LOCUS       sequence-284559-        1745 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2681
FEATURES             Location/Qualifiers
     source          1..1745
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcacgttt aacaaaaaga cgacaggcgg atacaaaagc tatccagcat
       61 ctttgggcag ccattgagat tatacggaac cagaagcaga ttgccaacat tgaccgtatt
      121 acaaagtata tgtctcgagt ccacggtatg caccctaaag agaccacccg tcagctgagc
      181 ttagctgtga aagatggtct tattgtcgaa actctaacag tgggctgcaa aggttcaaaa
      241 gctggtattg aacaagaagg atattggttg ccaggagatg agattagcat taagaagaag
      301 aatacaaaca aacaggagat gggcacatac ctcagattca ttgtctcccg catgaaggag
      361 agggctatag atcttaataa aaaggggaag gacaataaac acccgatgta caggaggctg
      421 gtgcactcag ctgtggacgt tcccaccatt caagagaaag tgaatgaagg gaaataccga
      481 agttatgaag agttcaaagc tgatgcccaa ttgcttctcc acaataccgt gattttctat
      541 ggagacagtg agcaagctga cattgcgagg atgctatata aagacacatg tcatgagctg
      601 gatgaactgc agctttgcaa gaattgcttt tacttgtcaa atgctcgtcc tgacaactgg
      661 ttctgttatc cttgtatacc taatcatgag ctggtttggg ctaaaatgaa aggttttggg
      721 ttttggccag ccaaagtcat gcagaaagaa gacaatcaag tcgacgttcg cttctttggc
      781 caccaccacc agagggcctg gattccttct gaaaacattc aagatatcac agtcaacatt
      841 catcggctgc acgtgaagcg cagtatgggt tggaaaaagg cctgtgatga gctggagctg
      901 catcagcgtt tcctacgaga agggagattt tggaaatcta agaatgagga ccgaggtgag
      961 gaagaggcag aatccagtat ctcctccacc agtaatgagc agctaaaggt cactcaagaa
     1021 ccaagagcaa agaaaggacg acgtaatcaa agtgtggagc ccaaaaagga agaaccagag
     1081 cctgaaacag aagcagtaag ttctagccag gaaataccca cgatgcctca gcccatcgaa
     1141 aaagtctccg tgtcaactca gacaaagaag ttaagtgcct cttcaccaag aatgctgcat
     1201 cggagcaccc agaccacaaa cgacggcgtg tgtcagagca tgtgccatga caaatacacc
     1261 aagatcttca atgacttcaa agaccggatg aagtcggacc acaagcggga gacagagcgt
     1321 gttgtccgag aagctctgga gaagctgcgt tctgaaatgg aagaagaaaa gagacaagct
     1381 gtaaataaag ctgtagccaa catgcagggt gagatggaca gaaaatgtaa gcaagtaaag
     1441 gaaaagtgta aggaagaatt tgtagaagaa atcaagaagc tggcaacaca gcacaagcaa
     1501 ctgatttctc agaccaagaa gaagcagtgg tgctacaact gtgaggagga ggccatgtac
     1561 cactgctgct ggaacacatc ctactgctcc atcaagtgcc agcaggagca ctggcacgcg
     1621 gagcacaagc gcacctgccg ccggaaaaga tagtaatgaa ctagtccgta acttgaaagt
     1681 atttcgattt cttggcttta tatatcttgt ggaaaggacg atctcctaag atagtttgtt
     1741 ggccg
//
