LOCUS       sequence-284469-        5756 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5756)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 5756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1886
FEATURES             Location/Qualifiers
     source          1..5756
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggatttatc tgctcttcgc gttgaagaag tacaaaatgt cattaatgct
       61 atgcagaaaa tcttagagtg tcccatctgt ctggagttga tcaaggaacc tgtctccaca
      121 aagtgtgacc acatattttg caaattttgc atgctgaaac ttctcaacca gaagaaaggg
      181 ccttcacagt gtcctttatg taagaatgat ataaccaaaa ggagcctaca agaaagtacg
      241 agatttagtc aacttgttga agagctattg aaaatcattt gtgcttttca gcttgacaca
      301 ggtttggagt atgcaaacag ctataatttt gcaaaaaagg aaaataactc tcctgaacat
      361 ctaaaagatg aagtttctat catccaaagt atgggctaca gaaaccgtgc caaaagactt
      421 ctacagagtg aacccgaaaa tccttccttg caggaaacca gtctcagtgt ccaactctct
      481 aaccttggaa ctgtgagaac tctgaggaca aagcagcgga tacaacctca aaagacgtct
      541 gtctacattg aattgggatc tgattcttct gaagataccg ttaataaggc aacttattgc
      601 agtgtgggag atcaagaatt gttacaaatc acccctcaag gaaccaggga tgaaatcagt
      661 ttggattctg caaaaaaggc tgcttgtgaa ttttctgaga cggatgtaac aaatactgaa
      721 catcatcaac ccagtaataa tgatttgaac accactgaga agcgtgcagc tgagaggcat
      781 ccagaaaagt atcagggtag ttctgtttca aacttgcatg tggagccatg tggcacaaat
      841 actcatgcca gctcattaca gcatgagaac agcagtttat tactcactaa agacagaatg
      901 aatgtagaaa aggctgaatt ctgtaataaa agcaaacagc ctggcttagc aaggagccaa
      961 cataacagat gggctggaag taaggaaaca tgtaatgata ggcggactcc cagcacagaa
     1021 aaaaaggtag atctgaatgc tgatcccctg tgtgagagaa aagaatggaa taagcagaaa
     1081 ctgccatgct cagagaatcc tagagatact gaagatgttc cttggataac actaaatagc
     1141 agcattcaga aagttaatga gtggttttcc agaagtgatg aactgttagg ttctgatgac
     1201 tcacatgatg gggagtctga atcaaatgcc aaagtagctg atgtattgga cgttctaaat
     1261 gaggtagatg aatattctgg ttcttcagag aaaatagact tactggccag tgatcctcat
     1321 gaggctttaa tatgtaaaag tgaaagagtt cactccaaat cagtagagag taatattgaa
     1381 gacaaaatat ttgggaaaac ctatcggaag aaggcaagcc tccccaactt aagccatgta
     1441 actgaaaatc taattatagg agcatttgtt actgagccac agataataca agagcgtccc
     1501 ctcacaaata aattaaagcg taaaaggaga cctacatcag gccttcatcc tgaggatttt
     1561 atcaagaaag cagatttggc agttcaaaag actcctgaaa tgataaatca gggaactaac
     1621 caaacggagc agaatggtca agtgatgaat attactaata gtggtcatga gaataaaaca
     1681 aaaggtgatt ctattcagaa tgagaaaaat cctaacccaa tagaatcact cgaaaaagaa
     1741 tctgctttca aaacgaaagc tgaacctata agcagcagta taagcaatat ggaactcgaa
     1801 ttaaatatcc acaattcaaa agcacctaaa aagaataggc tgaggaggaa gtcttctacc
     1861 aggcatattc atgcgcttga actagtagtc agtagaaatc taagcccacc taattgtact
     1921 gaattgcaaa ttgatagttg ttctagcagt gaagagataa agaaaaaaaa gtacaaccaa
     1981 atgccagtca ggcacagcag aaacctacaa ctcatggaag gtaaagaacc tgcaactgga
     2041 gccaagaaga gtaacaagcc aaatgaacag acaagtaaaa gacatgacag cgatactttc
     2101 ccagagctga agttaacaaa tgcacctggt tcttttacta agtgttcaaa taccagtgaa
     2161 cttaaagaat ttgtcaatcc tagccttcca agagaagaaa aagaagagaa actagaaaca
     2221 gttaaagtgt ctaataatgc tgaagacccc aaagatctca tgttaagtgg agaaagggtt
     2281 ttgcaaactg aaagatctgt agagagtagc agtatttcat tggtacctgg tactgattat
     2341 ggcactcagg aaagtatctc gttactggaa gttagcactc tagggaaggc aaaaacagaa
     2401 ccaaataaat gtgtgagtca gtgtgcagca tttgaaaacc ccaagggact aattcatggt
     2461 tgttccaaag ataatagaaa tgacacagaa ggctttaagt atccattggg acatgaagtt
     2521 aaccacagtc gggaaacaag catagaaatg gaagaaagtg aacttgatgc tcagtatttg
     2581 cagaatacat tcaaggtttc aaagcgccag tcatttgctc cgttttcaaa tccaggaaat
     2641 gcagaagagg aatgtgcaac attctctgcc cactctgggt ccttaaagaa acaaagtcca
     2701 aaagtcactt ttgaatgtga acaaaaggaa gaaaatcaag gaaagaatga gtctaatatc
     2761 aagcctgtac agacagttaa tatcactgca ggctttcctg tggttggtca gaaagataag
     2821 ccagttgata atgccaaatg tagtatcaaa ggaggctcta ggttttgtct atcatctcag
     2881 ttcagaggca acgaaactgg actcattact ccaaataaac atggactttt acaaaaccca
     2941 tatcgtatac caccactttt tcccatcaag tcatttgtta aaactaaatg taagaaaaat
     3001 ctgctagagg aaaactttga ggaacattca atgtcacctg aaagagaaat gggaaatgag
     3061 aacattccaa gtacagtgag cacaattagc cgtaataaca ttagagaaaa tgtttttaaa
     3121 gaagccagct caagcaatat taatgaagta ggttccagta ctaatgaagt gggctccagt
     3181 attaatgaaa taggttccag tgatgaaaac attcaagcag aactaggtag aaacagaggg
     3241 ccaaaattga atgctatgct tagattaggg gttttgcaac ctgaggtcta taaacaaagt
     3301 cttcctggaa gtaattgtaa gcatcctgaa ataaaaaagc aagaatatga agaagtagtt
     3361 cagactgtta atacagattt ctctccatat ctgatttcag ataacttaga acagcctatg
     3421 ggaagtagtc atgcatctca ggtttgttct gagacacctg atgacctgtt agatgatggt
     3481 gaaataaagg aagatactag ttttgctgaa aatgacatta aggaaagttc tgctgttttt
     3541 agcaaaagcg tccagaaagg agagcttagc aggagtccta gccctttcac ccatacacat
     3601 ttggctcagg gttaccgaag aggggccaag aaattagagt cctcagaaga gaacttatct
     3661 agtgaggatg aagagcttcc ctgcttccaa cacttgttat ttggtaaagt aaacaatata
     3721 ccttctcagt ctactaggca tagcaccgtt gctaccgagt gtctgtctaa gaacacagag
     3781 gagaatttat tatcattgaa gaatagctta aatgactgca gtaaccaggt aatattggca
     3841 aaggcatctc aggaacatca ccttagtgag gaaacaaaat gttctgctag cttgttttct
     3901 tcacagtgca gtgaattgga agacttgact gcaaatacaa acacccagga tcctttcttg
     3961 attggttctt ccaaacaaat gaggcatcag tctgaaagcc agggagttgg tctgagtgac
     4021 aaggaattgg tttcagatga tgaagaaaga ggaacgggct tggaagaaaa taatcaagaa
     4081 gagcaaagca tggattcaaa cttaggtgaa gcagcatctg ggtgtgagag tgaaacaagc
     4141 gtctctgaag actgctcagg gctatcctct cagagtgaca ttttaaccac tcagcagagg
     4201 gataccatgc aacataacct gataaagctc cagcaggaaa tggctgaact agaagctgtg
     4261 ttagaacagc atgggagcca gccttctaac agctaccctt ccatcataag tgactcttct
     4321 gcccttgagg acctgcgaaa tccagaacaa agcacatcag aaaaagattc gcatatacat
     4381 ggccaaagga acaactccat gttttctaaa aggcctagag aacatatatc agtattaact
     4441 tcacagaaaa gtagtgaata ccctataagc cagaatccag aaggcctttc tgctgacaag
     4501 tttgaggtgt ctgcagatag ttctaccagt aaaaataaag aaccaggagt ggaaaggtca
     4561 tccccttcta aatgcccatc attagatgat aggtggtaca tgcacagttg ctctgggagt
     4621 cttcagaata gaaactaccc atctcaagag gagctcatta aggttgttga tgtggaggag
     4681 caacagctgg aagagtctgg gccacacgat ttgacggaaa catcttactt gccaaggcaa
     4741 gatctagagg gaacccctta cctggaatct ggaatcagcc tcttctctga tgaccctgaa
     4801 tctgatcctt ctgaagacag agccccagag tcagctcgtg ttggcaacat accatcttca
     4861 acctctgcat tgaaagttcc ccaattgaaa gttgcagaat ctgcccagag tccagctgct
     4921 gctcatacta ctgatactgc tgggtataat gcaatggaag aaagtgtgag cagggagaag
     4981 ccagaattga cagcttcaac agaaagggtc aacaaaagaa tgtccatggt ggtgtctggc
     5041 ctgaccccag aagaatttat gctcgtgtac aagtttgcca gaaaacacca catcacttta
     5101 actaatctaa ttactgaaga gactactcat gttgttatga aaacagatgc tgagtttgtg
     5161 tgtgaacgga cactgaaata ttttctagga attgcgggag gaaaatgggt agttagctat
     5221 ttctgggtga cccagtctat taaagaaaga aaaatgctga atgagcatga ttttgaagtc
     5281 agaggagatg tggtcaatgg aagaaaccac caaggtccaa agcgagcaag agaatcccag
     5341 gacagaaaga tcttcagggg gctagaaatc tgttgctatg ggcccttcac caacatgccc
     5401 acagatcaac tggaatggat ggtacagctg tgtggtgctt ctgtggtgaa ggagctttca
     5461 tcattcaccc ttggcacagg tgtccaccca attgtggttg tgcagccaga tgcctggaca
     5521 gaggacaatg gcttccatgc aattgggcag atgtgtgagg cacctgtggt gacccgagag
     5581 tgggtgttgg acagtgtagc actctaccag tgccaggagc tggacaccta cctgataccc
     5641 cagatccccc acagccacta ctagtaatga actagtccgt aacttgaaag tatttcgatt
     5701 tcttggcttt atatatcttg tggaaaggac gatgcttagc tcaggctcaa ctccta
//
