LOCUS       sequence-284382-        1712 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1188
FEATURES             Location/Qualifiers
     source          1..1712
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     RBS             562..573
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 accgccacca tggctgttgg gcttcttaaa gccatgtacc aggagttggt gaccttcaga
       61 gatgtggctg tagacttctc ccaagaggaa tgggattgtc tggattcttc tcaaagacat
      121 ctgtacagta atgtgatgct agagaactac aggatcttgg tatcactggg actttgcttt
      181 tccaaaccaa gtgtgatatt attgttggaa caaggaaaag caccctggat ggtgaagaga
      241 gagctgacaa aaggcttgtg ctcaggctgg gagcctatat gtgagactga agaattaacc
      301 ccaaagcagg atttttatga agaacatcaa tcccagaaga taatagaaac acttacaagc
      361 tataaccttg aatactccag tttgagagaa gagtggaaat gtgagggcta ttttgaaagg
      421 caaccaggta atcagaaggc gtgtttcaag gaagagataa tcactcatga agaacccctt
      481 tttgatgaga gagaacaaga atataaatct tggggaagtt ttcatcagaa cccactgctt
      541 tgtacacaaa agataatccc caaagaggag aaagtacata aacatgacac acaaaagaga
      601 agctttaaaa aaaatttaat ggctattaag cccaagagtg tctgtgcaga gaagaaactt
      661 ttgaaatgta atgactgtga aaaagtcttc agccagagtt catcccttac tcttcatcaa
      721 agaattcata ctggagagaa accctataaa tgtatagagt gtggaaaagc cttcagccag
      781 agatcaaatc ttgttcaaca tcagaggatt catactggag aaaaacccta tgaatgtaag
      841 gaatgtagga aagccttcag tcagaatgca cacctagttc aacatctgcg agttcatact
      901 ggagaaaaac cttacgaatg taaggtatgt cgaaaagcct tcagccagtt tgcctacctt
      961 gctcaacatc agagagttca cacgggagag aaaccctatg aatgtatcga atgtgggaaa
     1021 gcatttagca acagatcatc cattgctcaa caccagagag ttcatacagg agagaaaccc
     1081 tatgaatgta atgtctgtgg gaaagcattt agccttcgtg cataccttac tgtacatcag
     1141 agaatacata ctggagagag accctatgaa tgtaaggaat gtgggaaggc ctttagccag
     1201 aattcacacc ttgctcaaca tcagagaatt catactggag aaaaacctta taagtgtcag
     1261 gaatgtagga aagcattcag ccagattgcc taccttgctc agcatcaaag agttcatact
     1321 ggagagaaac cctatgaatg tattgaatgt gggaaggctt ttagcaatga ctcgtccctt
     1381 actcaacatc agcgagttca tactggagag aaaccttatg aatgtactgt ttgtggaaag
     1441 gcttttagtt actgtggatc ccttgcccaa catcagagaa ttcatactgg agagagaccc
     1501 tatgaatgta aggaatgcaa aaaaaccttc aggcagcatg cacaccttgc tcatcaccag
     1561 agaattcaca ttggggagtc actgtcacca cccaacccag tcaatcacca agtcctatag
     1621 taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
     1681 aaggacgagt acttcccggg cgtaaacctg tc
//
