LOCUS       sequence-284529-        1475 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1475)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1475)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2609
FEATURES             Location/Qualifiers
     source          1..1475
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      154..163
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgctgggagc ggtgaagatg gaagggcacg agccgtccga ctggagcagc
       61 tactatgcag agcccgaggg ctactcctcc gtgagcaaca tgaacgccgg cctggggatg
      121 aacggcatga acacgtacat gagcatgtcg gcggccgcca tgggcagcgg ctcgggcaac
      181 atgagcgcgg gctccatgaa catgtcgtcg tacgtgggcg ctggcatgag cccgtccctg
      241 gcggggatgt cccccggcgc gggcgccatg gcgggcatgg gcggctcggc cggggcggcc
      301 ggcgtggcgg gcatggggcc gcacttgagt cccagcctga gcccgctcgg ggggcaggcg
      361 gccggggcca tgggcggcct ggccccctac gccaacatga actccatgag ccccatgtac
      421 gggcaggcgg gcctgagccg cgcccgcgac cccaagacct acaggcgcag ctacacgcac
      481 gcaaagccgc cctactcgta catctcgctc atcaccatgg ccatccagca gagccccaac
      541 aagatgctga cgctgagcga gatctaccag tggatcatgg acctcttccc cttctaccgg
      601 cagaaccagc agcgctggca gaactccatc cgccactcgc tctccttcaa cgactgtttc
      661 ctgaaggtgc cccgctcgcc cgacaagccc ggcaagggct ccttctggac cctgcaccct
      721 gactcgggca acatgttcga gaacggctgc tacctgcgcc gccagaagcg cttcaagtgc
      781 gagaagcagc tggcgctgaa ggaggccgca ggcgccgccg gcagcggcaa gaaggcggcc
      841 gccggagccc aggcctcaca ggctcaactc ggggaggccg ccgggccggc ctccgagact
      901 ccggcgggca ccgagtcgcc tcactcgagc gcctccccgt gccaggagca caagcgaggg
      961 ggcctgggag agctgaaggg gacgccggct gcggcgctga gccccccaga gccggcgccc
     1021 tctcccgggc agcagcagca ggccgcggcc cacctgctgg gcccgcccca ccacccgggc
     1081 ctgccgcctg aggcccacct gaagccggaa caccactacg ccttcaacca cccgttctcc
     1141 atcaacaacc tcatgtcctc ggagcagcag caccaccaca gccaccacca ccaccaaccc
     1201 cacaaaatgg acctcaaggc ctacgaacag gtgatgcact accccggcta cggttccccc
     1261 atgcctggca gcttggccat gggcccggtc acgaacaaaa cgggcctgga cgcctcgccc
     1321 ctggccgcag atacctccta ctaccagggg gtgtactccc ggcccattat gaactcctct
     1381 tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     1441 ggaaaggacg acagtgttgt ccggtggctt ctgtt
//
