LOCUS       sequence-284418-        2009 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1248
FEATURES             Location/Qualifiers
     source          1..2009
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggagctgc cggccttcag ggcgcgcctg gtggcgccga gcatcctggg
       61 aggtgtggtc cgggcgctgt gccgaggatt cggggtagtg tagtcctggc gccccgctgg
      121 aggagctccc cagctggtgg ctggagcgac cccttgttct ttggtggcgc tgatccagag
      181 ccagagctct acctcgatcc tttctgccct ccgggtttct ccagtcagaa attccccatg
      241 cagcatgtgc tgtgtaatca tcccccctgg atcttcacat gcttgtgtgc agaaggtaac
      301 atccagcctg gggatccggg cccaggggac caggagaagc agcaacaagc ctctgagggg
      361 agaccctgga gtgatcaagc agaaggtcct gagggagaag gtgccatgcc tttgtttgga
      421 agaaccaaga aaaggactct gggagcgttc tccaggccac cccagaggca gccagtcagc
      481 tctcggaacg gcctcagagg ggtggagtta gaagccagcc cagctcagtc agggaaccct
      541 gaggaaacag acaaattgtt gaagaggata gaagtcttag gatttggaac agtcaactgt
      601 ggagagtgtg gactgagctt cagcaagatg acaaacctgc tcagtcacca gcggatacac
      661 tcaggggaga agccctacgt gtgtggggta tgtgagaagg gcttcagcct aaagaagagc
      721 ctcgccagac accagaaggc acactcgggg gagaagccaa ttgtgtgcag ggagtgtgga
      781 cgaggcttta accggaagtc aacgctaatc atacacgaac ggacacactc cggtgagaaa
      841 ccttacatgt gcagtgagtg tgggcgaggc ttcagccaga agtcaaacct catcatacac
      901 cagaggacac actcagggga aaagccttac gtgtgccggg aatgtggcaa aggcttcagc
      961 cagaagtcag ctgtcgtgag acaccagagg acacacttgg aggagaagac catcgtgtgc
     1021 agtgactgtg gcctgggctt cagcgacagg tcaaacctca tctcccacca gaggacgcac
     1081 tctggggaga agccctacgc ctgcaaggag tgtgggcgat gcttcaggca gaggaccacc
     1141 cttgtcaacc accagaggac acactcaaag gagaagccct atgtgtgcgg ggtgtgtggg
     1201 cacagcttca gccagaattc aaccctcatc tctcacaggc ggacacacac tggggagaag
     1261 ccgtatgttt gtggggtgtg tgggcgaggc tttagtctca agtcacacct caacagacac
     1321 cagaacatac actcaggaga gaagcccatt gtgtgcaagg actgtggccg gggcttcagc
     1381 cagcaatcca acctcatcag acaccagagg acgcactcag gcgagaagcc catggtgtgt
     1441 ggggagtgcg ggcgaggctt cagccagaag tcaaaccttg ttgcacacca gaggacgcac
     1501 tcaggggaga ggccgtatgt gtgccgagag tgcgggcgag gctttagcca ccaggccggt
     1561 ctcatcaggc acaagcggaa gcactcgagg gagaagccct acatgtgcag gcagtgtgga
     1621 ctgggctttg gcaataagtc agctctaatt acacacaagc gggctcactc ggaagagaag
     1681 ccttgtgtgt gcagagagtg tggccaaggc tttctccaaa agtcacacct caccttacat
     1741 caaatgacac atacggggga gaagccatat gtgtgcaaga cgtgtgggcg gggcttcagc
     1801 ctcaagtctc acctcagcag acacaggaag accacgtctg tccaccacag actgccagtg
     1861 cagcccgacc ctgagccgtg tgcagggcaa ccttcggatt ccttatactc tctctagtaa
     1921 tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
     1981 gacgaccgaa tgtctaatgc agtggcggt
//
