LOCUS       sequence-284421-        2075 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1249
FEATURES             Location/Qualifiers
     source          1..2075
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcacacat ggcattcagg gatgtggctg tggatttcac ccaggatgag
       61 tggaggctgc tgagccctgc tcaaaggact ctgtacagag aggtgatgct ggagaactac
      121 agcaacctgg tctcactggg aatttcattt tctaaaccag aactcatcac ccagctggag
      181 caagggaaag agacctggag agaggaaaaa aaatgttcac cggcaacctg tccagcagat
      241 ccagagccag agctctacct cgatcctttc tgccctccgg gtttctccag tcagaaattc
      301 cccatgcagc atgtgctgtg taatcatccc ccctggatct tcacatgctt gtgtgcagaa
      361 ggtaacatcc agcctgggga tccgggccca ggggaccagg agaagcagca acaagcctct
      421 gaggggagac cctggagtga tcaagcagaa ggtcctgagg gagaaggtgc catgcctttg
      481 tttggaagaa ccaagaaaag gactctggga gcgttctcca ggccacccca gaggcagcca
      541 gtcagctctc ggaacggcct cagaggggtg gagttagaag ccagcccagc tcagtcaggg
      601 aaccctgagg aaacagacaa attgttgaag aggatagaag tcttaggatt tggaacagtc
      661 aactgtggag agtgtggact gagcttcagc aagatgacaa acctgctcag tcaccagcgg
      721 atacactcag gggagaagcc ctacgtgtgt ggggtatgtg agaagggctt cagcctaaag
      781 aagagcctcg ccagacacca gaaggcacac tcgggggaga agccaattgt gtgcagggag
      841 tgtggacgag gctttaaccg gaagtcaacg ctaatcatac acgaacggac acactccggt
      901 gagaaacctt acatgtgcag tgagtgtggg cgaggcttca gccagaagtc aaacctcatc
      961 atacaccaga ggacacactc aggggaaaag ccttacgtgt gccgggaatg tggcaaaggc
     1021 ttcagccaga agtcagctgt cgtgagacac cagaggacac acttggagga gaagaccatc
     1081 gtgtgcagtg actgtggcct gggcttcagc gacaggtcaa acctcatctc ccaccagagg
     1141 acgcactctg gggagaagcc ctacgcctgc aaggagtgtg ggcgatgctt caggcagagg
     1201 accacccttg tcaaccacca gaggacacac tcaaaggaga agccctatgt gtgcggggtg
     1261 tgtgggcaca gcttcagcca gaattcaacc ctcatctctc acaggcggac acacactggg
     1321 gagaagccgt atgtttgtgg ggtgtgtggg cgaggcttta gtctcaagtc acacctcaac
     1381 agacaccaga acatacactc aggagagaag cccattgtgt gcaaggactg tggccggggc
     1441 ttcagccagc aatccaacct catcagacac cagaggacgc actcaggcga gaagcccatg
     1501 gtgtgtgggg agtgcgggcg aggcttcagc cagaagtcaa accttgttgc acaccagagg
     1561 acgcactcag gggagaggcc gtatgtgtgc cgagagtgcg ggcgaggctt tagccaccag
     1621 gccggtctca tcaggcacaa gcggaagcac tcgagggaga agccctacat gtgcaggcag
     1681 tgtggactgg gctttggcaa taagtcagct ctaattacac acaagcgggc tcactcggaa
     1741 gagaagcctt gtgtgtgcag agagtgtggc caaggctttc tccaaaagtc acacctcacc
     1801 ttacatcaaa tgacacatac gggggagaag ccatatgtgt gcaagacgtg tgggcggggc
     1861 ttcagcctca agtctcacct cagcagacac aggaagacca cgtctgtcca ccacagactg
     1921 ccagtgcagc ccgaccctga gccgtgtgca gggcaacctt cggattcctt atactctctc
     1981 tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     2041 ggaaaggacg agttatagca taaggctcac tttgg
//
