LOCUS       sequence-284454-        2099 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1753
FEATURES             Location/Qualifiers
     source          1..2099
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaagagca ggatgagaag cccccagagc ccccgaaggt ctgtgcacag
       61 gattctttcc ttcctcaaga gattatcatc aaagtcgagg gagaagacac tgggtctctg
      121 accatcccat ctcaggaagg agtgaacttc aaaattgtga ctgtggactt cacacgggag
      181 gaacagggta cttgcaaccc tgctcagagg accctggaca gagatgtgat cctggagaac
      241 cacagggacc tagtctcttg ggacttggca actgcagttg gaaaaaaaga ttcaacttca
      301 aagcagagga tttttgatga agaaccagct aatggagtga agatagaaag gtttacaagg
      361 gatgatcctt ggttatcttc atgtgaagaa gtggatgatt gtaaagacca gttggagaag
      421 caacaggaaa aacaagagat acttttgcag gaagtggcat tcactcaaag gaaagcagtt
      481 attcatgaga gagtctgcaa aagtgatgaa actggggaga agagtggtct gaattccagt
      541 ctattttcat ccccagttat acccataaga aaccattttc ataaacatgt atcacatgct
      601 aaaaaatggc atcttaatgc tgctgtaaac agtcatcaga agattaatga gaatgagaca
      661 ctatatgaaa ataatgaatg tggaaaaccc cctcagagca ttcaccttat tcagtttaca
      721 agaactcaaa caaaagataa atcctatgga tttagtgacc gtattcaatc tttttgccat
      781 ggtacacccc tacatataca tgaaaaaatt catggaggag gaaaaacctt tgattttaaa
      841 gaatgtgggc aggttttgaa ccccaaaata tcccataatg aacaacagag aattcctttt
      901 gaagagagtc aatataaatg tagtgaaacc tctcatagtt cctcccttac tcaaaacatg
      961 agaaataatt ctgaagagaa accttttgaa tgtaatcagt gtgggaaatc cttcagctgg
     1021 agctcgcatc ttgttgcaca tcagagaact cacacagggg agaaacctta tgaatgtagt
     1081 gaatgtggaa aatccttcag ccggagctcg caccttgttt cccatcagag aactcatact
     1141 ggagagaaac cttacaggtg taatcaatgt gggaaatcct ttagccagag ttatgtcctt
     1201 gttgtgcatc aaagaactca tactggggag aagccttatg aatgcaatca gtgtggaaag
     1261 tcattcaggc agagctataa acttattgca catcaaagaa cacataccgg agagaagccc
     1321 tatgaatgta atcaatgtgg gaaatcattt atccagagct ataaacttat tgcacatcaa
     1381 agaattcata ctggggaaaa accctatgaa tgcaatcagt gtgggaaatc ctttagtcaa
     1441 agttataaac ttgttgctca tcagagaact cacacaggag aaaaaccctt tgaatgtaat
     1501 cagtgtggga aatccttcag ctggagctct cagcttgttg cacatcaaag aactcacact
     1561 ggagagaaac cgtatgaatg tagtgaatgt ggaaaatctt ttaaccgcag ttctcacctt
     1621 gttatgcatc agagaattca cactggggaa aaaccgtatg aatgtaatca gtgtgggaaa
     1681 tccttcagcc agagttatgt tcttgttgta catcagagaa ctcatactgg agaaaagccc
     1741 tatgaatgca gtcaatgtgg gaagtccttc agacagagtt catgccttac tcaacatcag
     1801 agaactcata ctggagagaa accatttgaa tgtaatcagt gtggaaaaac atttagcttg
     1861 agtgctcgac ttattgtgca tcaaagaact catactggag agaaaccctt tacatgtatt
     1921 cagtgtggaa aagctttcat taatagctat aaacttatta ggcatcaggc aactcatact
     1981 gaagagaaac tctatgaatg taactagtaa tgaactagtc cgtaacttga aagtatttcg
     2041 atttcttggc tttatatatc ttgtggaaag gacgatatcg ccggagcgat cagcgaatg
//
