LOCUS       sequence-284463-         983 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1819
FEATURES             Location/Qualifiers
     source          1..983
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggaccact gacatttatg gatgtggcca tagagttctc tttggaggag
       61 tggcagtgcc tggacactgc acagcggaat gtatataggc atgtgatgtt agagaactac
      121 agaaacctgg ttttcttggc tctgtgttct cgttttgccc aagacctttg gctagagcag
      181 aacataaaag attctttcca aaaagtgaca ctgagcagat atggaaaata tggacataag
      241 aatttacagt taagaaaagg ctgtaaaagt gtggatgagt gtaagggaca ccaaggaggt
      301 tttaatggac ttaaccaatg tttgaaaatt accacaagca aaatatttca atgtaataaa
      361 tatgtaaaag tcatgcataa attttcaaat tcaaatagac acaagataag acatactgaa
      421 aataaacatt tcagatgtaa agaatgtgac aaatcacttt gcatgctttc acgcctaact
      481 caacataaaa aaattcatac tagagagaat ttctacaaat gtgaagagtg tggaaaaacc
      541 tttaactggt ccacaaacct ttctaaacct aagaaaattc atactggaga aaaaccctac
      601 aaatgtgaag tatgtggaaa agcctttcac caatcctcaa tccttactaa acataagata
      661 attcgtactg gagaaaaacc ctataaatgt gcacactgtg gcaaagcctt taaacagtcc
      721 tcacacctta ctagacataa gataattcat actgaagaga aaccctacaa atgtgaacaa
      781 tgtggcaagg tctttaagca gtccccaacc cttactaaac atcagataat ttatactgga
      841 gaggaaccat acaaatgtga ggaatgtggc aaagctttta acctatctta gtaatgaact
      901 agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgag
      961 ttgttgtgga gacttaacgc gtt
//
