LOCUS       sequence-284352-        1898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1050
FEATURES             Location/Qualifiers
     source          1..1898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             602..610
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 accgccacca tgctggagac ttataaccat ctggtctcac tggaaattcc atcttctaaa
       61 ccaaaactca ttgctcagct ggagcgaggg gaagcgccct ggagagagga gagaaaatgt
      121 ccactggacc tctgtccaga atcgaagcca gaaattcaac ttagtccctc ctgccctctg
      181 attttctcca gtcagcaagc tctcagccaa catgtgtggc tgagtcatct ctctcagctg
      241 ttttcaagtt tatgggcagg aaatcctctc cacctgggaa aacactatcc agaagatcag
      301 aaacaacagc aggatccatt ctgctttagt ggcaaagcag aatggattca agagggagaa
      361 gactccagac tcctgtttgg gagagtaagc aaaaatggca cttcaaaggc actttccagc
      421 ccacctgaag aacaacagcc agcacagtcc aaggaagaca acacagtggt ggatataggg
      481 tccagccctg aacggagggc agatctagag gaaacagaca aagtattgca tggtttagaa
      541 gtctcaggat ttggagaaat caaatatgaa gagtttgggc caggctttat caaggagtca
      601 aacctcctta gcctccagaa gacacaaact ggggagacac cttacatgta cactgagtgg
      661 ggagacagct ttggcagtat gtcagtcctc atcaaaaacc caaggacaca ctctggggga
      721 aagccttatg tgtgcaggga atgtgggcga ggctttacgt ggaagtcaaa cctgatcaca
      781 catcagagga cacactcagg ggagaaacct tatgtgtgca aggattgtgg acgaggcttt
      841 acttggaagt cgaacctctt tacacatcag cggacacact cagggctcaa gccttatgtg
      901 tgcaaggaat gtgggcagag ctttagcctg aagtcaaacc tcattaccca ccagagggcg
      961 cacactgggg agaagcctta tgtttgcagg gaatgtgggc gtggctttcg ccagcattca
     1021 cacctggtca gacacaagag gacacattca ggagagaagc cttacatttg cagggagtgt
     1081 gagcaaggct ttagccagaa gtcacacctc atcagacact taaggacaca cacaggagag
     1141 aagccttatg tatgcacaga atgtgggcgt cactttagct ggaaatcaaa cctcaaaaca
     1201 caccagagga cacactcagg ggttaaacct tatgtctgcc tggagtgcgg gcagtgcttt
     1261 agcctgaagt caaaccttaa caaacaccag aggtcacaca cgggggagaa gccatttgta
     1321 tgtacggagt gtgggcgagg ctttacccgg aaatcaaccc tgagcacgca ccagaggaca
     1381 cactcagggg agaagccatt tgtatgtgct gagtgtggac gaggctttaa tgataagtcc
     1441 accctcattt cacaccagag gacacattca ggggaaaagc cttttatgtg cagggagtgt
     1501 ggcagaaggt ttcggcagaa gcctaacctg tttaggcaca agagggcaca ctcaggtgcc
     1561 tttgtgtgca gggagtgtgg gcaaggcttt tgtgctaagt taactctcat taaacaccag
     1621 agagcacacg caggggggaa gcctcatgtg tgcagggagt gtgggcaagg ctttagccgg
     1681 cagtcacacc tcattagaca ccagaggaca cattcaggag agaagcctta tatttgcaga
     1741 aagtgtggac ggggctttag tcggaagtcc aaccttatca gacatcagag gacacactca
     1801 ggatagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
     1861 tgtggaaagg acgaccctga aggtggcctt cgtatagg
//
