LOCUS       sequence-284487-        2126 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1987
FEATURES             Location/Qualifiers
     source          1..2126
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcccttac tcaggggcaa ttgtcattca gcgatgtggc catagaattc
       61 tctcaggagg agtggaaatg cctggaccct gggcagaaag ctttatacag ggacgtgatg
      121 ttagagaact acaggaacct ggtctcccta ggtgaggata atgtccgtcc agaagcctgc
      181 atctgctctg gaatctgtct tcctgacctg agtgttacct ccatgttgga gcaaaagaga
      241 gatccctgga ctctgcagag tgaagtgaaa ataataaaca atccagatgg cagggagtgc
      301 atcaaaggtg tgaacacaga gaagagttct aaattgggaa gcagtgcagg aaacaagtca
      361 cttaaaaatc aacatggatt aactcttcag ttacatctga ctgaatggca gccatttcaa
      421 gctgtaagga atatttatgg atgtaagcat gttgagaaat ctatcagtga caattcttca
      481 gtttcaccag ttcaaataag ttttttcagt gtcaaaaccc atatttttaa taactacaga
      541 aatgattttc ttttttctac attactccca caagaacaga aagtacacat tagggaaaag
      601 ccttatggat gtaatgagca tgggaaagtc ttcagagtgt cttcaagcct tactaaccgt
      661 caagtaatcc acattgcaga taaaacttac aaatgtagtg actgtggcga gatctttagt
      721 agcaattcaa actttgcaca acatcaacga atccatactg gagagaagcc ttacaaatat
      781 aatgaatgtg gcaaagtctt caatcagaat tcacaccttg cacaacatca gaaaattcat
      841 actggacaga aaccttacaa taacaaagaa tgtgggaaag tctttagtca ccatgcctac
      901 cttgcacagc ataggaaaat tcatactgga gagaagcctt acaaatgtag tgaatgtggc
      961 aaagcattta gtgtgtgttc cagtcttact gctcatcttg taatccatac tggagagaaa
     1021 ccttatgatt gtaaggaatg tggcaaggtc ttcaggcata agtcttccct aaccactcat
     1081 cagacagttc atactggaga gagaccatac aaatgtaatg aatgtggcaa ggggtttagt
     1141 cgaattgcat tccttgcaag gcatcggaaa gttcatactg gagagaaacc ttacaaatgt
     1201 aatgagtgtg gcaaggtctt tattggcaat tcacgccttg cacgacatag gaaaattcat
     1261 actgggggga ggcgttacaa atgtaatgaa tgtggcaaag catttagaac gtgttcagat
     1321 ctcactgccc atcttctaat ccatactgga gagaaacctt acgaatgtat agactgtggc
     1381 aaggtcttca ggcacaagtc ttccctaacc tatcactgta gaattcatac tggagagaag
     1441 ccttacaaat gtaacgaatg tggcaaggtc ttcagtcaga attcaaacct tcaacgacat
     1501 aggaaaattc atactggaga gaagctttac aaatgtaatg aatgtggcaa ggtcttccgt
     1561 cagaattcac accttgcaca acatagggat attcatactg gagagaagcc ttacagttgt
     1621 aatgaatgtg gcaaggtctt ccgtcggaat tcacaccttg tgcgacatag gaatgttcat
     1681 actggagaaa agccttacag ttgtaatgaa tgtggcaagg tcttcagtcg gaattcacac
     1741 cttgcgcgac ataggaatat tcatactgga gagaagcctc acagttgtaa tgaatgtggc
     1801 aaggtcttca gtcggaattc acaccttgcg cgacatagga aaattcatac tggagagaag
     1861 ctttacaaat gtaatgaatg tagcaaggtc ttcagtcgga attcacgcct tgcacaacat
     1921 aggaatattc atactggagt gaagccttac agttgtaatg aatgtggcaa ggtctttagt
     1981 aaaaattcca tcctagtaca acattgcagt attcatacca gagagaagcc ttagtaatga
     2041 actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
     2101 gactactgcg tatattgtgc gctgcg
//
