcc
     1921 cggatggtgg gctccaagac tgtgtcgcag tgtaagaact tctacttcaa ctacaagaag
     1981 aggcagaacc tcgatgagat cttgcagcag cacaagctga agatggagaa ggagaggaac
     2041 gcgcggagga agaagaagaa agcgccggcg gcggccagcg aggaggctgc attcccgccc
     2101 gtggtggagg atgaggagat ggaggcgtcg ggcgtgagcg gaaatgagga ggagatggtg
     2161 gaggaggctg aagccactgt caacaacagc tcagacaccg agagcatccc ctctcctcac
     2221 actgaggccg ccaaggacac agggcagaat gggcccaagc ccccagccac cctgggcgcc
     2281 gacgggccac ccccagggcc acccacccca ccaccggagg acatcccggc ccccactgag
     2341 cccaccccgg cctctgaagc caccggagcc cctacgcccc caccagcacc cccatcgccc
     2401 tctgcacctc ctcctgtggt ccccaaggag gagaaggagg aggagaccgc agcagcgccc
     2461 ccagtggagg agggggagga gcagaagccc cccgcggctg aggagctggc agtggacaca
     2521 gggaaggccg aggagcccgt caagagcgag tgcacggagg aagccgagga ggggccggcc
     2581 aagggcaagg acgcggaggc cgctgaggcc acggccgagg gggcgctcaa ggcagagaag
     2641 aaggagggcg ggagcggcag ggccaccaca gccaagagct cgggcgcccc ccaggacagc
     2701 gactccagtg ctacctgcag tgcagacgag gtggatgagg ccgagggcgg cgacaagaac
     2761 cggctgctgt ccccaaggcc cagcctcctc accccgactg gcgacccccg ggccaatgcc
     2821 tcaccccaga agccactgga cctgaagcag ctgaagcagc gagcggctgc catccccccc
     2881 atccaggtca ccaaagtcca tgagcccccc cgggaggacg cagctcccac caagccagct
     2941 cccccagccc caccgccacc gcaaaacctg cagccggaga gcgacgcccc tcagcagcct
     3001 ggcagcagcc cccggggcaa gagcaggagc ccggcacccc ccgccgacaa ggaggcagag
     3061 aagcctgtgt tcttcccagc cttcgcagcc gaggcccaga agctgcctgg ggacccccct
     3121 tgctggactt ccggcctgcc cttccccgtg cccccccgtg aggtgatcaa ggcctccccg
     3181 catgccccgg acccctcagc cttctcctac gctccacctg gtcacccact gcccctgggc
     3241 ctccatgaca ctgcccggcc cgtcctgccg cgcccaccca ccatctccaa cccgcctccc
     3301 ctcatctcct ctgccaagca ccccagcgtc ctcgagaggc aaataggtgc catctcccaa
     3361 ggaatgtcgg tccagctcca cgtcccgtac tcagagcatg ccaaggcccc ggtgggccct
     3421 gtcaccatgg ggctgcccct gcccatggac cccaaaaagc tggcaccctt cagcggagtg
     3481 aagcaggagc agctgtcccc acggggccag gctgggccac cggagagcct gggggtgccc
     3541 acagcccagg aggcgtccgt gctgagaggg acagctctgg gctcagttcc gggcggaagc
     3601 atcaccaaag gcattcccag cacacgggtg ccctcggaca gcgccatcac ataccgcggc
     3661 tccatcaccc acggcacgcc agctgacgtc ctgtacaagg gcaccatcac caggatcatc
     3721 ggcgaggaca gcccgagtcg cttggaccgc ggccgggagg acagcctgcc caagggccac
     3781 gtcatctacg aaggcaagaa gggccacgtc ttgtcctatg agggtggcat gtctgtgacc
     3841 cagtgctcca aggaggacgg cagaagcagc tcaggacccc cccatgagac ggccgccccc
     3901 aagcgcacct atgacatgat ggagggccgc gtgggcagag ccatctcctc agccagcatc
     3961 gaaggtctca tgggccgtgc catcccgccg gagcgacaca gcccccacca cctcaaagag
     4021 cagcaccaca tccgcgggtc catcacacaa gggatccctc ggtcctacgt ggaggcacag
     4081 gaggactacc tgcgtcggga ggccaagctc ctaaagcggg agggcacgcc tccgccccca
     4141 ccgccctcac gggacctgac cgaggcctac aagacgcagg ccctgggccc cctgaagctg
     4201 aagccggccc atgagggcct ggtggccacg gtgaaggagg cgggccgctc catccatgag
     4261 atcccgcgcg aggagctgcg gcacacgccc gagctgcccc tggccccgcg gccgctcaag
     4321 gagggctcca tcacgcaggg caccccgctc aagtacgaca ccggcgcgtc caccactggc
     4381 tccaaaaagc acgacgtacg ctccctcatc ggcagccccg gccggacgtt cccacccgtg
     4441 cacccgctgg atgtgatggc cgacgcccgg gcactggaac gtgcctgcta cgaggagagc
     4501 ctgaagagcc ggccagggac cgccagcagc tcggggggct ccattgcgcg cggcgccccg
     4561 gtcattgtgc ctgagctggg taagccgcgg cagagccccc tgacctatga ggaccacggg
     4621 gcaccctttg ccggccacct cccacgaggt tcgcccgtga ccacgcggga gcccacgccg
     4681 cgcctgcagg agggcagcct ttcgtccagc aaggcatccc aggaccgaaa gctgacgtcg
     4741 acgcctcgtg agatcgccaa gtccccgcac agcaccgtgc ccgagcacca cccacacccc
     4801 atctcgccct atgagcacct gcttcggggc gtgagtggcg tggacctgta tcgcagccac
     4861 atccccctgg ccttcgaccc cacctccata ccccgcggca tccctctgga cgcagccgct
     4921 gcctactacc tgccccgaca cctggccccc aaccccacct acccgcacct gtacccaccc
     4981 tacctcatcc gcggctaccc cgacacggcg gcgctggaga accggcagac catcatcaat
     5041 gactacatca cctcgcagca gatgcaccac aacgcggcca ccgccatggc ccagcgagct
     5101 gatatgctga ggggcctctc gccccgcgag tcctcgctgg cactcaacta cgctgcgggt
     5161 ccccgaggca tcatcgacct gtcccaagtg ccacacctgc ctgtgctcgt gcccccgaca
     5221 ccaggcaccc cagccaccgc catggaccgc cttgcctacc tccccaccgc gccccagccc
     5281 ttcagcagcc gccacagcag ctccccactc tccccaggag gtccaacaca cttgacaaaa
     5341 ccaaccacca cgtcctcgtc cgagcgggag cgagaccggg atcgagagcg ggaccgggat
     5401 cgggagcggg aaaagtccat cctcacgtcc accacgacgg tggagcacgc acccatctgg
     5461 agacctggta cagagcagag cagcggcagc agcggcgggg gtgggggcag cagcagccgc
     5521 cccgcctccc actcccatgc ccaccagcac tcgcccatct cccctcggac ccaggatgcc
     5581 ctccagcaga gacccagtgt gcttcacaac acaggcatga agggtatcat caccgctgtg
     5641 gagcccagca cgcccacggt cctgaggtcc acctccacct cctcacccgt tcgcccggct
     5701 gccacattcc cacctgccac ccactgccca ctgggcggca ccctcgatgg ggtctaccct
     5761 accctcatgg agcccgtctt gctgcccaag gaggcccccc gggtcgcccg gccagagcgg
     5821 ccccgagcag acaccggcca tgccttcctc gccaagcccc cagcccgctc cgggctggag
     5881 cccgcctcct cccccagcaa gggctcggag ccccggcccc tagtgcctcc tgtctctggc
     5941 cacgccacca tcgcccgcac ccctgcgaag aacctcgcac ctcaccacgc cagcccggac
     6001 ccgccggcgc cacctgcctc ggcctcggac ccgcaccggg aaaagactca aagtaaaccc
     6061 ttttccatcc aggaactgga actccgttct ctgggttacc acggcagcag ctacagcccc
     6121 gaaggggtgg agcccgtcag ccctgtgagc tcacccagtc tgacccacga caaggggctc
     6181 cccaagcacc tggaagagct cgacaagagc cacctggagg gggagctgcg gcccaagcag
     6241 ccaggccccg tgaagcttgg cggggaggcc gcccacctcc cacacctgcg gccgctgcct
     6301 gagagccagc cctcgtccag cccgctgctc cagaccgccc caggggtcaa aggtcaccag
     6361 cgggtggtca ccctggccca gcacatcagt gaggtcatca cacaggacta cacccggcac
     6421 cacccacagc agctcagcgc acccctgccc gcccccctct actccttccc tggggccagc
     6481 tgccccgtcc tggacctccg ccgcccaccc agtgacctct acctcccgcc cccggaccat
     6541 ggtgccccgg cccgtggctc cccccacagc gaagggggca agaggtctcc agagccaaac
     6601 aagacgtcgg tcttgggtgg tggtgaggac ggtattgaac ctgtgtcccc accggagggc
     6661 atgacggagc cagggcactc ccggagtgct gtgtacccgc tgctgtaccg ggatggggaa
     6721 cagacggagc ccagcaggat gggctccaag tctccaggca acaccagcca gccgccagcc
     6781 ttcttcagca agctgaccga gagcaactcc gccatggtca agtccaagaa gcaagagatc
     6841 aacaagaagc tgaacaccca caaccggaat gagcctgaat acaatatcag ccagcctggg
     6901 acggagatct tcaatatgcc cgccatcacc ggaacaggcc ttatgaccta tagaagccag
     6961 gcggtgcagg aacatgccag caccaacatg gggctggagg ccataattag aaaggcactc
     7021 atgggtggcg gcgggaaggc caaggtctct ggcagaccca gcagccgaaa agccaagtcc
     7081 ccggccccgg gcctggcatc tggggaccgg ccaccctctg tctcctcagt gcactcggag
     7141 ggagactgca accgccggac gccgctcacc aaccgcgtgt gggaggacag gccctcgtcc
     7201 gcaggttcca cgccattccc ctacaacccc ctgatcatgc ggctgcaggc gggtgtcatg
     7261 gcttccccac ccccaccggg cctccccgcg ggcagcgggc ccctcgctgg cccccaccac
     7321 gcctgggacg aggagcccaa gccactgctc tgctcgcagt acgagacact ctccgacagc
     7381 gagtagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
     7441 tgtggaaagg acgatgcata ggccttgtat tgcttgtt
//
