LOCUS       sequence-284415-        2066 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1247
FEATURES             Location/Qualifiers
     source          1..2066
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcattcag ggatgtggct gtggatttca cccaggatga gtggaggctg
       61 ctgagccctg ctcaaaggac tctgtacaga gaggtgatgc tggagaacta cagcaacctg
      121 gtctcactgg gaatttcatt ttctaaacca gaactcatca cccagctgga gcaagggaaa
      181 gagacctgga gagaggaaaa aaaatgttca ccggcaacct gtccagcaga tccagagcca
      241 gagctctacc tcgatccttt ctgccctccg ggtttctcca gtcagaaatt ccccatgcag
      301 catgtgctgt gtaatcatcc cccctggatc ttcacatgct tgtgtgcaga aggtaacatc
      361 cagcctgggg atccgggccc aggggaccag gagaagcagc aacaagcctc tgaggggaga
      421 ccctggagtg atcaagcaga aggtcctgag ggagaaggtg ccatgccttt gtttggaaga
      481 accaagaaaa ggactctggg agcgttctcc aggccacccc agaggcagcc agtcagctct
      541 cggaacggcc tcagaggggt ggagttagaa gccagcccag ctcagtcagg gaaccctgag
      601 gaaacagaca aattgttgaa gaggatagaa gtcttaggat ttggaacagt caactgtgga
      661 gagtgtggac tgagcttcag caagatgaca aacctgctca gtcaccagcg gatacactca
      721 ggggagaagc cctacgtgtg tggggtatgt gagaagggct tcagcctaaa gaagagcctc
      781 gccagacacc agaaggcaca ctcgggggag aagccaattg tgtgcaggga gtgtggacga
      841 ggctttaacc ggaagtcaac gctaatcata cacgaacgga cacactccgg tgagaaacct
      901 tacatgtgca gtgagtgtgg gcgaggcttc agccagaagt caaacctcat catacaccag
      961 aggacacact caggggaaaa gccttacgtg tgccgggaat gtggcaaagg cttcagccag
     1021 aagtcagctg tcgtgagaca ccagaggaca cacttggagg agaagaccat cgtgtgcagt
     1081 gactgtggcc tgggcttcag cgacaggtca aacctcatct cccaccagag gacgcactct
     1141 ggggagaagc cctacgcctg caaggagtgt gggcgatgct tcaggcagag gaccaccctt
     1201 gtcaaccacc agaggacaca ctcaaaggag aagccctatg tgtgcggggt gtgtgggcac
     1261 agcttcagcc agaattcaac cctcatctct cacaggcgga cacacactgg ggagaagccg
     1321 tatgtttgtg gggtgtgtgg gcgaggcttt agtctcaagt cacacctcaa cagacaccag
     1381 aacatacact caggagagaa gcccattgtg tgcaaggact gtggccgggg cttcagccag
     1441 caatccaacc tcatcagaca ccagaggacg cactcaggcg agaagcccat ggtgtgtggg
     1501 gagtgcgggc gaggcttcag ccagaagtca aaccttgttg cacaccagag gacgcactca
     1561 ggggagaggc cgtatgtgtg ccgagagtgc gggcgaggct ttagccacca ggccggtctc
     1621 atcaggcaca agcggaagca ctcgagggag aagccctaca tgtgcaggca gtgtggactg
     1681 ggctttggca ataagtcagc tctaattaca cacaagcggg ctcactcgga agagaagcct
     1741 tgtgtgtgca gagagtgtgg ccaaggcttt ctccaaaagt cacacctcac cttacatcaa
     1801 atgacacata cgggggagaa gccatatgtg tgcaagacgt gtgggcgggg cttcagcctc
     1861 aagtctcacc tcagcagaca caggaagacc acgtctgtcc accacagact gccagtgcag
     1921 cccgaccctg agccgtgtgc agggcaacct tcggattcct tatactctct ctagtaatga
     1981 actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
     2041 gaggactgaa ggttataagg ctagcc
//
