LOCUS       sequence-284391-        5381 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5381)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 5381)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1207
FEATURES             Location/Qualifiers
     source          1..5381
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3567..3578
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 accgccacca tggatcttgg aacagctgag ggcacccggt gcacggaccc gcctgcaggc
       61 aagcccgcca tggcgcccaa acgcaagggt ggcctgaagc tgaacgccat ctgcgccaag
      121 ctgagccgcc aggtggtggt ggagaagcga gctgacgccg gctcccacac ggagggcagc
      181 ccatcgcagc cccgggacca agagcgcagt ggccctgagt ctggggcagc ccgggccccc
      241 cgcagcgagg aagacaagag acgggcagtg atcgagaagt gggtgaacgg ggagtacagc
      301 gaggagccgg cacccacacc cgtgttgggg cggattgccc gcgagggcct ggagctgcct
      361 cccgagggtg tctacatggt gcagccccag gggtgcagcg atgaggaaga ccacgcggag
      421 gagccctcca aggacggcgg tgccctggag gagaaggatt cggacggggc agcctccaag
      481 gaggacagcg gccccagcac caggcaggct tcaggagagg cctcctcgct gcgggactac
      541 gcggcctcca ccatgaccga gttcctcggc atgtttggct atgatgacca gaacacgcgg
      601 gacgagctgg ccaggaagat cagctttgag aagctgcacg cgggctccac cccggaggca
      661 gccacctcct ccatgctgcc cacctccgag gataccctca gcaagcgggc gcggttctct
      721 aagtatgagg agtacatccg caagctcaag gctggcgagc agctctcctg gccggccccc
      781 agcaccaaga ccgaggagcg ggtgggcaag gaggtggtgg gcaccctgcc cggcctgcgg
      841 ctgcccagca gcacggccca cctggagacc aaggccacca tcctgcccct gccgtcgcac
      901 agcagtgtcc agatgcagaa cctggtagcc cgggcctcca agtacgactt cttcatccaa
      961 aaactgaaga ccggcgagaa tctgcggccc cagaacggga gcacctacaa gaagccatcc
     1021 aagtacgacc tggagaatgt caagtacctg cacctcttca aacccgggga gggcagcccc
     1081 gacatgggcg gggccatcgc cttcaagaca ggcaaggtgg ggcgcccttc caagtacgac
     1141 gtccggggca tccagaagcc aggccccgcc aaggttccgc ccacccccag cctggctccc
     1201 gcacccctcg ccagcgtgcc cagtgccccc agcgcccccg ggccagggcc agagcctcct
     1261 gcctccctgt ccttcaacac tcccgagtac ctgaagtcaa ccttctccaa aacagactcc
     1321 atcaccacgg ggaccgtctc cactgtcaag aacggactgc ccacagataa accagccgtc
     1381 actgaagatg taaacattta ccagaaatat attgccaggt tctcgggcag ccagcactgt
     1441 ggccacatcc actgtgccta ccagtaccgc gagcactacc actgccttga ccctgagtgt
     1501 aactaccaga ggttcacgag taagcaggac gtgatccgcc actacaacat gcacaagaag
     1561 cgcgacaact ccctgcagca cggcttcatg cgtttcagcc cgctggacga ctgcagcgtc
     1621 tactaccacg gctgccacct caatgggaag agcacccact atcactgcat gcaggtgggc
     1681 tgtaacaagg tgtacacgag cacgtctgac gtgatgaccc acgagaactt ccacaagaag
     1741 aatacccagc tcattaacga cggcttccag cgcttccgag ccaccgaaga ctgtggcaca
     1801 gccgactgcc agttctacgg acagaagacc acgcacttcc actgcaggcg ccccggctgc
     1861 acattcactt tcaagaacaa gtgtgacatc gagaagcaca agagctacca catcaaggac
     1921 gatgcctacg ccaaggacgg cttcaagaag ttctacaagt acgaggagtg caagtacgag
     1981 ggctgcgtgt acagcaaggc taccaaccac ttccactgca tccgcgccgg ctgcggcttc
     2041 accttcacct ccaccagcca gatgacctct cacaagcgca agcatgagcg ccggcacatc
     2101 cgctcctcgg gcgcgctggg gctgccgccc tcgctgctgg gcgccaagga cacggagcac
     2161 gaggagtcca gcaacgacga ccttgttgac ttctccgccc tgagcagcaa gaactccagc
     2221 ctgagcgcct cccctaccag ccagcagtcc tctgcgtccc tggctgccgc cactgccgcc
     2281 accgaggctg ggcccagtgc caccaaacct cccaacagca agatctcggg gctgctgccc
     2341 cagggcctgc ctggctcaat ccccctggcc ctggccctct ccaactcggg cctgcccacc
     2401 cccacgccct acttccccat actggctggc cgtgggagca cctccctgcc tgtgggcacc
     2461 cccagcctcc tgggtgccgt gtcgtctggg tcagcagcct cagccacccc tgacacaccc
     2521 acgctggtcg cctcgggagc tggagactca gcccccgtgg ctgccgcctc tgtcccggca
     2581 ccacccgcct ccatcatgga gaggatctct gcaagcaagg gcctcatctc gcccatgatg
     2641 gccaggctgg ctgcagctgc cctcaagccc tctgccacct ttgacccagg aagcgggcag
     2701 caggtcaccc cagccaggtt ccccccggcc caagtgaagc cggaacccgg tgagagcacc
     2761 ggcgccccag gcccccacga agcctcccag gaccgcagtc tagacctgac tgtgaaggag
     2821 cccagcaacg aatcaaatgg ccacgcagtc ccggcaaatt catctctttt atcctcgctt
     2881 atgaataaga tgtctcaggg caaccctggc ctgggcagcc tgctgaacat caaggcggaa
     2941 gcggagggga gccccgctgc ggagccctcg cccttcctag gcaaggccgt gaaggcgctg
     3001 gttcaggaga agttggcaga gccctggaag gtgtacctgc gcaggtttgg tacaaaggac
     3061 ttctgtgacg gccagtgtga cttcctccac aaggcccact tccactgcgt ggtggaggaa
     3121 tgcggcgcgc tcttcagcac cttggacggg gccatcaagc acgcaaactt ccacttccgg
     3181 acagagggag gagcagcaaa aggaaacaca gaggctgcct ttccggcctc ggccgccgag
     3241 accaaacctc ccatggcccc ctcgtcccct ccggtccctc ctgtcaccac ggccacggtg
     3301 tcctctctgg aggggcccgc tcccagcccg gcctccgtgc cctccacccc caccctgctc
     3361 gcctggaagc agctggcttc caccataccc cagatgcctc agatcccagc gtcagtgcct
     3421 cacctgcccg cctcgccctt ggcaacgact tctctagaga acgccaagcc ccaggtcaaa
     3481 cccggattcc tccagttcca ggagaacgat ccttgcctcg ccacggactg caagtacgcc
     3541 aacaagttcc acttccactg tctctttggg aactgcaagt acgtctgcaa aacgtctggc
     3601 aaggccgaat cccactgcct ggaccacatc aaccccaaca acaacctggt gaacgtgcga
     3661 gaccagtttg catactactc tctgcagtgt ctctgtccca accagcactg cgagttccga
     3721 atgcgtgggc actaccactg cctccgcacc ggctgctatt ttgtgaccaa catcaccacc
     3781 aagctcccct ggcacatcaa gaagcatgag aaggcggagc ggcgggcagc caatggcttc
     3841 aaatacttca ccaagcgcga ggagtgtggc aggctaggtt gcaagtacaa ccaggtgaac
     3901 agccacttcc actgcatccg ggagggctgc cagttctcct tcctcctcaa gcaccagatg
     3961 acctcccacg cgcggaagca catgcggagg atgctgggga agaacttcga ccgcgtgccc
     4021 ccctcccagg gccccccagg cctgatggat gctgagacag atgagtgcat ggactacact
     4081 ggctgcagcc caggcgccat gtcctctgag tcatccacca tggaccggag ctgctccagc
     4141 acccccgtgg gtaacgagag caccgcggca gggaacacca tctctatgcc gacagcctcg
     4201 ggggccaaaa agcgcttctg gatcatcgag gacatgtcgc ccttcggcaa gcggcggaag
     4261 acggcgtcct cccggaagat gctggacgag ggcatgatgc tggagggctt ccggcgcttc
     4321 gacctttacg aggactgcaa ggacgcagct tgtcagttct cgctcaaggt cacccactac
     4381 cactgcacgc gcgagaactg cggctacaag ttctgcgggc gcacgcacat gtacaagcac
     4441 gcgcagcacc acgaccgcgt ggacaacctg gtgctggacg acttcaagcg cttcaaggcc
     4501 tcactcagct gccacttcgc cgactgcccc ttctcgggca ccagcacgca cttccactgc
     4561 ctgcgctgcc gcttccgctg caccgacagc accaaggtca cggcgcatcg caagcaccac
     4621 ggcaaacagg acgtgatcag cgccgcgggc ttctgccagt tcagctccag cgccgactgc
     4681 gccgtgcccg actgcaagta caagctcaag tgctcgcact tccactgcac cttcccgggc
     4741 tgccgccaca cggtggtggg catgtcgcag atggactcgc acaagcgcaa gcacgagaag
     4801 caggagcgcg gcgagcccgc ggcagagggc cccgcgcccg ggcctcccat cagtctggac
     4861 ggctccctgt cgctgggcgc cgagccgggc tcgctgctct tcctgcagtc ggcggccgcc
     4921 ggcctgggcc tggcgctggg cgacgcgggc gaccccggcc cgcccgacgc cgccgccccc
     4981 gggccgcgcg agggcgccgc cgccgccgcc gccgcagctg gggagtcctc gcaggaggac
     5041 gaggaggagg agctggagct gccggaggag gaggccgaag acgacgagga cgaggacgac
     5101 gacgaggacg acgacgacga ggacgacgac gaggacgacg acgacgagga cctgcgcacc
     5161 gactcggagg agtcgctgcc cgaggcggcg gcggaggcgg cgggcgcggg cgcgcggacc
     5221 ccggccttgg cggcgctggc ggccctgggc gcccccggcc ccgcgcccac tgcagcctcc
     5281 tcgccctagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     5341 tcttgtggaa aggacgacac agtcaggctg cgagccatgt g
//
