LOCUS       sequence-284403-        3932 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3932)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1232
FEATURES             Location/Qualifiers
     source          1..3932
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggatctatt cgactttttc agagactggg acttggagca gcagtgtcac
       61 tatgaacaag accgtagtgc acttaaaaaa agggaatggg agcggaggaa tcaagaagtc
      121 cagcaagaag acgatctctt ttcttcaggc tttgatcttt ttggggagcc atacaaggta
      181 gctgaatata caaacaaagg tgatgcactt gccaaccgag tccagaacac gcttggaaac
      241 tatgatgaaa tgaagaattt gctaactaac cattctaatc agaatcacct agtgggaatt
      301 ccaaagaatt ctgtgcccca gaatcccaac aacaaaaatg aaccaagctt ttttccagaa
      361 caaaagaaca gaataattcc acctcaccag gataataccc atccttcagc accaatgcct
      421 ccaccttctg ttgtgatact gaattcaact ctaatacaca gcaacagaaa atcaaaacct
      481 gagtggtcac gtgatagtca taaccctagc actgtactgg caagccaggc cagtggtcag
      541 ccaaacaaga tgcagacttt gacacaggac cagtctcaag ccaaactgga agacttcttt
      601 gtctacccag ctgaacagcc ccagattgga gaagttgaag agtcaaaccc atctgcaaag
      661 gaagacagta accctaattc tagtggagaa gatgctttca aagaaatctt tcaatccaat
      721 tcaccggaag aatctgaatt cgccgtgcaa gcgcctgggt ctcccctagt ggcttcctct
      781 ttattagctc ctagcagtgg cctttcagtt caaaacttcc caccagggct ttactgcaaa
      841 acaagcatgg ggcagcaaaa gccaactgca tacgtcagac ccatggatgg ccaggaccag
      901 gcaccggaca tctcaccaac actgaaacct tcaattgaat ttgagaacag ctttgggaat
      961 ctgtcatttg gaacactctt ggatggaaaa cccagtgcag ccagttcaaa gactaaactg
     1021 ccaaagttca ccatcctcca aacaagtgaa gtaagccttc ccagtgatcc aagctgtgtt
     1081 gaagaaatct tgcgggaatc gcagcatctg accccaggat tcaccttaca aaagtggaat
     1141 gacccaacca ccagagcttc tacaaagatg cttgaggatg acctgaagct gagcagtgat
     1201 gaagatgacc ttgagcctgt gaagaccttg accactcagt gcactgccac tgagctctac
     1261 caggctgttg aaaaggcaaa acctaggaat aatcctgtga acccaccctt ggccactccc
     1321 cagcccccac ctgcagtgca agccagcggg ggttctggca gctccagcga atcggagagc
     1381 agctctgagt cggattcaga cactgaaagt agcaccactg acagcgaatc taatgaggca
     1441 cctcgtgtgg caactccaga gcctgagcca ccctcaacca acaagtggca actggataaa
     1501 tggcttaaca aagtgacatc ccagaacaag tcttttattt gtggccaaaa tgaaacaccc
     1561 atggagacta tttctctgcc tcctccaatc atccaaccaa tggaagtcca gatgaaagtg
     1621 aagacgaatg ccagtcaggt cccagctgaa cccaaagaaa ggcctctcct cagtctcatt
     1681 agggagaaag cccgtccacg gcccactcag aaaattccag aaacaaaggc tttgaagcat
     1741 aagttgtcaa caactagtga gacagtgtct caaaggacaa ttgggaaaaa acagcccaaa
     1801 aaagttgaga agaacaccag cactgacgag tttacctggc ccaaaccaaa tattaccagc
     1861 agcactccca aagaaaaaga aagtgtggag cttcatgacc caccaagagg ccgcaacaaa
     1921 gccactgccc acaaaccagc ccctaggaaa gaaccaagac ctaacatccc tttggctccc
     1981 gagaagaaga agtacagagg gcctggcaag attgtgccaa agtctcggga attcattgaa
     2041 acagattcat ctacatctga ctccaacaca gatcaggaag agaccctgca aatcaaagtc
     2101 ctgcctccgt gcattatttc tggaggtaat actgccaaat ccaaggaaat ctgtggtgcc
     2161 agcctgaccc tcagcacctt aatgagtagc agtggcagca acaacaactt atccatcagt
     2221 aatgaagagc caacattttc acctattcct gtcatgcaaa ctgaaatcct gtcccctctg
     2281 cgagatcatg agaacctgaa aaacctctgg gtgaagattg accttgactt actctctaga
     2341 gtacctggcc acagctcact ccatgcagca cctgccaagc cagaccacaa ggagactgcc
     2401 acaaaaccca agcgtcagac agctgtcaca gctgtggaga aaccagcccc taagggcaaa
     2461 cgtaagcaca agccaataga agttgcagag aagatccctg agaagaagca gcgcctggag
     2521 gaggccacaa ctatctgctt gctccctcct tgcatctcac cagccccacc ccacaagcct
     2581 cccaacacta gagaaaataa ttcatccagg agagcaaata gaagaaagga agaaaaacta
     2641 tttcctcctc cactttcccc actgccagag gaccctccac gccgcagaaa tgtcagtggc
     2701 aataatggtc cctttggtca agacaaaaac atcgccatga ctggacaaat cacatctacc
     2761 aaacctaaga gaactgaagg caaattctgt gctactttca aagggatatc ggtaaatgag
     2821 ggagacactc caaaaaaggc atcctctgcc accatcactg tcaccaatac tgctattgcc
     2881 actgctactg tcactgctac tgccattgtc accaccactg tcacagctac tgccaccgcc
     2941 acggccacca ccacaactac taccactacc atttccacca tcacctctac catcactact
     3001 ggcctcatgg atagcagtca cctggagatg acgtcctggg cggctctgcc ccttctatcc
     3061 agcagcagca ctaatgtccg gagacccaag ctcacttttg atgactcggt tcacaatgct
     3121 gattattaca tgcaagaagc taagaagctg aagcacaaag ctgatgcact gttcgagaaa
     3181 tttggcaaag ctgtgaatta tgctgatgcc gccctctcct tcactgaatg tggcaatgcc
     3241 atggaacgcg accctctgga agcaaagtcc ccatacacca tgtactctga gactgtggag
     3301 ctcctcaggt atgcaatgag gctgaagaac tttgcaagtc ccttggcttc ggatggggac
     3361 aaaaagctag cagtactatg ctaccgatgt ttatcactcc tctatttgag aatgtttaag
     3421 ctgaagaagg accatgctat gaagtactcc agatcactga tggaatattt taagcaaaat
     3481 gcttcaaaag tcgcacagat accctctcca tgggtaagca atggaaagaa cactccatcc
     3541 ccagtgtctc tcaacaacgt ctcccccatc aacgcaatgg ggaactgtaa caatggccca
     3601 gtcaccattc cccagcgcat tcaccacatg gctgccagcc acgtcaacat cactagcaat
     3661 gtgttacggg gctatgaaca ctgggatatg gccgacaaac tgacaagaga aaacaaagaa
     3721 ttctttggtg atctggacac gctgatgggg cctctgaccc agcacagcag catgaccaat
     3781 cttgtccgct acgttcgcca aggactgtgt tggctgcgca tcgatgccca cttgttgtag
     3841 taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
     3901 aaggacgaac cacagtacca aaccggatac ta
//
