LOCUS       sequence-284541-        2501 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2621
FEATURES             Location/Qualifiers
     source          1..2501
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaaatccc gaagctgctc ccggctcgcg ggacactaca gggcggcggc
       61 ggcggcggta tccccgcggg tggcggccga gtccaccgag gccctgactc gccggctggc
      121 caggtcccca cgcgccgcct cctgctgccc cggggccccc aagatggcgg gcccgggcgg
      181 cggcgcgagg aggccagcac ggcatcacgg ggccctggcc caagcctgtt cgcgccgagg
      241 ccccatcaac ctagcggcgg cggcgacgac ttcttcctgg tgctgcttga cccggtgggt
      301 ggcgacgtgg agaccgcggg ctccggtcag gccgcagggc ctgtgttgag ggaggaggcc
      361 aaggcgggcc cggggctcca gggggacgag agcggcgcga accccgcggg ctgctctgcg
      421 cagggccccc actgcctgtc cgcggttccc actccggccc cgatctccgc ccccggcccc
      481 gccgcggcct tcgcgggcac agtcactatc cacaaccagg acctgctgtt gcgctttgag
      541 aacggcgtcc tcaccctggc cacgccccca ccacacgcct gggagccagg ggccgctcct
      601 gcccagcagc ccaggtgtct gatcgccccc caagctgggt tcccgcaagc cgcgcacccg
      661 ggtgactgcc cagagctgcg gtccgacctc ctgctagccg agcccgcaga acccgcgcct
      721 gctccggcgc cccaggagga ggcggagggc ctggccgccg ccctgggccc ccgcggactg
      781 ctgggctctg gtccaggcgt ggtgctgtac ctgtgccccg aggcgctgtg cgggcaaacc
      841 ttcgccaaga agcaccagct gaagatgcac ctgctgacgc acagcagcag ccagggccag
      901 aggcccttca aatgccccct gggtggctgc ggctggacct tcaccacctc ttacaagctc
      961 aagaggcacc tgcagtcgca cgataaactg cggcccttcg gctgccctgc ggagggctgt
     1021 ggcaagagct tcaccaccgt gtacaacctc aaggcgcaca tgaagggcca tgagcaggag
     1081 aactcgttca aatgtgaggt gtgcgaggag agcttcccca cgcaggccaa actcggcgcc
     1141 caccagcgca gccacttcga acccgagagg ccttaccagt gcgcgttttc tggctgcaag
     1201 aagacattta tcacagtgag tgctctgttt tcccataacc gcgcccattt cagggaacag
     1261 gaactgtttt cctgctcttt ccctggctgc agcaagcaat atgacaaggc ttgtaggctg
     1321 aaaattcacc tgcggagtca caccggcgag agacctttcc tttgtgactt tgatggctgt
     1381 ggctggaact tcaccagcat gtccaaactc ttaaggcaca aaaggaagca cgacgatgac
     1441 cggaggttca tgtgccctgt ggaaggctgt gggaaatctt tcacgagggc cgaacatctg
     1501 aaaggccaca gcattaccca cctgggcaca aagcctttcg tgtgtcctgt ggcaggctgc
     1561 tgtgccaggt tctctgctcg cagtagcctc tacattcact ccaagaaaca cctgcaggat
     1621 gtggacactt ggaaaagccg ttgcccgatc tcctcttgta ataaactctt cacatccaag
     1681 cacagcatga agacgcacat ggttaaaagg cataaggtgg gccaggatct cttagctcag
     1741 ctagaagcag caaattctct tacacccagc agtgaactta ccagccagag acagaatgat
     1801 ctcagtgatg cagagatagt gtctctcttc tctgatgtac ctgacagtac ttctgctgca
     1861 ttgctggaca cagcattggt gaactctgga atcttgacta ttgatgtggc ttctgtgagc
     1921 tcgactctgg cagggcacct ccctgctaat aataataatt ccgtagggca ggctgtggac
     1981 cctccgtcct tgatggccac cagcgaccct cctcaaagtc tggatacctc tctctttttt
     2041 ggaacggcgg ccactggttt tcagcagagc tccttaaata tggatgaggt ctcaagtgta
     2101 agtgtggggc cattgggatc tctggactct ttggccatga aaaactccag tccagagcct
     2161 caggctttga cacccagcag taagctaaca gtggacacag atactctgac tccttcgagc
     2221 accctttgtg aaaacagtgt ctcagaacta ctgacaccag ccaaagcgga gtggagcgta
     2281 catcctaact ctgacttctt tggacaggag ggagaaaccc agtttggatt ccccaatgca
     2341 gcaggaaacc atggttctca gaaagaaaga aatcttatca ctgtgactgg cagctcattt
     2401 ttggtatagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     2461 tcttgtggaa aggacgacgc gcagttcggt aacgtacagc a
//
