LOCUS       sequence-284349-        1010 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1038
FEATURES             Location/Qualifiers
     source          1..1010
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             333..344
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 accgccacca tggagaaggg ttacagcacg gtcaccttcg acgggacgcc cagctacggt
       61 cacacgccct cgcaccatgc ggcgcagttc cccaaccact cattcaagca tgaggatccc
      121 atgggccagc agggctcgct gggtgagcag cagtactcgg tgccgccccc ggtctatggc
      181 tgccacaccc ccaccgacag ctgcaccggc agccaggctt tgctgctgag gacgccctac
      241 agcagtgaca atttatacca aatgacatcc cagcttgaat gcatgacctg gaatcagatg
      301 aacttaggag ccaccttaaa gggagttgct gctgggagct ccagctcagt gaaatggaca
      361 gaagggcaga gcaaccacag cacagggtac gagagcgata accacacaac gcccatcctc
      421 tgcggagccc aatacagaat acacacgcac ggtgtcttca gaggcattca ggatgtgcga
      481 cgtgtgcctg gagtagcccc gactcttgta cggtcggcat ctgagaccag tgagaaacgc
      541 cccttcatgt gtgcttaccc aggctgcaat aagagatatt ttaagctgtc ccacttacag
      601 atgcacagca ggaagcacac tggtgagaaa ccataccagt gtgacttcaa ggactgtgaa
      661 cgaaggtttt ctcgttcaga ccagctcaaa agacaccaaa ggagacatac aggtgtgaaa
      721 ccattccagt gtaaaacttg tcagcgaaag ttctcccggt ccgaccacct gaagacccac
      781 accaggactc atacaggtga aaagcccttc agctgtcggt ggccaagttg tcagaaaaag
      841 tttgcccggt cagatgaatt agtccgccat cacaacatgc atcagagaaa catgaccaaa
      901 ctccagctgg cgctttagta atgaactagt ccgtaacttg aaagtatttc gatttcttgg
      961 ctttatatat cttgtggaaa ggacgatcac cgtcaagtag tcagtagatg
//
