LOCUS       sequence-284373-        4802 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4802)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 4802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1153
FEATURES             Location/Qualifiers
     source          1..4802
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             986..994
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 accgccacca tgacagcgga caaagacaaa gacaaagaca aagagaagga ccgggaccga
       61 gaccgggacc gagagagaga gaaaagagac aaagcaagag agagtgagaa ttcaaggcca
      121 cgccggagct gtaccttgga aggaggagcc aaaaattatg ctgagagtga tcacagtgaa
      181 gacgaggaca atgacaacaa tagtgccacc gcagaggagt ccacgaagaa gaataagaag
      241 aaaccaccga aaaaaaagtc tcgttatgaa aggacagata ccggtgagat aacatcctac
      301 atcactgaag atgatgtggt ctacagacca ggagactgtg tgtatatcga gagtcggagg
      361 ccaaacacac cgtatttcat ctgtagcatt caagacttca aactggtcca caactcccag
      421 gcctgttgca gatctccaac tcctgctttg tgtgaccccc cagcatgctc tctgccggtg
      481 gcatcacagc caccacagca tctttctgaa gccgggagag ggcctgtagg gagtaagagg
      541 gaccatctcc tcatgaacgt caaatggtac taccgtcaat ctgaggttcc agattctgtg
      601 tatcagcatt tggttcagga tcgacataat gaaaatgact ctggaagaga acttgtcatt
      661 acagacccag ttatcaagaa ccgagagctc ttcatttctg attacgttga cacttaccat
      721 gctgctgccc ttagagggaa gtgtaacatc tcccattttt ctgacatatt tgctgctaga
      781 gagtttaaag cccgagtgga ttcatttttc tacatattag gatataaccc tgagacaagg
      841 aggctgaaca gtacccaggg ggagattcgt gtcggtccta gtcatcaggc caaacttcca
      901 gatctgcaac catttccttc tccagatggt gatacagtga cccaacatga ggaactggtc
      961 tggatgcctg gagttaacga ctgtgacctc cttatgtact tgagggcagc aaggagcatg
     1021 gcggcatttg caggaatgtg tgatggaggc tctacagagg acggctgtgt cgcagcctct
     1081 cgggatgaca ccactctgaa tgcactgaac acactgcatg aaagcggtta cgatgctggc
     1141 aaagccctgc agcgcctggt gaagaagcct gtgcccaagc tcatcgagaa gtgctggacc
     1201 gaggacgaag tgaaacgctt cgttaaggga ctcaggcagt acgggaagaa cttcttcaga
     1261 attagaaagg agctgcttcc caataaggaa acaggggagc tgatcacctt ctattactat
     1321 tggaagaaga cccccgaagc agccagctcc cgagcccatc gtaggcaccg caggcaggcc
     1381 gtgttcagga ggattaagac tcgcaccgcg tccacacccg tcaacacacc ctccagaccc
     1441 ccgtccagtg aattcttgga cctaagttca gccagtgaag atgacttcga cagtgaggac
     1501 agtgagcagg agctgaaggg gtacgcctgc cgccactgct tcaccaccac ctccaaagat
     1561 tggcaccacg gaggccggga gaacatcctg ctttgcaccg actgtcgcat ccacttcaag
     1621 aaatacggtg agctcccgcc cattgagaag cccgtggacc cgccaccgtt tatgttcaaa
     1681 cccgtcaagg aagaggatga tgggctcagt gggaagcata gcatgaggac acggcggagt
     1741 cggggctcga tgtcgacact acgcagtggt cggaagaagc agccagccag ccctgatggt
     1801 cgcacctcac ccatcaatga agacatccgc tccagcggcc ggaactcccc cagcgctgcc
     1861 agtacctcca gcaatgacag taaagcagag acagtgaaga agtcggccaa gaaggtgaag
     1921 gaggaagcct cttcccctct taagagtaac aaacgccagc gggagaaggt ggcctctgat
     1981 acggaggagg ctgacaggac cagctccaag aagacaaaaa cgcaggagat cagcaggccc
     2041 aactcgccat ctgaaggtga gggagagagt tcagacagtc gcagcgtcaa cgatgagggt
     2101 agcagtgacc ccaaagacat cgaccaggac aatcgcagca cgtccccgag catccccagc
     2161 ccccaggaca atgagagtga ctcggactcg tcagcccagc agcagatgct gcaggcccag
     2221 cccccagcct tgcaggctcc cactggggtc accccagctc cctcctcagc tcctccaggg
     2281 acccctcagc tgcccacgcc agggcccacg ccctctgcca ctgcagttcc cccacagggc
     2341 tcccccacgg cctcccaggc ccctaaccag ccacaggctc ccacagcgcc tgttccccac
     2401 acccacatcc aacaggcacc ggccttgcac ccccagcggc cgccctcacc gcatcccccg
     2461 ccgcatccct cgccacatcc cccgctgcag cctctgactg ggtcggcggg ccagccttct
     2521 gcaccctctc atgcccagcc cccactgcac ggtcagggcc cacccggccc tcacagcctg
     2581 caggctgggc ccctgctgca gcacccaggc cccccacagc cctttggcct ccctccccag
     2641 gcctcccaag gccaggcccc tctggggacc tccccagcag cagcgtaccc tcacacctcc
     2701 ctgcagctgc cagcctctca gtcagcgctg cagtcccaac agcctccacg ggagcagccc
     2761 ctgccaccag cgcccttggc catgccccac atcaagcccc cgcctaccac tcccatcccc
     2821 cagctgccgg cgccacaggc ccacaagcac cctccccacc tctcggggcc ctcacccttc
     2881 tccatgaatg ccaacctgcc tccccctcca gccctgaagc ccctgagctc cctgtccaca
     2941 catcaccccc cgtcggctca ccccccaccc ctgcaactca tgcctcagag ccagccattg
     3001 ccctcctcgc ccgcccagcc ccccgggctg acccagagcc agaacctgcc cccgccccct
     3061 gcctcccacc cccctacagg cctccaccag gtggcccccc aacccccgtt tgctcagcac
     3121 ccctttgtcc ctggaggccc tcctcccatc acccctccga cctgcccctc cacctctacc
     3181 ccaccggcgg gacctggcac ctcggcccag ccaccctgct ctggtgcggc ggcttcagga
     3241 ggcagcatag cgggggggtc gtcctgccca ctccccaccg tccagatcaa ggaggaggct
     3301 ctggacgacg ctgaggagcc tgagagcccc cctcccccac caaggagccc gtccccggag
     3361 cccactgtgg tggacacccc cagtcacgcc agccagtcag ctaggttcta caaacacctg
     3421 gaccggggct acaactcgtg tgcccggaca gacctgtact tcatgcctct ggccgggtcc
     3481 aagctggcca agaagaggga ggaggccatt gagaaggcca agcgcgaggc tgagcagaaa
     3541 gcccgagagg agcgagagcg ggagaaggag aaggagaagg agcgggagcg ggagcgagag
     3601 cgggagcgcg aggcagagcg ggcggctaag gcgtccagct cagcgcatga aggtcgcctc
     3661 agtgacccac agctcagtgg tcctggccac atgcggccat ccttcgagcc accaccaacc
     3721 accattgctg ctgtgccccc ctacatcggg cccgacacac ctgcccttcg gactctgagc
     3781 gagtacgccc ggccccacgt catgtcgccc accaaccgca accacccctt ctacatgccc
     3841 cttaacccca cggaccccct gctggcctac cacatgcctg gcctctacaa cgtcgacccc
     3901 accatccgcg agcgggagct ccgggagcgg gagatccgag agcgggagat ccgagagcgg
     3961 gagctgcggg agaggatgaa gccgggcttc gaggtgaagc ccccagagct ggaccccctg
     4021 cacccagccg ccaaccccat ggagcacttt gcccggcaca gcgccctcac catccccccg
     4081 accgccgggc cccacccttt tgcttctttc cacccgggcc tgaacccctt ggagagggag
     4141 agactggccc tggcgggccc ccagctgcgg cccgagatga gctaccctga cagactggca
     4201 gccgagcgta tccacgcaga gcgcatggca tcgctgacca gcgatcccct ggcccgactg
     4261 cagatgttca acgtgactcc gcaccatcac cagcactctc acattcactc ccacctccac
     4321 ctccaccagc aggaccccct ccaccaaggt tcagcaggcc ccgttcaccc gctggtcgac
     4381 cccctgactg ccggtcccca cctggctcgc ttcccctacc cgcctggcac tctccccaac
     4441 cctctgcttg gacagccccc acacgagcac gagatgcttc gccacccagt tttcggcacc
     4501 ccctaccccc gtgacctgcc tggggccatc ccacccccca tgtcagcagc ccaccagctg
     4561 caggccatgc atgcccagtc ggccgagctg cagagactgg ccatggagca gcagtggctg
     4621 catggacacc cccacatgca tggtggccac ctaccaagtc aggaagatta ttacagtcga
     4681 ctgaagaaag aaggtgacaa gcagttatag taatgaacta gtccgtaact tgaaagtatt
     4741 tcgatttctt ggctttatat atcttgtgga aaggacgaag acagattccg aaagtctaca
     4801 gc
//
