LOCUS       sequence-284385-        1925 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1192
FEATURES             Location/Qualifiers
     source          1..1925
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggagtcttg ctctgtcgca caggctggag tgcagtggcc cgatctcagc
       61 tcactgcaac ctccgcctcc caggttcaag caattctcct gccacagcct ccaagtagct
      121 gggattacag atttggattt acagtgtgag ataatcagct acatagaagt acccacttat
      181 gaaacagata tatcctctac acaacttcag agcatatata agagagagaa actctatgaa
      241 tgtaagaaat gtcagaagaa atttagtagt ggttatcaac ttattctaca tcacaggttt
      301 catgtcattg agagacccta tgaatgcaaa gagtgtggga agaactttcg tagtggctat
      361 caacttactc tacatcaaag atttcatact ggtgagaaac cctatgaatg tacagaatgt
      421 gggaagaact ttagaagtgg ttatcagctg actgtgcatc agagatttca tactggtgag
      481 aaaacctatg aatgtaggca gtgtgggaag gcctttatat atgcctcaca cattgttcaa
      541 catgagagaa ttcacactgg tgggaagccg tatgaatgtc aggagtgtgg gagggccttt
      601 agtcaaggtg gacatcttag aattcatcag agagttcata ctggcgaaaa accatataaa
      661 tgtaaggaat gtgggaagac ttttagtagg cgctcaaatc ttgttgaaca tgggcagttt
      721 catactgatg agaagccata catatgtgag aaatgtggaa aggcctttag aagaggtcac
      781 cagcttactg tacatcagag agttcacact ggtaaaaagc catatgagtg taaagaatgt
      841 gggaagggct ataccactgc ctcatacttt cttctacatc agagaattca taaaggtgga
      901 aaaccctatg aatgtaagga gtgtaagaaa acctttactt tgtatagaaa tcttactcgg
      961 catcagaata ttcatactgg tgagaaactt tttgaatgca agcaatgtgg gaagacctat
     1021 actactggtt caaaactctt tcaacatcag aaaactcata ctggcgagaa accctatgaa
     1081 tgcaaggaat gcggaaaggc ctttagcttg tatggctacc ttaaacaaca tcagaaaatt
     1141 catactggca tgaaacactt tgaatgtaag gagtgtaaaa aaacctttac tttgtataga
     1201 aatcttactc gacatcagaa tattcacact ggtaagaaac tttttgaatg tcaggaatgt
     1261 gggaaggcct atagtactgg ctcaaacctt attcaacatc ggaaaactca tactggtgag
     1321 aaaccctata aatgtaagga atgtggcaag acctttagct tgcatggata tcttaatcaa
     1381 catcagaaaa ttcatactgg tatgaaaccc tatgaatgta aggtatgtag aaaaaccttt
     1441 actttctata gaaatcttac tctacatcaa agtattcata ctgatgagaa accttttgaa
     1501 tgtaaggaat gtgggaagac ctttagacgt agttcacacc ttactgcaca tcagagcatt
     1561 catgctgata aaaaacccta tgaatgtaaa gaatgtggaa aggcctttaa aatgtatggc
     1621 taccttaccc aacatcagaa aattcatact ggtggaaaac cttatgaatg taaagaatgt
     1681 gggaaggcct tcagtcgtgc ttcaaacctt gttcaacatg agagaattca tactggtgag
     1741 aaaccctatg tgtgtaagca gtgtgggaaa accttcagat atggttcagc ccttaaagcc
     1801 catcagagaa ttcataggag cataaaagtg tagtaatgaa ctagtccgta acttgaaagt
     1861 atttcgattt cttggcttta tatatcttgt ggaaaggacg accacattct tcgcacattg
     1921 ggtgc
//
