LOCUS       sequence-284472-        2165 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1898
FEATURES             Location/Qualifiers
     source          1..2165
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      716..725
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggagcgggt gaacgacgct tcgtgcggcc cgtctggctg ctacacatac
       61 caggtgagca gacacagcac ggagatgctg cacaacctga accagcagcg caaaaacggc
      121 gggcgcttct gcgacgtgct cttgcgggta ggcgacgaga gcttcccagc gcaccgcgcc
      181 gtgctggccg cctgcagcga gtactttgag tcggtgttca gcgcccagtt gggcgacggc
      241 ggagctgcgg acgggggtcc ggctgatgta gggggcgcga cggcagcacc aggcggcggg
      301 gccgggggca gccgggagct ggagatgcac actatcagct ccaaggtatt tggggacatt
      361 ctggacttcg cctacacttc ccgcatcgtg gtgcgcttgg agagctttcc cgaactcatg
      421 acggccgcca agttcctgct gatgaggtcg gttatcgaga tctgccagga agtcatcaaa
      481 cagtccaacg tacagatcct ggtaccccct gcccgcgccg atataatgct ctttcgcccc
      541 cctgggacct cggacttggg cttccctttg gacatgacca acggggcagc cttggcagcc
      601 aacagcaatg gcatcgccgg cagcatgcag ccagaggagg aggcagctcg ggcggctggt
      661 gcagccattg caggccaagc ctctttgcct gtgttacctg gggtggaccg cttgcccatg
      721 gtggctggac ccctatcccc ccaactgctg acttccccat tccccagtgt ggcatccagt
      781 gcccctcccc tgactggcaa gcgaggccgg ggccgcccaa ggaaggccaa cctgctggac
      841 tcaatgtttg ggtccccagg gggcctgagg gaggcaggca tccttccatg cggtctatgt
      901 ggtaaggtgt tcactgatgc caaccggctc cggcagcacg aggcccagca cggtgtcacc
      961 agcctccagc tgggctacat cgaccttcct cctccgaggc tgggtgagaa tgggctaccc
     1021 atctctgaag accccgacgg cccccgaaag aggagccgga ccaggaagca ggtggcttgt
     1081 gagatctgcg gcaagatctt ccgtgatgtg tatcatctta accggcacaa gctgtcccac
     1141 tctggggaga agccctactc ctgccctgtg tgtgggttgc ggttcaagag aaaagaccgc
     1201 atgtcctacc atgtgcggtc ccatgatggg tccgtgggca agccttacat ctgccagagc
     1261 tgtgggaaag gcttctccag gcctgatcac ttgaacggac atatcaagca ggtgcacact
     1321 tctgagcggc ctcacaagtg tcagacctgc aatgcttctt ttgccacccg agaccgtctg
     1381 cgctcccacc tggcctgtca tgaagacaag gtgccctgcc aggtgtgtgg gaagtacttg
     1441 cgggcagcat acatggcaga ccacctgaag aagcacagcg aggggcccag caacttctgc
     1501 agtatctgta accgaggttt ctcctctgcc tcctacttaa aggtccatgt taaaacccac
     1561 cacggtgttc cccttcccca ggtctccagg caccaggagc ccatcctgaa tgggggagca
     1621 gcgttccact gcgccaggac ctatggcaac aaagaaggcc agaaatgctc acatcaggat
     1681 ccgattgaga gctctgactc ctatggtgac ctctcagatg ccagcgacct gaagacgcca
     1741 gagaagcaga gtgccaatgg ctctttctcc tgcgacatgg cagtccccaa aaacaaaatg
     1801 gagtctgatg gggagaagaa gtacccatgc cctgaatgtg ggagcttctt ccgctctaag
     1861 tcctacttga acaaacacat ccagaaggtg catgtccggg ctctcggggg ccccctgggg
     1921 gacctgggcc ctgcccttgg ctcacctttc tctcctcagc agaacatgtc tctcctcgag
     1981 tcctttgggt ttcagattgt tcagtcggca tttgcgtcat ctttagtaga tcctgaggtt
     2041 gaccagcagc ccatggggcc tgaagggaaa tagtaatgaa ctagtccgta acttgaaagt
     2101 atttcgattt cttggcttta tatatcttgt ggaaaggacg acggttatgt tctccaagat
     2161 ctgct
//
