LOCUS       sequence-284436-        2543 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1464
FEATURES             Location/Qualifiers
     source          1..2543
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggagctgtg gcgccaatgc acccactggc tcatccagtg ccgggtgctg
       61 ccgcccagcc accgcgtgac ctgggatggg gctcaggtgt gtgaactggc ccaggccctc
      121 cgggatggtg tccttctgtg tcagctgctt aacaacctgc taccccatgc catcaacctg
      181 cgtgaggtca acctgcgccc ccagatgtcc cagttcctgt gccttaagaa cattagaacc
      241 ttcctgtcca cctgctgtga gaagttcggc ctcaagcgga gcgagctctt cgaagccttt
      301 gacctcttcg atgtgcagga ttttggcaag gtcatctaca ccctgtctgc tctgtcctgg
      361 accccgatcg cccagaacag ggggatcatg cccttcccca ccgaggagga gagtgtaggt
      421 gatgaagaca tctacagtgg cctgtccgac cagatcgacg acacggtgga ggaggatgag
      481 gacctgtatg actgcgtgga gaatgaggag gcggaaggcg acgagatcta tgaggacctc
      541 atgcgctcgg agcccgtgtc catgccgcat ttcttgaagc ccctgcaacg gttcctgaaa
      601 cctcaagaca ttgagatcat ctttatcaac attgaggacc tgcttcgtgt tcatactcac
      661 ttcctaaagg agatgaagga agccctgggc acccctggcg cagccaatct ctaccaggtc
      721 ttcatcaaat acaaggagag gttcctcgtc tatggccgct actgcagcca ggtggagtca
      781 gccagcaaac acctggaccg tgtggccgca gcccgggagg acgtgcagat gaagctggag
      841 gaatgttctc agagagccaa caacgggagg ttcaccctgc gggacctgct gatggtgcct
      901 atgcagcgag ttctcaaata tcacctcctt ctccaggagc tggtgaaaca cacgcaggag
      961 gcgatggaga aggagaacct gcggctggcc ctggatgcca tgagggacct ggctcagtgc
     1021 gtgaacgagg tcaagcgaga caacgagaca ctgcgacaga tcaccaattt ccagctgtcc
     1081 attgagaacc tggaccagtc tctggctcac tatggccggc ccaagatcga cggggaactc
     1141 aagatcacct cggtggaacg gcgctccaag atggacaggt atgccttcct gctcgacaaa
     1201 gctctactca tctgtaagcg caggggagac tcctatgacc tcaaggactt tgtaaacctg
     1261 cacagcttcc aggttcggga tgactcttca ggagaccgag acaacaagaa gtggagccac
     1321 atgttcctcc tgatcgagga ccaaggtgcc cagggctatg agctgttctt caagacaaga
     1381 gaattgaaga agaagtggat ggagcagttt gagatggcca tctccaacat ctatccggag
     1441 aatgccaccg ccaacgggca tgacttccag atgttctcct ttgaggagac cacatcctgc
     1501 aaggcctgtc agatgctgct tagaggtacc ttctatcagg gctaccgctg ccatcggtgc
     1561 cgggcatctg cacacaagga gtgtctgggg agggtccctc catgtggccg acatgggcaa
     1621 gatttcccag gaactatgaa gaaggacaaa ctacatcgca gggctcagga caaaaagagg
     1681 aatgagctgg gtctgcccaa gatggaggtg tttcaggaat actacgggct tcctccaccc
     1741 cctggagcca ttggaccctt tctacggctc aaccctggag acattgtgga gctcacgaag
     1801 gctgaggctg aacagaactg gtgggagggc agaaatacat ctactaatga aattggctgg
     1861 tttccttgta acagggtgaa gccctatgtc catggccctc ctcaggacct gtctgttcat
     1921 ctctggtacg caggccccat ggagcgggca ggggcagaga gcatcctggc caaccgctcg
     1981 gacgggactt tcttggtgcg gcagagggtg aaggatgcag cagaatttgc catcagcatt
     2041 aaatataacg tcgaggtcaa gcacattaaa atcatgacag cagaaggact gtaccggatc
     2101 acagagaaaa aggctttccg ggggcttacg gagctggtgg agttttacca gcagaactct
     2161 ctaaaggatt gcttcaagtc tctggacacc accttgcagt tccccttcaa ggagcctgaa
     2221 aagagaacca tcagcaggcc agcagtggga agcacaaagt attttggcac agccaaagcc
     2281 cgctatgact tctgcgcccg agaccgatca gagctgtcgc tcaaggaggg tgacatcatc
     2341 aagatcctta acaagaaggg acagcaaggc tggtggcgag gggagatcta tggccgggtt
     2401 ggctggttcc ctgccaacta cgtggaggaa gattattctg aatactgcta gtaatgaact
     2461 agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgac
     2521 gctactacgc gcacttggac tca
//
