LOCUS       sequence-284514-        2252 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2555
FEATURES             Location/Qualifiers
     source          1..2252
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcgcctca gaagcacggc ggtgggggag ggggcggctc ggggcccagc
       61 gcggggtccg ggggaggcgg cttcgggggt tcggcggcgg tggcggcggc gacggcttcg
      121 ggcggcaaat ccggcggcgg gagctgtgga gggggtggca gttactcggc ctcctcctcc
      181 tcctccgcgg cggcagcggc gggggctgcg gtgttaccgg tgaagaagcc gaaaatggag
      241 cacgtccagg ctgaccacga gcttttcctc caggcctttg agaagccaac acagatctat
      301 agatttcttc gaactcggaa tctcatagca ccaatatttt tgcacagaac tcttacttac
      361 atgtctcatc gaaactccag aacaaacatc aaaagcttgt cagctcattt gcagcttacg
      421 tttactggtt tcttccacaa aaatgataag ccatcaccaa actcagaaaa tgaacaaaat
      481 tctgttaccc tggaagtcct gcttgtgaaa gtttgccaca aaaaaagaaa ggatgtaagt
      541 tgtccaataa ggcaagttcc cacaggtaaa aagcaggtgc ctttgaatcc tgacctcaat
      601 caaacaaaac ccggaaattt cccgtccctt gcagtttcca gtaatgaatt tgaacctagt
      661 aacagccata tggtgaagtc ttactcgttg ctatttagag tgactcgtcc aggaagaaga
      721 gagtttaatg gaatgattaa tggagaaacc aatgaaaata ttgatgtcaa tgaagagctt
      781 ccagccagaa gaaaacgaaa tcgtgaggat ggggaaaaga catttgttgc acaaatgaca
      841 gtatttgata aaaacaggcg cttacagctt ttagatgggg aatatgaagt agccatgcag
      901 gaaatggaag aatgtccaat aagcaagaaa agagcaacat gggagactat tcttgatggg
      961 aagaggctgc ctccattcga aacattttct cagggaccta cgttgcagtt cactcttcgt
     1021 tggacaggag agaccaatga taaatctacg gctcctattg ccaaacctct tgccactaga
     1081 aattcagaga gtctccatca ggaaaacaag cctggttcag ttaaacctac tcaaactatt
     1141 gctgttaaag aatcattgac tacagatcta caaacaagaa aagaaaagga tactccaaat
     1201 gaaaaccgac aaaaattaag aatattttat cagtttctct ataacaacaa tacaaggcaa
     1261 caaactgaag caagagatga cctgcattgc ccttggtgta ctctgaactg ccgcaaactt
     1321 tatagtttac tcaagcatct taaactctgc catagcagat ttatcttcaa ctatgtttat
     1381 catccaaaag gtgctaggat agatgtttct atcaatgagt gttatgatgg ctcctatgca
     1441 ggaaatcctc aggatattca tcgccaacct ggatttgctt ttagtcgcaa cggaccagtt
     1501 aagagaacac ctatcacaca tattcttgtg tgcaggccaa aacgaacaaa agcaagcatg
     1561 tctgaatttc ttgaatctga agatggggaa gtagaacagc aaagaacata tagtagtggc
     1621 cacaatcgtc tgtatttcca tagtgatacc tgcttacctc tccgtccaca agaaatggaa
     1681 gtagatagtg aagatgaaaa ggatcctgaa tggctaagag aaaaaaccat tacacaaatt
     1741 gaagagtttt ctgatgttaa tgaaggagag aaagaagtga tgaaactctg gaatctccat
     1801 gtcatgaagc atgggtttat tgctgacaat caaatgaatc atgcctgtat gctgtttgta
     1861 gaaaattatg gacagaaaat aattaagaag aatttatgtc gaaacttcat gcttcatcta
     1921 gtcagcatgc atgactttaa tcttattagc ataatgtcaa tagataaagc tgttaccaag
     1981 ctccgtgaaa tgcagcaaaa attagaaaag ggggaatctg cttcccctgc aaacgaagaa
     2041 ataactgaag aacaaaatgg gacagcaaat ggatttagtg aaattaactc aaaagagaaa
     2101 gctttggaaa cagatagtgt ctcaggggtt tcaaaacaga gcaaaaaaca aaaactctag
     2161 taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
     2221 aaggacgagg agcttaactt atggattgcg aa
//
