LOCUS       sequence-284517-        1682 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2565
FEATURES             Location/Qualifiers
     source          1..1682
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaggaaat accagcccag gaagcagcag ggtcaccaag ggtccagttt
       61 cagtctttgg agacccagtc tgagtgtctg tccccagagc ctcagtttgt gcaggacacc
      121 gacatggaac agggactcac tgggggattt ccaatttcca agcctgatgg gatctcccag
      181 ctggaacagg atctacaggt ctttgatctg gaaactaaga ctagagaagt cttaagagat
      241 gactgctcag atggagagac cagagaagag aacaagctgt tgattcctaa gcagaaaatt
      301 tcggaagaag tgcattcata caaagtgaga gtaggaagac tcaaacacga tattacccaa
      361 gttcctgaga ctagagaagt gtataagtct gaggacagat tagaaagact tcaggaaatt
      421 ctaaggaaat ttctgtacct ggagagagag tttaggcaaa taacaatcag caaggaaacc
      481 ttcaccagtg agaagaacaa tgaatgtcat gaacccgaaa aaagcttcag tctggactct
      541 actattgatg cagatcagag agttcttaga atacagaata ccgatgacaa tgataagtat
      601 gacatgagct tcaaccagaa ttcagcctct ggtaaacatg aacacttaaa tctaacagag
      661 gattttcaga gtagtgaatg taaggaaagc ttaatggatc tctcccacct taataaatgg
      721 gagagcatcc ctaacactga gaaatcctat aaatgtgatg tatgtgggaa aattttccat
      781 cagagctcag cccttactag acatcagaga atccatacta gagagaagcc ctacaaatgt
      841 aaagaatgtg aaaagtcttt cagtcagagc tcaagtctta gtcgacataa aagaatacac
      901 actagagaaa aaccttacaa atgtgaagca tctgataaat cctgtgaagc gtctgataaa
      961 tcctgtagtc caagctcagg cataattcag cataagaaaa ttcacaccag agccaaatct
     1021 tacaaatgta gcagttgtga aagagtcttc agtcgtagtg tccaccttac tcaacatcag
     1081 aaaattcaca aagagatgcc ctgtaagtgt actgtatgtg gcagtgactt ctgccatact
     1141 tcatacctac ttgaacatca gagggtccat catgaagaga aagcctatga gtatgatgaa
     1201 tatgggttgg cctatattaa acaacaagga attcatttca gagaaaagcc ctatacgtgt
     1261 agtgaatgtg gaaaagactt cagattgaat tcacatctta ttcagcatca aagaattcac
     1321 acaggagaga aagcacatga atgtaatgaa tgtggaaaag ctttcagtca aacctcatgc
     1381 cttattcagc atcacaaaat gcataggaaa gagaaatcgt atgaatgtaa tgagtatgag
     1441 ggcagtttca gtcatagctc agatcttatc ctgcaacaag aagtcctcac cagacagaaa
     1501 gcctttgatt gtgatgtatg ggaaaagaac tccagtcaga gagcacatct agttcaacat
     1561 cagagcattc ataccaaaga gaactcatag taatgaacta gtccgtaact tgaaagtatt
     1621 tcgatttctt ggctttatat atcttgtgga aaggacgacc tgttccacgt gtcacggcta
     1681 aa
//
