LOCUS       sequence-284376-        3998 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3998)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1155
FEATURES             Location/Qualifiers
     source          1..3998
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     RBS             182..190
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 accgccacca tggatgatcc attcagcccc tgcaggaggc tgaacagtac ccagggggag
       61 attcgtgtcg gtcctagtca tcaggccaaa cttccagatc tgcaaccatt tccttctcca
      121 gatggtgata cagtgaccca acatgaggaa ctggtctgga tgcctggagt taacgactgt
      181 gacctcctta tgtacttgag ggcagcaagg agcatggcgg catttgcagg aatgtgtgat
      241 ggaggctcta cagaggacgg ctgtgtcgca gcctctcggg atgacaccac tctgaatgca
      301 ctgaacacac tgcatgaaag cggttacgat gctggcaaag ccctgcagcg cctggtgaag
      361 aagcctgtgc ccaagctcat cgagaagtgc tggaccgagg acgaagtgaa acgcttcgtt
      421 aagggactca ggcagtacgg gaagaacttc ttcagaatta gaaaggagct gcttcccaat
      481 aaggaaacag gggagctgat caccttctat tactattgga agaagacccc cgaagcagcc
      541 agctcccgag cccatcgtag gcaccgcagg caggccgtgt tcaggaggat taagactcgc
      601 accgcgtcca cacccgtcaa cacaccctcc agacccccgt ccagtgaatt cttggaccta
      661 agttcagcca gtgaagatga cttcgacagt gaggacagtg agcaggagct gaaggggtac
      721 gcctgccgcc actgcttcac caccacctcc aaagattggc accacggagg ccgggagaac
      781 atcctgcttt gcaccgactg tcgcatccac ttcaagaaat acggtgagct cccgcccatt
      841 gagaagcccg tggacccgcc accgtttatg ttcaaacccg tcaaggaaga ggatgatggg
      901 ctcagtggga agcatagcat gaggacacgg cggagtcggg gctcgatgtc gacactacgc
      961 agtggtcgga agaagcagcc agccagccct gatggtcgca cctcacccat caatgaagac
     1021 atccgctcca gcggccggaa ctcccccagc gctgccagta cctccagcaa tgacagtaaa
     1081 gcagagacag tgaagaagtc ggccaagaag gtgaaggagg aagcctcttc ccctcttaag
     1141 agtaacaaac gccagcggga gaaggtggcc tctgatacgg aggaggctga caggaccagc
     1201 tccaagaaga caaaaacgca ggagatcagc aggcccaact cgccatctga aggtgaggga
     1261 gagagttcag acagtcgcag cgtcaacgat gagggtagca gtgaccccaa agacatcgac
     1321 caggacaatc gcagcacgtc cccgagcatc cccagccccc aggacaatga gagtgactcg
     1381 gactcgtcag cccagcagca gatgctgcag gcccagcccc cagccttgca ggctcccact
     1441 ggggtcaccc cagctccctc ctcagctcct ccagggaccc ctcagctgcc cacgccaggg
     1501 cccacgccct ctgccactgc agttccccca cagggctccc ccacggcctc ccaggcccct
     1561 aaccagccac aggctcccac agcgcctgtt ccccacaccc acatccaaca ggcaccggcc
     1621 ttgcaccccc agcggccgcc ctcaccgcat cccccgccgc atccctcgcc acatcccccg
     1681 ctgcagcctc tgactgggtc ggcgggccag ccttctgcac cctctcatgc ccagccccca
     1741 ctgcacggtc agggcccacc cggccctcac agcctgcagg ctgggcccct gctgcagcac
     1801 ccaggccccc cacagccctt tggcctccct ccccaggcct cccaaggcca ggcccctctg
     1861 gggacctccc cagcagcagc gtaccctcac acctccctgc agctgccagc ctctcagtca
     1921 gcgctgcagt cccaacagcc tccacgggag cagcccctgc caccagcgcc cttggccatg
     1981 ccccacatca agcccccgcc taccactccc atcccccagc tgccggcgcc acaggcccac
     2041 aagcaccctc cccacctctc ggggccctca cccttctcca tgaatgccaa cctgcctccc
     2101 cctccagccc tgaagcccct gagctccctg tccacacatc accccccgtc ggctcacccc
     2161 ccacccctgc aactcatgcc tcagagccag ccattgccct cctcgcccgc ccagcccccc
     2221 gggctgaccc agagccagaa cctgcccccg ccccctgcct cccacccccc tacaggcctc
     2281 caccaggtgg ccccccaacc cccgtttgct cagcacccct ttgtccctgg aggccctcct
     2341 cccatcaccc ctccgacctg cccctccacc tctaccccac cggcgggacc tggcacctcg
     2401 gcccagccac cctgctctgg tgcggcggct tcaggaggca gcatagcggg ggggtcgtcc
     2461 tgcccactcc ccaccgtcca gatcaaggag gaggctctgg acgacgctga ggagcctgag
     2521 agcccccctc ccccaccaag gagcccgtcc ccggagccca ctgtggtgga cacccccagt
     2581 cacgccagcc agtcagctag gttctacaaa cacctggacc ggggctacaa ctcgtgtgcc
     2641 cggacagacc tgtacttcat gcctctggcc gggtccaagc tggccaagaa gagggaggag
     2701 gccattgaga aggccaagcg cgaggctgag cagaaagccc gagaggagcg agagcgggag
     2761 aaggagaagg agaaggagcg ggagcgggag cgagagcggg agcgcgaggc agagcgggcg
     2821 gctaaggcgt ccagctcagc gcatgaaggt cgcctcagtg acccacagct cagtggtcct
     2881 ggccacatgc ggccatcctt cgagccacca ccaaccacca ttgctgctgt gcccccctac
     2941 atcgggcccg acacacctgc ccttcggact ctgagcgagt acgcccggcc ccacgtcatg
     3001 tcgcccacca accgcaacca ccccttctac atgcccctta accccacgga ccccctgctg
     3061 gcctaccaca tgcctggcct ctacaacgtc gaccccacca tccgcgagcg ggagctccgg
     3121 gagcgggaga tccgagagcg ggagatccga gagcgggagc tgcgggagag gatgaagccg
     3181 ggcttcgagg tgaagccccc agagctggac cccctgcacc cagccgccaa ccccatggag
     3241 cactttgccc ggcacagcgc cctcaccatc cccccgaccg ccgggcccca cccttttgct
     3301 tctttccacc cgggcctgaa ccccttggag agggagagac tggccctggc gggcccccag
     3361 ctgcggcccg agatgagcta ccctgacaga ctggcagccg agcgtatcca cgcagagcgc
     3421 atggcatcgc tgaccagcga tcccctggcc cgactgcaga tgttcaacgt gactccgcac
     3481 catcaccagc actctcacat tcactcccac ctccacctcc accagcagga ccccctccac
     3541 caaggttcag caggccccgt tcacccgctg gtcgaccccc tgactgccgg tccccacctg
     3601 gctcgcttcc cctacccgcc tggcactctc cccaaccctc tgcttggaca gcccccacac
     3661 gagcacgaga tgcttcgcca cccagttttc ggcaccccct acccccgtga cctgcctggg
     3721 gccatcccac cccccatgtc agcagcccac cagctgcagg ccatgcatgc ccagtcggcc
     3781 gagctgcaga gactggccat ggagcagcag tggctgcatg gacaccccca catgcatggt
     3841 ggccacctac caagtcagga agattattac agtcgactga agaaagaagg tgacaagcag
     3901 ttatagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
     3961 tgtggaaagg acgatcaatc tcgtatgtac agcagata
//
