LOCUS       sequence-284550-        3617 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3617)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3617)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2667
FEATURES             Location/Qualifiers
     source          1..3617
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggagaataa cttgaaaact tgtcccaaag aggacggtga tttcgtttct
       61 gataaaataa agtttaaaat agaagaggaa gatgatgatg gaattcctcc tgatagtttg
      121 gaaagaatgg actttaaaag cgagcaggag gacatgaagc agacagacag cggtggggag
      181 cgagcgggcc tcggtgggac gggttgcagc tgcaagcccc cggggaagta cttgtctgca
      241 gagagtgagg atgactatgg cgcgctgttc tcccagtaca gcagcaccct atacgacgtg
      301 gccatggagg ccgtgaccca gagcctcctt tccagccgga acatgagctc caggaagaag
      361 tctccagcct ggaagcattt ttttatctct ccccgagaca gcactaaagc aatatgcatg
      421 tactgtgtga aggagttcag cagaggcaaa aacgagaaag acttgagtac cagttgtctc
      481 atgaggcacg tgaggcgcgc acacccgacc gtgctcattc aggaaaatgg cagtgtgtct
      541 gccgtgtcct cgttcccctc tccctcactc ctgcttccac cacagcctgc ggacgcgggt
      601 gacctcagca ccatcctctc acccatcaaa cttgtccaga aagtggcgtc taagatcccg
      661 tcccccgatc gaataacaga ggagtctgtg tctgtagttt cttctgaaga aatctcctct
      721 gacatgtccg tttcggagaa gtgcggcaga gaagaagccc tggtggggtc gtctccccac
      781 ctccctgctc tccattacga tgaacctgca gagaacttag cggagaagag ccttccactt
      841 ccaaagagca cctctgggtc caggagaagg tccgctgtct ggaagcactt ctacctgtcg
      901 ccactggaca actccaaagc tgtctgcatt cactgcatga acgagttcag ccgggggaag
      961 aatgggaagg acctgggcac gagctgcctc atcaggcaca tgtggagggc acaccgcgcc
     1021 atcgtgttgc aggagaacgg gggcacgggc atcccgccac tgtactccac ccctcccact
     1081 ctgctgcctt ccttgctgcc gccggagggg gagctcagct ctgtgtcctc gtctccagta
     1141 aagccggtca gagagtcccc ttcggcctcc tcctcccctg acaggctgac tgaggacttg
     1201 cagtctcact tgaaccctgg agatgggctg atggaagacg tggcggcctt ctcatcttcc
     1261 gatgacatag gggaggcctc ggcgtcctct cctgagaagc agcaggctga tgggctgagt
     1321 cctagattgt ttgaatctgg cgccatcttc cagcagaata aaaaggtcat gaaaagactg
     1381 aagtcggagg tctggcatca cttctccctg gcccccatgg acagcctcaa ggccgagtgt
     1441 cggtactgcg gctgtgccat cagccggggg aagaagggtg atgtgggcac cagctgcctg
     1501 atgagacatc tctaccggcg ccatccagaa gttgtcggga gccagaaggg cttcctgggt
     1561 gcaagtctgg caaactctcc gtatgccact ttggcctctg cagaaagttc ctcttccaaa
     1621 ttgactgact tgccaacagt ggtcacaaaa aacaatcaag ttatgtttcc tgttaatagc
     1681 aaaaagacct cgaagctgtg gaatcatttt tctatttgct ccgcagactc cacaaaagtc
     1741 gtgtgcttgc actgtggccg gaccatcagc cgggggaaga agccgactaa cttgggtacc
     1801 agctgtcttc tgagacattt acagcggttc catagcaacg tgctgaaaac tgaggtctcg
     1861 gagacggctc ggccctcctc tccggacacc cgggtgccgc ggggcacaga attatcaggc
     1921 gcttcctctt ttgatgacac caatgagaag ttttacgatt ctcacccagt tgccaaaaaa
     1981 atcacaagtc tcatagctga aatgattgca cttgacctcc agccatattc ttttgtagac
     2041 aacgttggct ttaacaggct gcttgaatac ttgaaacctc agtactccct ccccgcccct
     2101 tcctacttct ccaggacagc tatcccaggt atgtatgata atgtgaagca gataattatg
     2161 tcccacctta aggaagctga gagtggtgtg atccacttca cgtctggaat atggatgagt
     2221 aaccagaccc gtgagtacct gaccctcacg gcccactggg tttccttcga gtcgccagcc
     2281 cggccgcgct gtgacgacca ccactgctcg gcgctgttgg acgtgtcgca ggtggactgc
     2341 gactacagtg gcaacagcat tcagaagcag ctggagtgct ggtgggaagc gtgggtgacc
     2401 tccaccggcc ttcaggtggg catcaccgtc accgacaacg ccagcatcgg gaagacgctg
     2461 aacgaggggg agcactcgag cgtgcagtgc ttcagccata cggtgaacct gatcgtcagc
     2521 gaggccatta agagccagcg gatggtgcag aacctgctga gcctcgcccg gaagatctgc
     2581 gagcgggtgc accggtcgcc caaggcgaag gagaaactgg ccgagctgca gagggagtac
     2641 gcgctgcctc agcatcacct catccaggac gtcccgtcca agtggagcac ttccttccac
     2701 atgctcgagc ggctcattga gcagaaaagg gccattaacg agatgtccgt cgagtgtaac
     2761 ttccgagagc tgatcagctg cgaccagtgg gaggtcatgc agtccgtgtg ccgtgcgcta
     2821 aagcccttcg aggctgcgag ccgggagatg agcacgcaga tgtccaccct cagccaggtc
     2881 atccccatgg tacacatcct caacaggaag gtggagatgc tcttcgagga gacgatgggc
     2941 atcgacacca tgctgcgctc tctgaaggag gccatggtga gccgcctgtc tgccaccctc
     3001 cacgacccgc ggtacgtctt cgccacgctg ctggatcctc gctacaaggc ctccctgttt
     3061 acggaggagg aggcggagca gtacaaacag gatttaatca gggaactcga actcatgaat
     3121 tctacctcag aggacgtggc tgcctcccac aggtgtgatg ctggctcccc gtcgaaagac
     3181 tctgccgcag aggagaacct gtggtcactt gtggccaagg tgaagaagaa agacccaaga
     3241 gaaaagctgc ctgaagccat ggtgcttgcg tatctggagg aggaggtgct tgaacacagc
     3301 tgtgacccgc tcacctactg gaacctgaag aaggcgtcct ggccggggct gtccgcgctg
     3361 gccgtcagat ttttgggctg ccccccaagc atcgtccctt cagaaaagct gttcaacaca
     3421 cccactgaga acggtagcct tggccaatcc aggctcatga tggaacattt tgaaaaactt
     3481 atctttttga aagtgaatct tcccttaata tactttcagt attagtaatg aactagtccg
     3541 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgataaatgc
     3601 ttactgctgg aattgga
//
