LOCUS       sequence-284358-        2291 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1120
FEATURES             Location/Qualifiers
     source          1..2291
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggaaaaaa atacaagaac attgttctac taaaaggatt agaggtcatc
       61 aatgattatc attttagaat ggttaagtcc ttactgagca acgatttaaa acttaattta
      121 aaaatgagag aagagtatga caaaattcag attgctgact tgatggaaga aaagttccga
      181 ggtgatgctg gtttgggcaa actaataaaa attttcgaag atataccaac gcttgaagac
      241 ctggctgaaa ctcttaaaaa agaaaagtta aaagtaaaag gaccagccct atcaagaaag
      301 aggaagaagg aagtggatgc tacttcacct gcaccctcca caagcagcac tgtcaaaact
      361 gaaggagcag aggcaactcc tggagctcag aacccgaaaa cagtggccaa atgtcaggta
      421 actcccagaa gaaatgttct ccaaaaacgc ccagtgatag tgaaggtact gagtacaaca
      481 aagccatttg aatatgagac cccagaaatg gagaaaaaaa taatgtttca tgctacagtg
      541 gctacacaga cacagttctt ccatgtgaag gttttaaaca ccagcttgaa ggagaaattc
      601 aatggaaaga aaatcatcat catatcagat tatttggaat atgatagtct cctagaggtc
      661 aatgaagaat ctactgtatc tgaagctggt cctaaccaaa cgtttgaggt tccaaataaa
      721 atcatcaaca gagcaaagga aactctgaag attgatattc ttcacaaaca agcttcagga
      781 aatattgtat atggggtatt tatgctacat aagaaaacag taaatcagaa gaccacaatc
      841 tacgaaattc aggatgatag aggaaaaatg gatgtagtgg ggacaggaca atgtcacaat
      901 atcccctgtg aagaaggaga taagctccaa cttttctgct ttcgacttag aaaaaagaac
      961 cagatgtcaa aactgatttc agaaatgcat agttttatcc agataaagaa aaaaacaaac
     1021 ccgagaaaca atgaccccaa gagcatgaag ctaccccagg aacagcgtca gcttccatat
     1081 ccttcagagg ccagcacaac cttccctgag agccatcttc ggactcctca gatgccacca
     1141 acaactccat ccagcagttt cttcaccaag aaaagtgaag acacaatctc caaaatgaat
     1201 gacttcatga ggatgcagat actgaaggaa gggagtcatt ttccaggacc gttcatgacc
     1261 agcataggcc cagctgagag ccatccccac actcctcaga tgcctccatc aacaccaagc
     1321 agcagtttct taaccacgaa aagtgaagac acaatctcca aaatgaatga cttcatgagg
     1381 atgcagatac tgaaggaagg gagtcatttt ccaggaccgt tcatgaccag cataggccca
     1441 gctgagagcc atccccacac tcctcagatg cctccatcaa caccaagcag cagtttctta
     1501 accacgttga aaccaagact gaagactgaa cctgaagaag tttccataga agacagtgcc
     1561 cagagtgacc tcaaagaagt gatggtgctg aacgcaacag aatcatttgt atatgagccc
     1621 aaagagcaga agaaaatgtt tcatgccaca gtggcaactg agaatgaagt cttccgagtg
     1681 aaggttttta atattgacct aaaggagaag ttcaccccaa agaagatcat tgccatagca
     1741 aattatgttt gccgcaatgg gttcctggag gtatatcctt tcacacttgt ggctgatgtg
     1801 aatgctgacc gaaacatgga gatcccaaaa ggattgatta gaagtgccag cgtaactcct
     1861 aaaatcaatc agctttgctc acaaactaaa ggaagttttg tgaatggggt gtttgaggta
     1921 cataagaaaa atgtaagggg tgaattcact tattatgaaa tacaagataa tacagggaag
     1981 atggaagtgg tggtgcatgg acgactgacc acaatcaact gtgaggaagg agataaactg
     2041 aaactcacct gctttgaatt ggcaccgaaa agtgggaata ccggggagtt gagatctgta
     2101 attcatagtc acatcaaggt catcaagacc aggaaaaaca agaaagacat actcaatcct
     2161 gattcaagta tggaaacttc accagacttt ttcttctagt aatgaactag tccgtaactt
     2221 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgattg tgaggttctt
     2281 cggcacaagg t
//
