LOCUS       sequence-284397-        2597 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1224
FEATURES             Location/Qualifiers
     source          1..2597
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggccagctc ggagactgag atccgctggg ctgagcctgg cctggggaag
       61 ggcccccagc ggcggcgctg ggcctgggcc gaggacaaga gggatgtgga tagaagtagt
      121 tcacaaagct gggaagaaga gagactcttt cccaatgcca ccagccccga gctcctagag
      181 gacttccgcc tggcccagca gcacctgccg cccctggagt gggacccaca cccgcagccc
      241 gatgggcatc aggattccga gtcaggagag acttcgggag aagaggctga agcagaggat
      301 gtggacagcc cagcaagttc ccatgagcct cttgcctggc tcccccagca gggccgtcag
      361 ctggacatga ctgaagagga gccagatggg accctcggaa gtctggaggt tgaggaggct
      421 ggagagagct cctcaaggtt ggggtatgag gctggtctca gcttggaagg ccatggaaac
      481 accagcccca tggctcttgg gcatggtcag gccaggggct gggtggcttc tggcgaacaa
      541 gccagtgggg acaaactttc tgaacattcc gaggtcaacc catccgttga actcagcccg
      601 gcaaggtcct ggagcagtgg gacagtgagc ctcgaccacc ctagtgacag ccttgattct
      661 acctgggaag gagagaccga tggcccccag cccactgccc tggcagaaac cttgccagag
      721 ggccccagcc accacctcct aagcccagat ggcagaactg gaggcagtgt tgctcgggca
      781 acccccatgg aattccagga ctcctcagct cccccagccc agagtccgca gcatgccaca
      841 gatagatgga ggagagaaac gaccagattc ttctgccctc agcccaagga acacatctgg
      901 aagcagacaa agacgtcacc taagccactc ccttcccgat tcattggctc catcagcccc
      961 ctgaatcccc agcccaggcc aacgcggcag ggcaggccgc tgcccagaca gggagccact
     1021 ctggctggcc gctcctcttc taatgccccc aagtatggcc gggggcagtt gaactaccca
     1081 ctccctgatt tctccaaggt agggccccgg gtgagattcc ccaaagatga gagctaccgt
     1141 ccccccaagt ccagaagcca caacaggaag cctcaggccc ctgccaggcc cctcatcttc
     1201 aagtctccag ctgagattgt gcaggaggtg ctgttgagca gtggagaagc agccctggca
     1261 aaggacacgc ctcctgccca ccctatcacc agggtacccc aagaatttca gacgcctgag
     1321 caagccactg agctggtcca tcagctccag gaagactacc acaggctcct caccaagtac
     1381 gctgaggccg agaacaccat tgaccagcta cgcctcgggg ccaaggtgaa cctgttctct
     1441 gacccacccc agcccaacca cagcatccac acgggaatgg tgccccaggg gaccaaggtc
     1501 ttgtccttca ccatcccaca gccccgctct gcagagtggt ggccgggccc ggccgaggac
     1561 ccccaggcct ctgcggcctc agggtggcca tcagctcgag gagacttgag cccctcctcg
     1621 cttaccagca tgcccaccct ggggtggctt ccggagaacc gggacatctc tgaggaccag
     1681 tcctcagcag agcagaccca ggcactggct tctcaggcca gccagttcct ggccaaggtg
     1741 gagtcctttg aaagactgat acaggcagga cgtctcatgc cccaggacca agtcaagggc
     1801 ttccagcggc tgaaggctgc ccacgcggcc ctagaggagg agtacctgaa ggcttgtcgg
     1861 gagcaacacc ctgcccagcc gcttgccggc tccaagggga cgcctggaag atttgatcct
     1921 cgcagggagc tggaggcaga gatataccgt ctgggaagct gcctggaaga gctgaaggaa
     1981 cacatagacc agacccagca agagcctgag ccgcccgggt cagactcagc tctggacagc
     2041 accccagccc tgccctgcct ccatcagcca acgcacctgc ctgctccttc tggacaagcc
     2101 cccatgccag ccatcaagac ctcctgccct gagcctgcta ccaccactgc cgccgccagc
     2161 actggcccct gcccattgca cgtaaatgtg gaggtgagct ctggcaacag tgaggtggag
     2221 gacaggccac aggaccccct ggcccgactc aggcacaagg agctgcagat ggagcaagtt
     2281 taccatggcc tcatggagcg gtacctcagt gtgaagtctc tcccagaagc catgagaatg
     2341 gaggaggagg aagaaggaga ggaggaggag gaggaagagg ggggaggtga ctccctggaa
     2401 gttgatgggg tggctgcaac tccagggaaa gcagaggcca ccagggtcct cccaaggcag
     2461 tgcccggtgc aggctgagaa aagtcatggg gctcccctgg agtagtaatg aactagtccg
     2521 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgaccgataa
     2581 taattcgata taagcac
//
