LOCUS       sequence-284400-        3857 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3857)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1230
FEATURES             Location/Qualifiers
     source          1..3857
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(362..373)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     regulatory      686..695
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggacagctt cgacttagcc ctgctccagg aatgggacct cgagtcactg
       61 tggggtgaag acatcttaaa ccagaggaat gactctctag ttgtggaatt tcagtcatca
      121 gccagcagat gcaggagtgt ctatgaacca gatagaaatg cattacggag gaaagaacga
      181 gaaagaagaa atcaagaaac tcaacaggat gatggcacgt ttaattctag ttactctctc
      241 ttcagtgagc cctacaagac taacaagggg gatgaactct ccaaccggat ccagaacact
      301 ttaggcaatt atgatgaaat gaaagacttt ttaactgata gatccaatca gagtcatctc
      361 gttggagttc ccaaacctgg ggttcctcag actcctgtga acaagatcga tgaacatttt
      421 gttgcagatt caagagccca gaaccagccc tcgtctatct gtagcactac aacttccaca
      481 ccagcagctg tccccgtgca gcagagtaag agaggcacta tgggctggca gaaggctggg
      541 cacccaccct ctgacggcca acagagagca acacaacagg gctctctcag gaccttgctt
      601 ggagatggtg ttggcagaca gcagcctcgg gccaaacaag tgtgcaatgt ggaggtgggc
      661 cttcagaccc aggagaggcc acctgccatg gcggccaagc acagcagcag cggacactgt
      721 gttcagaact ttcctccatc cctagcttca aaacccagcc tggtccagca gaaaccgacc
      781 gcgtatgtga ggccaatgga cggccaagat caggcccctg atgagtctcc taagctgaag
      841 tcgtcttcgg aaaccagcgt gcactgcaca tcatacaggg gagtccctgc cagcaagccg
      901 gagcctgcca gagccaaggc caagctctcc aagttcagca tccccaagca gggggaggag
      961 agtagatctg gagaaaccaa cagctgtgtt gaagaaataa tccgggagat gacctggctt
     1021 ccaccacttt ctgctattca agcacctggc aaagtggaac caaccaaatt tccatttcca
     1081 aataaggact ctcagcttgt atcctctgga cacaataatc caaagaaagg tgatgcagag
     1141 ccagagagtc cagacaatgg cacatcgaat acatcaatgc tggaagatga ccttaagcta
     1201 agcagtgatg aagaggagaa tgaacagcag gcagctcaga gaacggctct ccgcgctctc
     1261 tctgacagcg ccgtggtcca gcagcccaac tgcagaacct cggtgccttc cagcaagggc
     1321 agcagcagca gcagcagcag cggcagcagc agctcctcca gcgactcaga gagcagctcc
     1381 ggatctgact cggagaccga gagcagctcc agcgagagtg agggcagcaa gcccccccac
     1441 ttctccagcc ccgaggctga accggcatcc tctaacaagt ggcagctgga taaatggcta
     1501 aacaaagtta atccccacaa gcctcctatt ctgatccaaa atgaaagcca cgggtcagag
     1561 agcaatcagt actacaaccc ggtgaaagag gacgtccagg actgtgggaa agtccccgac
     1621 gtttgccagc ccagcctgag agagaaggag atcaagagca cttgcaagga ggagcaaagg
     1681 ccaaggacag ccaacaaggc ccctgggagt aaaggcgtga agcagaagtc cccgcccgcg
     1741 gccgtggccg tggcggtgag cgcagccgcc ccgccacccg cagtgccctg tgcgcccgcg
     1801 gagaacgcgc ccgcgcctgc ccggaggtcc gcgggcaaga agcccaccag gcgcaccgag
     1861 aggacctcag ccggggacgg cgccaactgc caccggcccg aggagcccgc ggccgcggac
     1921 gcgctgggga cgagcgtggt ggtccccccg gagcccacca aaaccaggcc ctgtggcaac
     1981 aacagagcga gccaccgcaa ggagctgcgc tcctccgtga cctgcgagaa gcgccgcacg
     2041 cgggggctaa gcaggatcgt ccccaaatcc aaggagttca ttgagacaga gtcgtcatct
     2101 tcatcctcct cctcggactc cgacctggag tccgagcagg aggagtaccc tctgtccaaa
     2161 gcacagaccg tggctgcctc tgcctcctcc gggaatgatc agaggctgaa ggaggccgct
     2221 gccaacgggg gcagtggtcc tagggcccct gtaggctcca tcaacgccag gaccaccagt
     2281 gacatcgcca aggagctgga ggagcagttc tacacactgg tcccctttgg ccggaacgaa
     2341 cttctctccc ctctaaagga cagtgatgag atcaggtctc tctgggtcaa aatcgacctg
     2401 accctcctgt ccaggatccc agaacacctg ccccaggagc caggggtatt gagcgcccct
     2461 gccaccaagg actctgagag cgcaccgccc agccacacct cggacacacc tgcagaaaag
     2521 gctttgccaa aatccaagag gaaacgcaag tgtgacaacg aagacgacta cagggagatc
     2581 aagaagtccc agggagagaa agacagctct tcaagactgg ccacctccac cagtaatact
     2641 ttgtctgcaa accactgcaa catgaacatc aacagtgtgg caataccaat aaataaaaat
     2701 gaaaaaatgc ttcggtcgcc catctcaccc ctctctgatg catctaaaca caaatacacc
     2761 agcgaggact taacttcttc cagccgacct aatggcaaca gtttgtttac ttcagcctct
     2821 tccagcaaaa agcctaaggc cgacagccag ctgcagcctc acggcggaga cctcacgaaa
     2881 gcagctcaca acaattctga aaacattccc ctccacaagt cacggccgca gacgaagccg
     2941 tggtctccag gctccaacgg ccacagggac tgcaagaggc agaaacttgt cttcgatgat
     3001 atgcctcgca gtgccgatta ttttatgcaa gaagctaaac gaatgaagca taaagcagat
     3061 gcaatggtgg aaaagtttgg aaaggctttg aactatgctg aagcagcatt gtcgtttatc
     3121 gagtgtggaa atgcaatgga acaaggcccc atggaatcca aatctcctta tacgatgtat
     3181 tcagaaacag tagagctcat caggtatgct atgagactaa aaacccactc aggccccaat
     3241 gccacaccag aagacaaaca actggctgca ttatgttacc gatgcctggc cctcctgtac
     3301 tggcggatgt ttcgactcaa aagggaccac gctgtaaagt attcaaaagc actaatcgac
     3361 tatttcaaga actcatctaa agccgcccaa gccccatctc cgtggggggc cagtggaaag
     3421 agcactggaa ccccatcccc catgtctccc aacccctctc ccgccagctc cgtggggtct
     3481 cagggcagcc tctccaacgc cagcgccctg tccccgtcga ccatcgtcag catcccacag
     3541 cgcatccacc agatggcggc caaccacgtc agcatcacca acagcatcct gcacagctac
     3601 gactactggg agatggccga caacctggcc aaggaaaacc gagaattctt caacgacctg
     3661 gatctgctca tggggccggt caccctgcac agcagcatgg agcacctggt ccagtactcc
     3721 caacagggcc tgcactggct gcggaacagc gcccacctgt catagtaatg aactagtccg
     3781 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgatggttcg
     3841 agtcaggcta gagttca
//
